Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.
Ethylene inhibits the establishment of symbiosis between rhizobia and legumes. Several rhizobia species express the enzyme ACC deaminase, which degrades the ethylene precursor 1-cyclopropane-1-carboxilate (ACC), leading to reductions in the amount of ethylene evolved by the plant. M. loti has a gene...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221260_v56_n4_p331_Conforte http://hdl.handle.net/20.500.12110/paper_00221260_v56_n4_p331_Conforte |
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paper:paper_00221260_v56_n4_p331_Conforte2023-06-08T14:46:29Z Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp. Conforte, Valeria Paola Echeverría, Mariela Ugalde, Rodolfo Augusto Menéndez, Ana Bernardina Lepek, Viviana Claudia ACC deaminase Competitiveness Ethylene Lotus Mesorhizobium loti 1 aminocyclopropanecarboxylic acid 1 aminocyclopropanecarboxylic acid deaminase deaminase ethylene unclassified drug 1-aminocyclopropane-1-carboxylate deaminase lyase article bacterial cell bacterium competence controlled study enzyme activity enzyme degradation forage genetic engineering legume Lotus Mesorhizobium loti nodulation nonhuman plant chromosome promoter region protein expression Rhizobiaceae strain difference structural gene symbiosis wild type Alphaproteobacteria enzymology genetics microbiology plant root symbiosis Lotus corniculatus var. japonicus Lotus tenuis Mesorhizobium loti Alphaproteobacteria Carbon-Carbon Lyases Lotus Plant Roots Symbiosis Ethylene inhibits the establishment of symbiosis between rhizobia and legumes. Several rhizobia species express the enzyme ACC deaminase, which degrades the ethylene precursor 1-cyclopropane-1-carboxilate (ACC), leading to reductions in the amount of ethylene evolved by the plant. M. loti has a gene encoding ACC deaminase, but this gene is under the activity of the NifA-RpoN- dependent promoter; thus, it is only expressed inside the nodule. The M. loti structural gene ACC deaminase (acdS) was integrated into the M. loti chromosome under a constitutive promoter activity. The resulting strain induced the formation of a higher number of nodules and was more competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results suggest that the introduction of the ACC deaminase activity within M. loti in a constitutive way could be a novel strategy to increase nodulation competitiveness of the bacteria, which could be useful for the forage inoculants industry. Fil:Conforte, V.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Echeverria, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ugalde, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Menéndez, A.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lepek, V.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221260_v56_n4_p331_Conforte http://hdl.handle.net/20.500.12110/paper_00221260_v56_n4_p331_Conforte |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
ACC deaminase Competitiveness Ethylene Lotus Mesorhizobium loti 1 aminocyclopropanecarboxylic acid 1 aminocyclopropanecarboxylic acid deaminase deaminase ethylene unclassified drug 1-aminocyclopropane-1-carboxylate deaminase lyase article bacterial cell bacterium competence controlled study enzyme activity enzyme degradation forage genetic engineering legume Lotus Mesorhizobium loti nodulation nonhuman plant chromosome promoter region protein expression Rhizobiaceae strain difference structural gene symbiosis wild type Alphaproteobacteria enzymology genetics microbiology plant root symbiosis Lotus corniculatus var. japonicus Lotus tenuis Mesorhizobium loti Alphaproteobacteria Carbon-Carbon Lyases Lotus Plant Roots Symbiosis |
| spellingShingle |
ACC deaminase Competitiveness Ethylene Lotus Mesorhizobium loti 1 aminocyclopropanecarboxylic acid 1 aminocyclopropanecarboxylic acid deaminase deaminase ethylene unclassified drug 1-aminocyclopropane-1-carboxylate deaminase lyase article bacterial cell bacterium competence controlled study enzyme activity enzyme degradation forage genetic engineering legume Lotus Mesorhizobium loti nodulation nonhuman plant chromosome promoter region protein expression Rhizobiaceae strain difference structural gene symbiosis wild type Alphaproteobacteria enzymology genetics microbiology plant root symbiosis Lotus corniculatus var. japonicus Lotus tenuis Mesorhizobium loti Alphaproteobacteria Carbon-Carbon Lyases Lotus Plant Roots Symbiosis Conforte, Valeria Paola Echeverría, Mariela Ugalde, Rodolfo Augusto Menéndez, Ana Bernardina Lepek, Viviana Claudia Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp. |
| topic_facet |
ACC deaminase Competitiveness Ethylene Lotus Mesorhizobium loti 1 aminocyclopropanecarboxylic acid 1 aminocyclopropanecarboxylic acid deaminase deaminase ethylene unclassified drug 1-aminocyclopropane-1-carboxylate deaminase lyase article bacterial cell bacterium competence controlled study enzyme activity enzyme degradation forage genetic engineering legume Lotus Mesorhizobium loti nodulation nonhuman plant chromosome promoter region protein expression Rhizobiaceae strain difference structural gene symbiosis wild type Alphaproteobacteria enzymology genetics microbiology plant root symbiosis Lotus corniculatus var. japonicus Lotus tenuis Mesorhizobium loti Alphaproteobacteria Carbon-Carbon Lyases Lotus Plant Roots Symbiosis |
| description |
Ethylene inhibits the establishment of symbiosis between rhizobia and legumes. Several rhizobia species express the enzyme ACC deaminase, which degrades the ethylene precursor 1-cyclopropane-1-carboxilate (ACC), leading to reductions in the amount of ethylene evolved by the plant. M. loti has a gene encoding ACC deaminase, but this gene is under the activity of the NifA-RpoN- dependent promoter; thus, it is only expressed inside the nodule. The M. loti structural gene ACC deaminase (acdS) was integrated into the M. loti chromosome under a constitutive promoter activity. The resulting strain induced the formation of a higher number of nodules and was more competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results suggest that the introduction of the ACC deaminase activity within M. loti in a constitutive way could be a novel strategy to increase nodulation competitiveness of the bacteria, which could be useful for the forage inoculants industry. |
| author |
Conforte, Valeria Paola Echeverría, Mariela Ugalde, Rodolfo Augusto Menéndez, Ana Bernardina Lepek, Viviana Claudia |
| author_facet |
Conforte, Valeria Paola Echeverría, Mariela Ugalde, Rodolfo Augusto Menéndez, Ana Bernardina Lepek, Viviana Claudia |
| author_sort |
Conforte, Valeria Paola |
| title |
Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp. |
| title_short |
Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp. |
| title_full |
Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp. |
| title_fullStr |
Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp. |
| title_full_unstemmed |
Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp. |
| title_sort |
engineered acc deaminase-expressing free-living cells of mesorhizobium loti show increased nodulation efficiency and competitiveness on lotus spp. |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221260_v56_n4_p331_Conforte http://hdl.handle.net/20.500.12110/paper_00221260_v56_n4_p331_Conforte |
| work_keys_str_mv |
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| _version_ |
1768544763910291456 |