Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons

The karyotype of the neotropical primate genus Cebus (Platyrrhini: Cebidae), considered the most ancestral one, shows the greatest amount of heterochromatin described among Platyrrhini genera. Banding techniques and restriction enzyme digestion have previously revealed great variability of quantity...

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Autores principales:	Nieves, Mariela, Mudry, Marta Dolores
Publicado:	2011
Materias:	Cebus chromosome microdissection FISH heterochromatin Platyrrhini primate evolution animal article chromosome chromosome banding pattern comparative study fluorescence in situ hybridization genetics karyotyping microdissection nucleotide repeat Animals Chromosome Banding Chromosomes Heterochromatin In Situ Hybridization, Fluorescence Karyotyping Microdissection Repetitive Sequences, Nucleic Acid Callitrichidae Cebidae Cebus albifrons Cebus apella Cebus kaapori Cebus libidinosus Cebus olivaceus Cebus xanthosternos Primates
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221333_v90_n1_p111_Nieves http://hdl.handle.net/20.500.12110/paper_00221333_v90_n1_p111_Nieves
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00221333_v90_n1_p111_Nieves
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spelling	paper:paper_00221333_v90_n1_p111_Nieves2025-07-30T17:28:46Z Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons Nieves, Mariela Mudry, Marta Dolores Cebus chromosome microdissection FISH heterochromatin Platyrrhini primate evolution animal article Cebus chromosome chromosome banding pattern comparative study fluorescence in situ hybridization genetics heterochromatin karyotyping microdissection nucleotide repeat Animals Cebus Chromosome Banding Chromosomes Heterochromatin In Situ Hybridization, Fluorescence Karyotyping Microdissection Repetitive Sequences, Nucleic Acid Callitrichidae Cebidae Cebus Cebus albifrons Cebus apella Cebus kaapori Cebus libidinosus Cebus olivaceus Cebus xanthosternos Platyrrhini Primates The karyotype of the neotropical primate genus Cebus (Platyrrhini: Cebidae), considered the most ancestral one, shows the greatest amount of heterochromatin described among Platyrrhini genera. Banding techniques and restriction enzyme digestion have previously revealed great variability of quantity and composition of heterochromatin in this genus. In this context, we use fluorescence in situ hybridization (FISH) to analyse this genomic region and discuss its possible role in the diversification of Cebus. We used a heterochromatin probe for chromosome 11 of Cebus libidinosus (11qHe+ CLI probe), obtained by chromosome microdissection. Twenty-six specimens belonging to the families Atelidae, Cebidae, Callitrichidae and Pithecidae (Platyrrhini) were studied. Fourteen out of 26 specimens were Cebus (Cebidae) individuals of C. libidinosus, C. xanthosternos, C. apella, C. nigritus, C. albifrons, C. kaapori and C. olivaceus. In Cebus specimens, we found 6 to 22 positive signals located in interstitial and telomeric positions along the different species. No hybridization signal was observed among the remaining Ceboidea species, thus reinforcing the idea of a Cebus-specific heterochromatin composed of a complex system of repetitive sequences. © 2011 Indian Academy of Sciences. Fil:Nieves, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221333_v90_n1_p111_Nieves http://hdl.handle.net/20.500.12110/paper_00221333_v90_n1_p111_Nieves
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cebus chromosome microdissection FISH heterochromatin Platyrrhini primate evolution animal article Cebus chromosome chromosome banding pattern comparative study fluorescence in situ hybridization genetics heterochromatin karyotyping microdissection nucleotide repeat Animals Cebus Chromosome Banding Chromosomes Heterochromatin In Situ Hybridization, Fluorescence Karyotyping Microdissection Repetitive Sequences, Nucleic Acid Callitrichidae Cebidae Cebus Cebus albifrons Cebus apella Cebus kaapori Cebus libidinosus Cebus olivaceus Cebus xanthosternos Platyrrhini Primates
spellingShingle	Cebus chromosome microdissection FISH heterochromatin Platyrrhini primate evolution animal article Cebus chromosome chromosome banding pattern comparative study fluorescence in situ hybridization genetics heterochromatin karyotyping microdissection nucleotide repeat Animals Cebus Chromosome Banding Chromosomes Heterochromatin In Situ Hybridization, Fluorescence Karyotyping Microdissection Repetitive Sequences, Nucleic Acid Callitrichidae Cebidae Cebus Cebus albifrons Cebus apella Cebus kaapori Cebus libidinosus Cebus olivaceus Cebus xanthosternos Platyrrhini Primates Nieves, Mariela Mudry, Marta Dolores Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons
topic_facet	Cebus chromosome microdissection FISH heterochromatin Platyrrhini primate evolution animal article Cebus chromosome chromosome banding pattern comparative study fluorescence in situ hybridization genetics heterochromatin karyotyping microdissection nucleotide repeat Animals Cebus Chromosome Banding Chromosomes Heterochromatin In Situ Hybridization, Fluorescence Karyotyping Microdissection Repetitive Sequences, Nucleic Acid Callitrichidae Cebidae Cebus Cebus albifrons Cebus apella Cebus kaapori Cebus libidinosus Cebus olivaceus Cebus xanthosternos Platyrrhini Primates
description	The karyotype of the neotropical primate genus Cebus (Platyrrhini: Cebidae), considered the most ancestral one, shows the greatest amount of heterochromatin described among Platyrrhini genera. Banding techniques and restriction enzyme digestion have previously revealed great variability of quantity and composition of heterochromatin in this genus. In this context, we use fluorescence in situ hybridization (FISH) to analyse this genomic region and discuss its possible role in the diversification of Cebus. We used a heterochromatin probe for chromosome 11 of Cebus libidinosus (11qHe+ CLI probe), obtained by chromosome microdissection. Twenty-six specimens belonging to the families Atelidae, Cebidae, Callitrichidae and Pithecidae (Platyrrhini) were studied. Fourteen out of 26 specimens were Cebus (Cebidae) individuals of C. libidinosus, C. xanthosternos, C. apella, C. nigritus, C. albifrons, C. kaapori and C. olivaceus. In Cebus specimens, we found 6 to 22 positive signals located in interstitial and telomeric positions along the different species. No hybridization signal was observed among the remaining Ceboidea species, thus reinforcing the idea of a Cebus-specific heterochromatin composed of a complex system of repetitive sequences. © 2011 Indian Academy of Sciences.
author	Nieves, Mariela Mudry, Marta Dolores
author_facet	Nieves, Mariela Mudry, Marta Dolores
author_sort	Nieves, Mariela
title	Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons
title_short	Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons
title_full	Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons
title_fullStr	Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons
title_full_unstemmed	Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons
title_sort	analysis of the heterochromatin of cebus (primates, platyrrhini) by micro-fish and banding pattern comparisons
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221333_v90_n1_p111_Nieves http://hdl.handle.net/20.500.12110/paper_00221333_v90_n1_p111_Nieves
work_keys_str_mv	AT nievesmariela analysisoftheheterochromatinofcebusprimatesplatyrrhinibymicrofishandbandingpatterncomparisons AT mudrymartadolores analysisoftheheterochromatinofcebusprimatesplatyrrhinibymicrofishandbandingpatterncomparisons
_version_	1840321414073155584

Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons

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