Electropore Formation in Mechanically Constrained Phospholipid Bilayers
Molecular dynamics simulations of lipid bilayers in aqueous systems reveal how an applied electric field stabilizes the reorganization of the water–membrane interface into water-filled, membrane-spanning, conductive pores with a symmetric, toroidal geometry. The pore formation process and the result...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222631_v251_n2_p237_Fernandez http://hdl.handle.net/20.500.12110/paper_00222631_v251_n2_p237_Fernandez |
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paper:paper_00222631_v251_n2_p237_Fernandez2023-06-08T14:48:39Z Electropore Formation in Mechanically Constrained Phospholipid Bilayers Electroporation Molecular dynamics Phospholipid bilayer Position constraints 2 oleoyl 1 palmitoylphosphatidylcholine Article cell membrane channel gating covalent bond electric field electroporation extracellular matrix mechanical constraint molecular dynamics molecular model particle size phospholipid bilayer protein assembly simulation Molecular dynamics simulations of lipid bilayers in aqueous systems reveal how an applied electric field stabilizes the reorganization of the water–membrane interface into water-filled, membrane-spanning, conductive pores with a symmetric, toroidal geometry. The pore formation process and the resulting symmetric structures are consistent with other mathematical approaches such as continuum models formulated to describe the electroporation process. Some experimental data suggest, however, that the shape of lipid electropores in living cell membranes may be asymmetric. We describe here the axially asymmetric pores that form when mechanical constraints are applied to selected phospholipid atoms. Electropore formation proceeds even with severe constraints in place, but pore shape and pore formation time are affected. Since lateral and transverse movement of phospholipids may be restricted in cell membranes by covalent attachments to or non-covalent associations with other components of the membrane or to membrane-proximate intracellular or extracellular biomolecular assemblies, these lipid-constrained molecular models point the way to more realistic representations of cell membranes in electric fields. © 2017, Springer Science+Business Media, LLC, part of Springer Nature. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222631_v251_n2_p237_Fernandez http://hdl.handle.net/20.500.12110/paper_00222631_v251_n2_p237_Fernandez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Electroporation Molecular dynamics Phospholipid bilayer Position constraints 2 oleoyl 1 palmitoylphosphatidylcholine Article cell membrane channel gating covalent bond electric field electroporation extracellular matrix mechanical constraint molecular dynamics molecular model particle size phospholipid bilayer protein assembly simulation |
| spellingShingle |
Electroporation Molecular dynamics Phospholipid bilayer Position constraints 2 oleoyl 1 palmitoylphosphatidylcholine Article cell membrane channel gating covalent bond electric field electroporation extracellular matrix mechanical constraint molecular dynamics molecular model particle size phospholipid bilayer protein assembly simulation Electropore Formation in Mechanically Constrained Phospholipid Bilayers |
| topic_facet |
Electroporation Molecular dynamics Phospholipid bilayer Position constraints 2 oleoyl 1 palmitoylphosphatidylcholine Article cell membrane channel gating covalent bond electric field electroporation extracellular matrix mechanical constraint molecular dynamics molecular model particle size phospholipid bilayer protein assembly simulation |
| description |
Molecular dynamics simulations of lipid bilayers in aqueous systems reveal how an applied electric field stabilizes the reorganization of the water–membrane interface into water-filled, membrane-spanning, conductive pores with a symmetric, toroidal geometry. The pore formation process and the resulting symmetric structures are consistent with other mathematical approaches such as continuum models formulated to describe the electroporation process. Some experimental data suggest, however, that the shape of lipid electropores in living cell membranes may be asymmetric. We describe here the axially asymmetric pores that form when mechanical constraints are applied to selected phospholipid atoms. Electropore formation proceeds even with severe constraints in place, but pore shape and pore formation time are affected. Since lateral and transverse movement of phospholipids may be restricted in cell membranes by covalent attachments to or non-covalent associations with other components of the membrane or to membrane-proximate intracellular or extracellular biomolecular assemblies, these lipid-constrained molecular models point the way to more realistic representations of cell membranes in electric fields. © 2017, Springer Science+Business Media, LLC, part of Springer Nature. |
| title |
Electropore Formation in Mechanically Constrained Phospholipid Bilayers |
| title_short |
Electropore Formation in Mechanically Constrained Phospholipid Bilayers |
| title_full |
Electropore Formation in Mechanically Constrained Phospholipid Bilayers |
| title_fullStr |
Electropore Formation in Mechanically Constrained Phospholipid Bilayers |
| title_full_unstemmed |
Electropore Formation in Mechanically Constrained Phospholipid Bilayers |
| title_sort |
electropore formation in mechanically constrained phospholipid bilayers |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222631_v251_n2_p237_Fernandez http://hdl.handle.net/20.500.12110/paper_00222631_v251_n2_p237_Fernandez |
| _version_ |
1768545314471411712 |