Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea

Marine sponges are a rich source of structurally and biologically active metabolites of biomedical importance. We screened polar and non-polar samples of crude extracts obtained from marine sponges collected in different locations of Argentinean south sea coast, as a novel approach for their charact...

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Autores principales: Palermo, Jorge Alejandro, Rodríguez Brasco, María Florencia
Publicado: 2009
Materias:
Cell proliferation kinetics
Cytotoxicity
Genotoxicity
Marine sponges
Mitotic index
Sister chromatid exchange
animal product
biomarker
drug
genotoxicity
marine ecosystem
metabolite
sponge
toxicity test
variance analysis
Axinellidae
Callyspongia
Callyspongiidae
Chalinidae
Clathria
Cliona
Haliclona
Haliclona sp.
Halicnemia
Microciona
Microcionidae
Spongia
Spongiidae
Suberitidae
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220981_v369_n2_p144_Bartolotta
http://hdl.handle.net/20.500.12110/paper_00220981_v369_n2_p144_Bartolotta
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00220981_v369_n2_p144_Bartolotta
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spelling paper:paper_00220981_v369_n2_p144_Bartolotta2023-06-08T14:45:47Z Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea Palermo, Jorge Alejandro Rodríguez Brasco, María Florencia Cell proliferation kinetics Cytotoxicity Genotoxicity Marine sponges Mitotic index Sister chromatid exchange animal product biomarker drug genotoxicity marine ecosystem metabolite sponge toxicity test variance analysis Axinellidae Callyspongia Callyspongiidae Chalinidae Clathria Cliona Haliclona Haliclona sp. Halicnemia Microciona Microcionidae Spongia Spongiidae Suberitidae Marine sponges are a rich source of structurally and biologically active metabolites of biomedical importance. We screened polar and non-polar samples of crude extracts obtained from marine sponges collected in different locations of Argentinean south sea coast, as a novel approach for their characterization. The evaluation was performed using cytotoxic and genotoxic biomarkers such as mitotic index (MI), cell proliferation kinetics (CPK) and sister chromatid exchanges (SCE), monitored in vitro using peripheral blood lymphocytes. Statistical analysis was performed using two-way analysis of variance (ANOVA). The extracts evaluated belonged to: Callyspongia flabellata (BURTON, 1932) (Callyspongiidae); Plicatellopsis sp.(Suberitidae); Callyspongia fortis (RIDLEY, 1881) (Callyspongiidae); Clathria (Microciona) antarctica (TOPSENT, 1917) (Microcionidae); Spongia (Spongia) magellanica (THIELE, 1905) (Spongiidae); Halicnemia papillosa (THIELE, 1905) (Axinellidae); Cliona chilensis (THIELE, 1905) (Clionidae); Haliclona sp. 1; Haliclona sp. 2(Chalinidae). Genotoxicity studies revealed that the evaluated sponge extracts did not exhibit cytotoxic activity measured from mitotic index MI and cell proliferation kinetics(CPK). In contrast, sister chromatid exchanges (SCE) showed that the non-polar extract of Callyspongia fortis and the polar extract of Cliona chilensis presented significant differences in SCE frequency (p < 0.001), when compared with control cultures. These results emphasize the need to set up a standard battery of "in vitro" genotoxicity testing for new chemicals, pharmaceutical and drugs. © 2008 Elsevier B.V. All rights reserved. Fil:Palermo, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodriguez Brasco, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220981_v369_n2_p144_Bartolotta http://hdl.handle.net/20.500.12110/paper_00220981_v369_n2_p144_Bartolotta
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Cell proliferation kinetics
Cytotoxicity
Genotoxicity
Marine sponges
Mitotic index
Sister chromatid exchange
animal product
biomarker
drug
genotoxicity
marine ecosystem
metabolite
sponge
toxicity test
variance analysis
Axinellidae
Callyspongia
Callyspongiidae
Chalinidae
Clathria
Cliona
Haliclona
Haliclona sp.
Halicnemia
Microciona
Microcionidae
Spongia
Spongiidae
Suberitidae
spellingShingle Cell proliferation kinetics
Cytotoxicity
Genotoxicity
Marine sponges
Mitotic index
Sister chromatid exchange
animal product
biomarker
drug
genotoxicity
marine ecosystem
metabolite
sponge
toxicity test
variance analysis
Axinellidae
Callyspongia
Callyspongiidae
Chalinidae
Clathria
Cliona
Haliclona
Haliclona sp.
Halicnemia
Microciona
Microcionidae
Spongia
Spongiidae
Suberitidae
Palermo, Jorge Alejandro
Rodríguez Brasco, María Florencia
Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea
topic_facet Cell proliferation kinetics
Cytotoxicity
Genotoxicity
Marine sponges
Mitotic index
Sister chromatid exchange
animal product
biomarker
drug
genotoxicity
marine ecosystem
metabolite
sponge
toxicity test
variance analysis
Axinellidae
Callyspongia
Callyspongiidae
Chalinidae
Clathria
Cliona
Haliclona
Haliclona sp.
Halicnemia
Microciona
Microcionidae
Spongia
Spongiidae
Suberitidae
description Marine sponges are a rich source of structurally and biologically active metabolites of biomedical importance. We screened polar and non-polar samples of crude extracts obtained from marine sponges collected in different locations of Argentinean south sea coast, as a novel approach for their characterization. The evaluation was performed using cytotoxic and genotoxic biomarkers such as mitotic index (MI), cell proliferation kinetics (CPK) and sister chromatid exchanges (SCE), monitored in vitro using peripheral blood lymphocytes. Statistical analysis was performed using two-way analysis of variance (ANOVA). The extracts evaluated belonged to: Callyspongia flabellata (BURTON, 1932) (Callyspongiidae); Plicatellopsis sp.(Suberitidae); Callyspongia fortis (RIDLEY, 1881) (Callyspongiidae); Clathria (Microciona) antarctica (TOPSENT, 1917) (Microcionidae); Spongia (Spongia) magellanica (THIELE, 1905) (Spongiidae); Halicnemia papillosa (THIELE, 1905) (Axinellidae); Cliona chilensis (THIELE, 1905) (Clionidae); Haliclona sp. 1; Haliclona sp. 2(Chalinidae). Genotoxicity studies revealed that the evaluated sponge extracts did not exhibit cytotoxic activity measured from mitotic index MI and cell proliferation kinetics(CPK). In contrast, sister chromatid exchanges (SCE) showed that the non-polar extract of Callyspongia fortis and the polar extract of Cliona chilensis presented significant differences in SCE frequency (p < 0.001), when compared with control cultures. These results emphasize the need to set up a standard battery of "in vitro" genotoxicity testing for new chemicals, pharmaceutical and drugs. © 2008 Elsevier B.V. All rights reserved.
author Palermo, Jorge Alejandro
Rodríguez Brasco, María Florencia
author_facet Palermo, Jorge Alejandro
Rodríguez Brasco, María Florencia
author_sort Palermo, Jorge Alejandro
title Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea
title_short Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea
title_full Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea
title_fullStr Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea
title_full_unstemmed Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea
title_sort evaluation of genotoxic biomarkers in extracts of marine sponges from argentinean south sea
publishDate 2009
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220981_v369_n2_p144_Bartolotta
http://hdl.handle.net/20.500.12110/paper_00220981_v369_n2_p144_Bartolotta
work_keys_str_mv AT palermojorgealejandro evaluationofgenotoxicbiomarkersinextractsofmarinespongesfromargentineansouthsea
AT rodriguezbrascomariaflorencia evaluationofgenotoxicbiomarkersinextractsofmarinespongesfromargentineansouthsea
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