Gamma band activity in the developing parafascicular nucleus
The parafascicular nucleus (Pf) receives cholinergic input from the pedunculopontine nucleus, part of the reticular activating system involved in waking and rapid eye movement (REM) sleep, and sends projections to the cortex. We tested the hypothesis that Pf neurons fire maximally at gamma band freq...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223077_v107_n3_p772_Kezunovic http://hdl.handle.net/20.500.12110/paper_00223077_v107_n3_p772_Kezunovic |
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paper:paper_00223077_v107_n3_p772_Kezunovic2023-06-08T14:49:11Z Gamma band activity in the developing parafascicular nucleus ω-agatoxin IVA ω-conotoxin-GVIA Arousal Calcium currents Carbachol Gamma oscillations calcium channel N type calcium channel P type calcium channel Q type carbachol omega agatoxin IVA omega agatoxin VIA voltage dependent anion channel aging animal cell animal experiment article brain cortex brain nerve cell calcium signaling cholinergic system controlled study gamma rhythm nerve potential nonhuman patch clamp priority journal rat thalamus parafascicular nucleus Animals Calcium Channel Blockers Calcium Channels, N-Type Carbachol Cholinergic Agonists Intralaminar Thalamic Nuclei Membrane Potentials omega-Agatoxin IVA Patch-Clamp Techniques Rats Rats, Sprague-Dawley The parafascicular nucleus (Pf) receives cholinergic input from the pedunculopontine nucleus, part of the reticular activating system involved in waking and rapid eye movement (REM) sleep, and sends projections to the cortex. We tested the hypothesis that Pf neurons fire maximally at gamma band frequency (30-90 Hz), that this mechanism involves highthreshold voltage-dependent P/Q-and N-type calcium channels, and that this activity is enhanced by the cholinergic agonist carbachol (CAR). Patch-clamped 9-to 25-day-old rat Pf neurons (n = 299) manifested a firing frequency plateau at gamma band when maximally activated (31.5 ± 1.5 Hz) and showed gamma oscillations when voltage-clamped at holding potentials above -20 mV, and the frequency of the oscillations increased significantly with age (24.6 ± 3.8 vs. 51.6 ± 4.4 Hz, P < 0.001) but plateaued at gamma frequencies. Cells exposed to CAR showed significantly higher frequencies early in development compared with those without CAR (24.6 ± 3.8 vs. 41.7 ± 4.3 Hz, P < 0.001) but plateaued with age. The P/Q-type calcium channel blocker ω-agatoxin-IVA (ω-Aga) blocked gamma oscillations, whereas the N-type blocker ω-conotoxin-GVIA (ω-CgTx) only partially decreased the power spectrum amplitude of gamma oscillations. The blocking effect of ω-Aga on P/Q-type currents and ω-CgTx on N-type currents was consistent over age. We conclude that P/Q-and N-type calcium channels appear to mediate Pf gamma oscillations during development. We hypothesize that the cholinergic input to the Pf could activate these cells to oscillate at gamma frequency, and perhaps relay these rhythms to cortical areas, thus providing a stable high-frequency state for "nonspecific" thalamocortical processing. © 2012 the American Physiological Society. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223077_v107_n3_p772_Kezunovic http://hdl.handle.net/20.500.12110/paper_00223077_v107_n3_p772_Kezunovic |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
ω-agatoxin IVA ω-conotoxin-GVIA Arousal Calcium currents Carbachol Gamma oscillations calcium channel N type calcium channel P type calcium channel Q type carbachol omega agatoxin IVA omega agatoxin VIA voltage dependent anion channel aging animal cell animal experiment article brain cortex brain nerve cell calcium signaling cholinergic system controlled study gamma rhythm nerve potential nonhuman patch clamp priority journal rat thalamus parafascicular nucleus Animals Calcium Channel Blockers Calcium Channels, N-Type Carbachol Cholinergic Agonists Intralaminar Thalamic Nuclei Membrane Potentials omega-Agatoxin IVA Patch-Clamp Techniques Rats Rats, Sprague-Dawley |
| spellingShingle |
ω-agatoxin IVA ω-conotoxin-GVIA Arousal Calcium currents Carbachol Gamma oscillations calcium channel N type calcium channel P type calcium channel Q type carbachol omega agatoxin IVA omega agatoxin VIA voltage dependent anion channel aging animal cell animal experiment article brain cortex brain nerve cell calcium signaling cholinergic system controlled study gamma rhythm nerve potential nonhuman patch clamp priority journal rat thalamus parafascicular nucleus Animals Calcium Channel Blockers Calcium Channels, N-Type Carbachol Cholinergic Agonists Intralaminar Thalamic Nuclei Membrane Potentials omega-Agatoxin IVA Patch-Clamp Techniques Rats Rats, Sprague-Dawley Gamma band activity in the developing parafascicular nucleus |
| topic_facet |
ω-agatoxin IVA ω-conotoxin-GVIA Arousal Calcium currents Carbachol Gamma oscillations calcium channel N type calcium channel P type calcium channel Q type carbachol omega agatoxin IVA omega agatoxin VIA voltage dependent anion channel aging animal cell animal experiment article brain cortex brain nerve cell calcium signaling cholinergic system controlled study gamma rhythm nerve potential nonhuman patch clamp priority journal rat thalamus parafascicular nucleus Animals Calcium Channel Blockers Calcium Channels, N-Type Carbachol Cholinergic Agonists Intralaminar Thalamic Nuclei Membrane Potentials omega-Agatoxin IVA Patch-Clamp Techniques Rats Rats, Sprague-Dawley |
| description |
The parafascicular nucleus (Pf) receives cholinergic input from the pedunculopontine nucleus, part of the reticular activating system involved in waking and rapid eye movement (REM) sleep, and sends projections to the cortex. We tested the hypothesis that Pf neurons fire maximally at gamma band frequency (30-90 Hz), that this mechanism involves highthreshold voltage-dependent P/Q-and N-type calcium channels, and that this activity is enhanced by the cholinergic agonist carbachol (CAR). Patch-clamped 9-to 25-day-old rat Pf neurons (n = 299) manifested a firing frequency plateau at gamma band when maximally activated (31.5 ± 1.5 Hz) and showed gamma oscillations when voltage-clamped at holding potentials above -20 mV, and the frequency of the oscillations increased significantly with age (24.6 ± 3.8 vs. 51.6 ± 4.4 Hz, P < 0.001) but plateaued at gamma frequencies. Cells exposed to CAR showed significantly higher frequencies early in development compared with those without CAR (24.6 ± 3.8 vs. 41.7 ± 4.3 Hz, P < 0.001) but plateaued with age. The P/Q-type calcium channel blocker ω-agatoxin-IVA (ω-Aga) blocked gamma oscillations, whereas the N-type blocker ω-conotoxin-GVIA (ω-CgTx) only partially decreased the power spectrum amplitude of gamma oscillations. The blocking effect of ω-Aga on P/Q-type currents and ω-CgTx on N-type currents was consistent over age. We conclude that P/Q-and N-type calcium channels appear to mediate Pf gamma oscillations during development. We hypothesize that the cholinergic input to the Pf could activate these cells to oscillate at gamma frequency, and perhaps relay these rhythms to cortical areas, thus providing a stable high-frequency state for "nonspecific" thalamocortical processing. © 2012 the American Physiological Society. |
| title |
Gamma band activity in the developing parafascicular nucleus |
| title_short |
Gamma band activity in the developing parafascicular nucleus |
| title_full |
Gamma band activity in the developing parafascicular nucleus |
| title_fullStr |
Gamma band activity in the developing parafascicular nucleus |
| title_full_unstemmed |
Gamma band activity in the developing parafascicular nucleus |
| title_sort |
gamma band activity in the developing parafascicular nucleus |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223077_v107_n3_p772_Kezunovic http://hdl.handle.net/20.500.12110/paper_00223077_v107_n3_p772_Kezunovic |
| _version_ |
1768542068114718720 |