Exploring the intrinsic limits of nitrogenase transfer from bacteria to eukaryotes
Biological nitrogen fixation is widespread among the Eubacteria and Archae domains but completely absent in eukaryotes. The lack of lateral transfer of nitrogen-fixation genes from prokaryotes to eukaryotes has been partially attributed to the physiological requirements necessary for the function of...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222844_v77_n1-2_p3_Soto http://hdl.handle.net/20.500.12110/paper_00222844_v77_n1-2_p3_Soto |
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paper:paper_00222844_v77_n1-2_p3_Soto2023-06-08T14:48:48Z Exploring the intrinsic limits of nitrogenase transfer from bacteria to eukaryotes Bacteria Eukaryotes Evolution Lateral transfer Nitrogen fixation Oxygen Bacteria (microorganisms) Eukaryota Medicago sativa Prokaryota Rhizobium Sinorhizobium meliloti nitrogenase alfalfa article bacterium classification eukaryote gene expression regulation genetics metabolism nitrogen fixation nodulation phylogeny Sinorhizobium meliloti symbiosis Bacteria Eukaryota Gene Expression Regulation, Plant Medicago sativa Nitrogen Fixation Nitrogenase Phylogeny Plant Root Nodulation Sinorhizobium meliloti Symbiosis Biological nitrogen fixation is widespread among the Eubacteria and Archae domains but completely absent in eukaryotes. The lack of lateral transfer of nitrogen-fixation genes from prokaryotes to eukaryotes has been partially attributed to the physiological requirements necessary for the function of the nitrogenase complex. However, symbiotic bacterial nitrogenase activity is protected by the nodule, a plant structure whose organogenesis can be trigged in the absence of bacteria. To explore the intrinsic potentiality of this plant organ, we generated rhizobium-independent nodules in alfalfa by overexpressing the MsDMI3 kinase lacking the autoinhibitory domain. These transgenic nodules showed similar levels of leghemoglobin, free oxygen, ATP, and NADPH to those of efficient Sinorhizobium meliloti B399-infected nodules, suggesting that the rhizobium-independent nodules can provide an optimal microenvironment for nitrogenase activity. Finally, we discuss the intrinsic evolutionary constraints on transfer of nitrogen-fixation genes between bacteria and eukaryotes. © 2013 Springer Science+Business Media New York. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222844_v77_n1-2_p3_Soto http://hdl.handle.net/20.500.12110/paper_00222844_v77_n1-2_p3_Soto |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bacteria Eukaryotes Evolution Lateral transfer Nitrogen fixation Oxygen Bacteria (microorganisms) Eukaryota Medicago sativa Prokaryota Rhizobium Sinorhizobium meliloti nitrogenase alfalfa article bacterium classification eukaryote gene expression regulation genetics metabolism nitrogen fixation nodulation phylogeny Sinorhizobium meliloti symbiosis Bacteria Eukaryota Gene Expression Regulation, Plant Medicago sativa Nitrogen Fixation Nitrogenase Phylogeny Plant Root Nodulation Sinorhizobium meliloti Symbiosis |
| spellingShingle |
Bacteria Eukaryotes Evolution Lateral transfer Nitrogen fixation Oxygen Bacteria (microorganisms) Eukaryota Medicago sativa Prokaryota Rhizobium Sinorhizobium meliloti nitrogenase alfalfa article bacterium classification eukaryote gene expression regulation genetics metabolism nitrogen fixation nodulation phylogeny Sinorhizobium meliloti symbiosis Bacteria Eukaryota Gene Expression Regulation, Plant Medicago sativa Nitrogen Fixation Nitrogenase Phylogeny Plant Root Nodulation Sinorhizobium meliloti Symbiosis Exploring the intrinsic limits of nitrogenase transfer from bacteria to eukaryotes |
| topic_facet |
Bacteria Eukaryotes Evolution Lateral transfer Nitrogen fixation Oxygen Bacteria (microorganisms) Eukaryota Medicago sativa Prokaryota Rhizobium Sinorhizobium meliloti nitrogenase alfalfa article bacterium classification eukaryote gene expression regulation genetics metabolism nitrogen fixation nodulation phylogeny Sinorhizobium meliloti symbiosis Bacteria Eukaryota Gene Expression Regulation, Plant Medicago sativa Nitrogen Fixation Nitrogenase Phylogeny Plant Root Nodulation Sinorhizobium meliloti Symbiosis |
| description |
Biological nitrogen fixation is widespread among the Eubacteria and Archae domains but completely absent in eukaryotes. The lack of lateral transfer of nitrogen-fixation genes from prokaryotes to eukaryotes has been partially attributed to the physiological requirements necessary for the function of the nitrogenase complex. However, symbiotic bacterial nitrogenase activity is protected by the nodule, a plant structure whose organogenesis can be trigged in the absence of bacteria. To explore the intrinsic potentiality of this plant organ, we generated rhizobium-independent nodules in alfalfa by overexpressing the MsDMI3 kinase lacking the autoinhibitory domain. These transgenic nodules showed similar levels of leghemoglobin, free oxygen, ATP, and NADPH to those of efficient Sinorhizobium meliloti B399-infected nodules, suggesting that the rhizobium-independent nodules can provide an optimal microenvironment for nitrogenase activity. Finally, we discuss the intrinsic evolutionary constraints on transfer of nitrogen-fixation genes between bacteria and eukaryotes. © 2013 Springer Science+Business Media New York. |
| title |
Exploring the intrinsic limits of nitrogenase transfer from bacteria to eukaryotes |
| title_short |
Exploring the intrinsic limits of nitrogenase transfer from bacteria to eukaryotes |
| title_full |
Exploring the intrinsic limits of nitrogenase transfer from bacteria to eukaryotes |
| title_fullStr |
Exploring the intrinsic limits of nitrogenase transfer from bacteria to eukaryotes |
| title_full_unstemmed |
Exploring the intrinsic limits of nitrogenase transfer from bacteria to eukaryotes |
| title_sort |
exploring the intrinsic limits of nitrogenase transfer from bacteria to eukaryotes |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222844_v77_n1-2_p3_Soto http://hdl.handle.net/20.500.12110/paper_00222844_v77_n1-2_p3_Soto |
| _version_ |
1768546287786917888 |