Involvement of the cytoskeleton in Junin virus multiplication

The role of the cellular cytoskeleton in Junin virus (JV) infection was explored in two ways. Firstly, the action of inhibitors that affect individual cytoskeletal systems (microtubules or microfilaments) selectively was analysed. It was found that perturbations of microtubule or microfilament netwo...

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Autores principales:	Candurra, Nélida Alicia, Lago, María José, Maskin, Laura, Damonte, Elsa Beatriz
Publicado:	1999
Materias:	colchicine dimethyl sulfoxide egtazic acid nifedipine nocodazole octyl glucoside triton x 100 virus envelope protein virus glycoprotein virus protein animal cell article cytoskeleton Junin virus microfilament microtubule nonhuman priority journal Vero cell virogenesis Animalia RNA viruses
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v80_n1_p147_Candurra http://hdl.handle.net/20.500.12110/paper_00221317_v80_n1_p147_Candurra
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00221317_v80_n1_p147_Candurra
record_format	dspace
spelling	paper:paper_00221317_v80_n1_p147_Candurra2023-06-08T14:46:31Z Involvement of the cytoskeleton in Junin virus multiplication Candurra, Nélida Alicia Lago, María José Maskin, Laura Damonte, Elsa Beatriz colchicine dimethyl sulfoxide egtazic acid nifedipine nocodazole octyl glucoside triton x 100 virus envelope protein virus glycoprotein virus protein animal cell article cytoskeleton Junin virus microfilament microtubule nonhuman priority journal Vero cell virogenesis Animalia Junin virus RNA viruses The role of the cellular cytoskeleton in Junin virus (JV) infection was explored in two ways. Firstly, the action of inhibitors that affect individual cytoskeletal systems (microtubules or microfilaments) selectively was analysed. It was found that perturbations of microtubule or microfilament networks caused by colchicine, nocodazole, nifedipine, EGTA or DMSO strongly affected virion production and viral protein expression at non-cytotoxic concentrations. Secondly, the extent of association of viral proteins and infectious virus particles with the cytoskeletal fraction of monkey Vero cells was determined by using three non-ionic detergents, Triton X-100 (TX-100), NP-40 and octyl glucoside (OG). The cytoskeleton retained nearly 70% of the external JV envelope glycoprotein GP38 and about 40% of the JV nucleoprotein NP, according to TX-100 and OG insolubility results. Furthermore, 1% of the total cell-bound infectivity was detected in the detergent-insoluble fraction, suggesting that cytoskeletal components are involved in the initiation of the assembly and budding processes of JV particles at the plasma membrane. Fil:Candurra, N.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lago, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maskin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v80_n1_p147_Candurra http://hdl.handle.net/20.500.12110/paper_00221317_v80_n1_p147_Candurra
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	colchicine dimethyl sulfoxide egtazic acid nifedipine nocodazole octyl glucoside triton x 100 virus envelope protein virus glycoprotein virus protein animal cell article cytoskeleton Junin virus microfilament microtubule nonhuman priority journal Vero cell virogenesis Animalia Junin virus RNA viruses
spellingShingle	colchicine dimethyl sulfoxide egtazic acid nifedipine nocodazole octyl glucoside triton x 100 virus envelope protein virus glycoprotein virus protein animal cell article cytoskeleton Junin virus microfilament microtubule nonhuman priority journal Vero cell virogenesis Animalia Junin virus RNA viruses Candurra, Nélida Alicia Lago, María José Maskin, Laura Damonte, Elsa Beatriz Involvement of the cytoskeleton in Junin virus multiplication
topic_facet	colchicine dimethyl sulfoxide egtazic acid nifedipine nocodazole octyl glucoside triton x 100 virus envelope protein virus glycoprotein virus protein animal cell article cytoskeleton Junin virus microfilament microtubule nonhuman priority journal Vero cell virogenesis Animalia Junin virus RNA viruses
description	The role of the cellular cytoskeleton in Junin virus (JV) infection was explored in two ways. Firstly, the action of inhibitors that affect individual cytoskeletal systems (microtubules or microfilaments) selectively was analysed. It was found that perturbations of microtubule or microfilament networks caused by colchicine, nocodazole, nifedipine, EGTA or DMSO strongly affected virion production and viral protein expression at non-cytotoxic concentrations. Secondly, the extent of association of viral proteins and infectious virus particles with the cytoskeletal fraction of monkey Vero cells was determined by using three non-ionic detergents, Triton X-100 (TX-100), NP-40 and octyl glucoside (OG). The cytoskeleton retained nearly 70% of the external JV envelope glycoprotein GP38 and about 40% of the JV nucleoprotein NP, according to TX-100 and OG insolubility results. Furthermore, 1% of the total cell-bound infectivity was detected in the detergent-insoluble fraction, suggesting that cytoskeletal components are involved in the initiation of the assembly and budding processes of JV particles at the plasma membrane.
author	Candurra, Nélida Alicia Lago, María José Maskin, Laura Damonte, Elsa Beatriz
author_facet	Candurra, Nélida Alicia Lago, María José Maskin, Laura Damonte, Elsa Beatriz
author_sort	Candurra, Nélida Alicia
title	Involvement of the cytoskeleton in Junin virus multiplication
title_short	Involvement of the cytoskeleton in Junin virus multiplication
title_full	Involvement of the cytoskeleton in Junin virus multiplication
title_fullStr	Involvement of the cytoskeleton in Junin virus multiplication
title_full_unstemmed	Involvement of the cytoskeleton in Junin virus multiplication
title_sort	involvement of the cytoskeleton in junin virus multiplication
publishDate	1999
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v80_n1_p147_Candurra http://hdl.handle.net/20.500.12110/paper_00221317_v80_n1_p147_Candurra
work_keys_str_mv	AT candurranelidaalicia involvementofthecytoskeletoninjuninvirusmultiplication AT lagomariajose involvementofthecytoskeletoninjuninvirusmultiplication AT maskinlaura involvementofthecytoskeletoninjuninvirusmultiplication AT damonteelsabeatriz involvementofthecytoskeletoninjuninvirusmultiplication
_version_	1768546662110724096

Involvement of the cytoskeleton in Junin virus multiplication

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