Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit

The cell cycle has gained attention as a key determinant for cell fate decisions, but the contribution of DNA replication and mitosis in stem cell differentiation has not been extensively studied. To understand if these processes act as “windows of opportunity” for changes in cell identity, we estab...

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Autores principales:	Waisman, Ariel, Cosentino, María Soledad, Barañao, José Lino S., Guberman, Alejandra Sonia
Publicado:	2017
Materias:	cell division DNA replication mouse embryonic stem cells introduction primitive ectoderm-like cells DNA methyltransferase 3A kruppel like factor 4 octamer transcription factor 6 protein p53 transcription factor NANOG transcription factor Otx2 animal cell Article cell cycle cell cycle G1 phase cell cycle regulation cell cycle S phase cell differentiation controlled study mitosis mouse mouse embryonic stem cell nonhuman pluripotent stem cell priority journal stem cell animal genetic transcription physiology Animals Cell Differentiation Cell Division DNA Replication Mice Mouse Embryonic Stem Cells Pluripotent Stem Cells Transcription, Genetic
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222836_v429_n18_p2802_Waisman http://hdl.handle.net/20.500.12110/paper_00222836_v429_n18_p2802_Waisman
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00222836_v429_n18_p2802_Waisman
record_format	dspace
spelling	paper:paper_00222836_v429_n18_p2802_Waisman2023-06-08T14:48:46Z Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit Waisman, Ariel Cosentino, María Soledad Barañao, José Lino S. Guberman, Alejandra Sonia cell division DNA replication mouse embryonic stem cells introduction primitive ectoderm-like cells DNA methyltransferase 3A kruppel like factor 4 octamer transcription factor 6 protein p53 transcription factor NANOG transcription factor Otx2 animal cell Article cell cycle cell cycle G1 phase cell cycle regulation cell cycle S phase cell differentiation cell division controlled study DNA replication mitosis mouse mouse embryonic stem cell nonhuman pluripotent stem cell priority journal stem cell animal cell differentiation genetic transcription physiology pluripotent stem cell Animals Cell Differentiation Cell Division DNA Replication Mice Mouse Embryonic Stem Cells Pluripotent Stem Cells Transcription, Genetic The cell cycle has gained attention as a key determinant for cell fate decisions, but the contribution of DNA replication and mitosis in stem cell differentiation has not been extensively studied. To understand if these processes act as “windows of opportunity” for changes in cell identity, we established synchronized cultures of mouse embryonic stem cells as they exit the ground state of pluripotency. We show that initial transcriptional changes in this transition do not require passage through mitosis and that conversion to primed pluripotency is linked to lineage priming in the G1 phase. Importantly, we demonstrate that impairment of DNA replication severely blocks transcriptional switch to primed pluripotency, even in the absence of p53 activity induced by the DNA damage response. Our data suggest an important role for DNA replication during mouse embryonic stem cell differentiation, which could shed light on why pluripotent cells are only receptive to differentiation signals during G1, that is, before the S phase. © 2017 Elsevier Ltd Fil:Waisman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cosentino, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guberman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222836_v429_n18_p2802_Waisman http://hdl.handle.net/20.500.12110/paper_00222836_v429_n18_p2802_Waisman
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cell division DNA replication mouse embryonic stem cells introduction primitive ectoderm-like cells DNA methyltransferase 3A kruppel like factor 4 octamer transcription factor 6 protein p53 transcription factor NANOG transcription factor Otx2 animal cell Article cell cycle cell cycle G1 phase cell cycle regulation cell cycle S phase cell differentiation cell division controlled study DNA replication mitosis mouse mouse embryonic stem cell nonhuman pluripotent stem cell priority journal stem cell animal cell differentiation genetic transcription physiology pluripotent stem cell Animals Cell Differentiation Cell Division DNA Replication Mice Mouse Embryonic Stem Cells Pluripotent Stem Cells Transcription, Genetic
spellingShingle	cell division DNA replication mouse embryonic stem cells introduction primitive ectoderm-like cells DNA methyltransferase 3A kruppel like factor 4 octamer transcription factor 6 protein p53 transcription factor NANOG transcription factor Otx2 animal cell Article cell cycle cell cycle G1 phase cell cycle regulation cell cycle S phase cell differentiation cell division controlled study DNA replication mitosis mouse mouse embryonic stem cell nonhuman pluripotent stem cell priority journal stem cell animal cell differentiation genetic transcription physiology pluripotent stem cell Animals Cell Differentiation Cell Division DNA Replication Mice Mouse Embryonic Stem Cells Pluripotent Stem Cells Transcription, Genetic Waisman, Ariel Cosentino, María Soledad Barañao, José Lino S. Guberman, Alejandra Sonia Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit
topic_facet	cell division DNA replication mouse embryonic stem cells introduction primitive ectoderm-like cells DNA methyltransferase 3A kruppel like factor 4 octamer transcription factor 6 protein p53 transcription factor NANOG transcription factor Otx2 animal cell Article cell cycle cell cycle G1 phase cell cycle regulation cell cycle S phase cell differentiation cell division controlled study DNA replication mitosis mouse mouse embryonic stem cell nonhuman pluripotent stem cell priority journal stem cell animal cell differentiation genetic transcription physiology pluripotent stem cell Animals Cell Differentiation Cell Division DNA Replication Mice Mouse Embryonic Stem Cells Pluripotent Stem Cells Transcription, Genetic
description	The cell cycle has gained attention as a key determinant for cell fate decisions, but the contribution of DNA replication and mitosis in stem cell differentiation has not been extensively studied. To understand if these processes act as “windows of opportunity” for changes in cell identity, we established synchronized cultures of mouse embryonic stem cells as they exit the ground state of pluripotency. We show that initial transcriptional changes in this transition do not require passage through mitosis and that conversion to primed pluripotency is linked to lineage priming in the G1 phase. Importantly, we demonstrate that impairment of DNA replication severely blocks transcriptional switch to primed pluripotency, even in the absence of p53 activity induced by the DNA damage response. Our data suggest an important role for DNA replication during mouse embryonic stem cell differentiation, which could shed light on why pluripotent cells are only receptive to differentiation signals during G1, that is, before the S phase. © 2017 Elsevier Ltd
author	Waisman, Ariel Cosentino, María Soledad Barañao, José Lino S. Guberman, Alejandra Sonia
author_facet	Waisman, Ariel Cosentino, María Soledad Barañao, José Lino S. Guberman, Alejandra Sonia
author_sort	Waisman, Ariel
title	Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit
title_short	Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit
title_full	Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit
title_fullStr	Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit
title_full_unstemmed	Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit
title_sort	inhibition of cell division and dna replication impair mouse-naïve pluripotency exit
publishDate	2017
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222836_v429_n18_p2802_Waisman http://hdl.handle.net/20.500.12110/paper_00222836_v429_n18_p2802_Waisman
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Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit

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