Neutrophil signaling pathways activated by bacterial DNA stimulation

We have previously shown that bacterial DNA activates human neutrophils in a CpG-independent manner. In this study, we have characterized the signaling pathways involved in the activation mechanism. We found that p38 MAPK, ERK1/2, and JNK pathways, as well as the PI3K/Akt pathway, are activated by b...

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Autores principales:	Costas, Mónica Alejandra, Coso, Omar Adrian, Gamberale, Romina, Vermeulen, Mónica, Salamone, Gabriela V., Tanos, Tamara, Trevani, Analía S.
Publicado:	2006
Materias:	bacterial DNA CD11b antigen immunoglobulin enhancer binding protein interleukin 1 receptor associated kinase 1 interleukin 8 mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase inhibitor mitogen activated protein kinase p38 mitogen activated protein kinase p38 inhibitor myeloid differentiation factor 88 phosphatidylinositol 3 kinase protein kinase B stress activated protein kinase stress activated protein kinase inhibitor toll like receptor 9 transcription factor AP 1 animal cell animal experiment article controlled study cytokine production cytokine release enzyme activation enzyme activity enzyme degradation enzyme inhibition gene human human cell immunomodulation leukocyte activation male mouse myd88 gene neutrophil nonhuman normal human priority journal signal transduction tlr9 gene
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221767_v177_n6_p4037_Alvarez http://hdl.handle.net/20.500.12110/paper_00221767_v177_n6_p4037_Alvarez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_00221767_v177_n6_p4037_Alvarez2023-06-08T14:46:55Z Neutrophil signaling pathways activated by bacterial DNA stimulation Costas, Mónica Alejandra Coso, Omar Adrian Gamberale, Romina Vermeulen, Mónica Salamone, Gabriela V. Tanos, Tamara Trevani, Analía S. bacterial DNA CD11b antigen immunoglobulin enhancer binding protein interleukin 1 receptor associated kinase 1 interleukin 8 mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase inhibitor mitogen activated protein kinase p38 mitogen activated protein kinase p38 inhibitor myeloid differentiation factor 88 phosphatidylinositol 3 kinase protein kinase B stress activated protein kinase stress activated protein kinase inhibitor toll like receptor 9 transcription factor AP 1 animal cell animal experiment article controlled study cytokine production cytokine release enzyme activation enzyme activity enzyme degradation enzyme inhibition gene human human cell immunomodulation leukocyte activation male mouse myd88 gene neutrophil nonhuman normal human priority journal signal transduction tlr9 gene We have previously shown that bacterial DNA activates human neutrophils in a CpG-independent manner. In this study, we have characterized the signaling pathways involved in the activation mechanism. We found that p38 MAPK, ERK1/2, and JNK pathways, as well as the PI3K/Akt pathway, are activated by bacterial DNA. We also determined that bacterial DNA induces NF-κB and AP-1 activation. When analyzing the role of these pathways on neutrophil functions, we observed that up-regulation of CD11b triggered by bacterial DNA was decreased by pharmacological inhibitors of the p38 MAPK, ERK1/2, and JNK, whereas stimulation of IL-8 release was dependent on p38, ERK1/2, and NF-κB. Moreover, we found that IL-8 production was markedly enhanced by inhibition of JNK, suggesting that this pathway negatively modulates NF-κB-dependent transcription. We also observed that bacterial DNA stimulated IL-1R-associated kinase-1 kinase activity and its partial degradation. Finally, we determined that bacterial DNA stimulated CD11b up-regulation in TLR9 -/- but not in MyD88 -/- mouse neutrophils, supporting that bacterial DNA induces neutrophil activation through a TLR9-independent and MyD88-dependent pathway. Copyright © 2006 by The American Association of Immunologists, Inc. Fil:Costas, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coso, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gamberale, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vermeulen, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Salamone, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tanos, T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Trevani, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221767_v177_n6_p4037_Alvarez http://hdl.handle.net/20.500.12110/paper_00221767_v177_n6_p4037_Alvarez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	bacterial DNA CD11b antigen immunoglobulin enhancer binding protein interleukin 1 receptor associated kinase 1 interleukin 8 mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase inhibitor mitogen activated protein kinase p38 mitogen activated protein kinase p38 inhibitor myeloid differentiation factor 88 phosphatidylinositol 3 kinase protein kinase B stress activated protein kinase stress activated protein kinase inhibitor toll like receptor 9 transcription factor AP 1 animal cell animal experiment article controlled study cytokine production cytokine release enzyme activation enzyme activity enzyme degradation enzyme inhibition gene human human cell immunomodulation leukocyte activation male mouse myd88 gene neutrophil nonhuman normal human priority journal signal transduction tlr9 gene
spellingShingle	bacterial DNA CD11b antigen immunoglobulin enhancer binding protein interleukin 1 receptor associated kinase 1 interleukin 8 mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase inhibitor mitogen activated protein kinase p38 mitogen activated protein kinase p38 inhibitor myeloid differentiation factor 88 phosphatidylinositol 3 kinase protein kinase B stress activated protein kinase stress activated protein kinase inhibitor toll like receptor 9 transcription factor AP 1 animal cell animal experiment article controlled study cytokine production cytokine release enzyme activation enzyme activity enzyme degradation enzyme inhibition gene human human cell immunomodulation leukocyte activation male mouse myd88 gene neutrophil nonhuman normal human priority journal signal transduction tlr9 gene Costas, Mónica Alejandra Coso, Omar Adrian Gamberale, Romina Vermeulen, Mónica Salamone, Gabriela V. Tanos, Tamara Trevani, Analía S. Neutrophil signaling pathways activated by bacterial DNA stimulation
topic_facet	bacterial DNA CD11b antigen immunoglobulin enhancer binding protein interleukin 1 receptor associated kinase 1 interleukin 8 mitogen activated protein kinase 1 mitogen activated protein kinase 3 mitogen activated protein kinase inhibitor mitogen activated protein kinase p38 mitogen activated protein kinase p38 inhibitor myeloid differentiation factor 88 phosphatidylinositol 3 kinase protein kinase B stress activated protein kinase stress activated protein kinase inhibitor toll like receptor 9 transcription factor AP 1 animal cell animal experiment article controlled study cytokine production cytokine release enzyme activation enzyme activity enzyme degradation enzyme inhibition gene human human cell immunomodulation leukocyte activation male mouse myd88 gene neutrophil nonhuman normal human priority journal signal transduction tlr9 gene
description	We have previously shown that bacterial DNA activates human neutrophils in a CpG-independent manner. In this study, we have characterized the signaling pathways involved in the activation mechanism. We found that p38 MAPK, ERK1/2, and JNK pathways, as well as the PI3K/Akt pathway, are activated by bacterial DNA. We also determined that bacterial DNA induces NF-κB and AP-1 activation. When analyzing the role of these pathways on neutrophil functions, we observed that up-regulation of CD11b triggered by bacterial DNA was decreased by pharmacological inhibitors of the p38 MAPK, ERK1/2, and JNK, whereas stimulation of IL-8 release was dependent on p38, ERK1/2, and NF-κB. Moreover, we found that IL-8 production was markedly enhanced by inhibition of JNK, suggesting that this pathway negatively modulates NF-κB-dependent transcription. We also observed that bacterial DNA stimulated IL-1R-associated kinase-1 kinase activity and its partial degradation. Finally, we determined that bacterial DNA stimulated CD11b up-regulation in TLR9 -/- but not in MyD88 -/- mouse neutrophils, supporting that bacterial DNA induces neutrophil activation through a TLR9-independent and MyD88-dependent pathway. Copyright © 2006 by The American Association of Immunologists, Inc.
author	Costas, Mónica Alejandra Coso, Omar Adrian Gamberale, Romina Vermeulen, Mónica Salamone, Gabriela V. Tanos, Tamara Trevani, Analía S.
author_facet	Costas, Mónica Alejandra Coso, Omar Adrian Gamberale, Romina Vermeulen, Mónica Salamone, Gabriela V. Tanos, Tamara Trevani, Analía S.
author_sort	Costas, Mónica Alejandra
title	Neutrophil signaling pathways activated by bacterial DNA stimulation
title_short	Neutrophil signaling pathways activated by bacterial DNA stimulation
title_full	Neutrophil signaling pathways activated by bacterial DNA stimulation
title_fullStr	Neutrophil signaling pathways activated by bacterial DNA stimulation
title_full_unstemmed	Neutrophil signaling pathways activated by bacterial DNA stimulation
title_sort	neutrophil signaling pathways activated by bacterial dna stimulation
publishDate	2006
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221767_v177_n6_p4037_Alvarez http://hdl.handle.net/20.500.12110/paper_00221767_v177_n6_p4037_Alvarez
work_keys_str_mv	AT costasmonicaalejandra neutrophilsignalingpathwaysactivatedbybacterialdnastimulation AT cosoomaradrian neutrophilsignalingpathwaysactivatedbybacterialdnastimulation AT gamberaleromina neutrophilsignalingpathwaysactivatedbybacterialdnastimulation AT vermeulenmonica neutrophilsignalingpathwaysactivatedbybacterialdnastimulation AT salamonegabrielav neutrophilsignalingpathwaysactivatedbybacterialdnastimulation AT tanostamara neutrophilsignalingpathwaysactivatedbybacterialdnastimulation AT trevanianalias neutrophilsignalingpathwaysactivatedbybacterialdnastimulation
_version_	1768545632754073600

Neutrophil signaling pathways activated by bacterial DNA stimulation

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