Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination

The role of GAs in promoting seed germination is well known and experiments with seeds from different species have suggested the requirement of de novo synthesis of GAs upon imbibition for germination. There are also strong indications that the enhancement of GA synthesis is part of the mechanism th...

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Autores principales:	Carrari, Fernando O., Rodríguez, María Verónica, Iusem, Norberto Daniel
Publicado:	2003
Materias:	Dormancy GA 20-oxidase Germination Gibberellins Sorghum Biosynthesis Enzymes Genes Tissue Seed germination Seed Amino Acid Sequence Blotting, Southern Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Mixed Function Oxygenases Molecular Sequence Data Plant Growth Regulators Poaceae RNA, Messenger Seeds Sequence Alignment Sequence Homology, Amino Acid Species Specificity Embryophyta Eukaryota
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v54_n390_p2071_PerezFlores http://hdl.handle.net/20.500.12110/paper_00220957_v54_n390_p2071_PerezFlores
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00220957_v54_n390_p2071_PerezFlores
record_format	dspace
spelling	paper:paper_00220957_v54_n390_p2071_PerezFlores2023-06-08T14:45:41Z Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination Carrari, Fernando O. Rodríguez, María Verónica Iusem, Norberto Daniel Dormancy GA 20-oxidase Germination Gibberellins Sorghum Biosynthesis Enzymes Genes Tissue Seed germination Seed Amino Acid Sequence Blotting, Southern Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Mixed Function Oxygenases Molecular Sequence Data Plant Growth Regulators Poaceae RNA, Messenger Seeds Sequence Alignment Sequence Homology, Amino Acid Species Specificity Embryophyta Eukaryota The role of GAs in promoting seed germination is well known and experiments with seeds from different species have suggested the requirement of de novo synthesis of GAs upon imbibition for germination. There are also strong indications that the enhancement of GA synthesis is part of the mechanism through which environmental signals (i.e. light) induce germination. Since along the GA biosynthetic pathway, oxidation at C-20 carried out by GA 20-oxidases is thought to be a site of regulation, a cDNA clone encoding a GA 20-oxidase was isolated from embryos of sorghum (SbGA 20ox). Expression analysis of this gene in embryos within imbibed caryopses with low dormancy showed detectable amounts of the specific mRNA early upon incubation, increasing thereafter. In contrast, it remained barely detectable in embryos from dormant caryopses. Changes in endogenous GA4 levels were in agreement with those of SbGA 20ox mRNA, suggesting that GA production might be regulated differentially at the level of transcription of this gene. The expression of SbGA 20ox was enhanced in incubated embryos isolated from either type of caryopses, illustrating a physiological control exerted by the surrounding seed tissues on gene expression. The results also show that ABA leads to a suppression of transcription of this gene. Fil:Carrari, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v54_n390_p2071_PerezFlores http://hdl.handle.net/20.500.12110/paper_00220957_v54_n390_p2071_PerezFlores
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Dormancy GA 20-oxidase Germination Gibberellins Sorghum Biosynthesis Enzymes Genes Tissue Seed germination Seed Amino Acid Sequence Blotting, Southern Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Mixed Function Oxygenases Molecular Sequence Data Plant Growth Regulators Poaceae RNA, Messenger Seeds Sequence Alignment Sequence Homology, Amino Acid Species Specificity Embryophyta Eukaryota
spellingShingle	Dormancy GA 20-oxidase Germination Gibberellins Sorghum Biosynthesis Enzymes Genes Tissue Seed germination Seed Amino Acid Sequence Blotting, Southern Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Mixed Function Oxygenases Molecular Sequence Data Plant Growth Regulators Poaceae RNA, Messenger Seeds Sequence Alignment Sequence Homology, Amino Acid Species Specificity Embryophyta Eukaryota Carrari, Fernando O. Rodríguez, María Verónica Iusem, Norberto Daniel Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination
topic_facet	Dormancy GA 20-oxidase Germination Gibberellins Sorghum Biosynthesis Enzymes Genes Tissue Seed germination Seed Amino Acid Sequence Blotting, Southern Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Mixed Function Oxygenases Molecular Sequence Data Plant Growth Regulators Poaceae RNA, Messenger Seeds Sequence Alignment Sequence Homology, Amino Acid Species Specificity Embryophyta Eukaryota
description	The role of GAs in promoting seed germination is well known and experiments with seeds from different species have suggested the requirement of de novo synthesis of GAs upon imbibition for germination. There are also strong indications that the enhancement of GA synthesis is part of the mechanism through which environmental signals (i.e. light) induce germination. Since along the GA biosynthetic pathway, oxidation at C-20 carried out by GA 20-oxidases is thought to be a site of regulation, a cDNA clone encoding a GA 20-oxidase was isolated from embryos of sorghum (SbGA 20ox). Expression analysis of this gene in embryos within imbibed caryopses with low dormancy showed detectable amounts of the specific mRNA early upon incubation, increasing thereafter. In contrast, it remained barely detectable in embryos from dormant caryopses. Changes in endogenous GA4 levels were in agreement with those of SbGA 20ox mRNA, suggesting that GA production might be regulated differentially at the level of transcription of this gene. The expression of SbGA 20ox was enhanced in incubated embryos isolated from either type of caryopses, illustrating a physiological control exerted by the surrounding seed tissues on gene expression. The results also show that ABA leads to a suppression of transcription of this gene.
author	Carrari, Fernando O. Rodríguez, María Verónica Iusem, Norberto Daniel
author_facet	Carrari, Fernando O. Rodríguez, María Verónica Iusem, Norberto Daniel
author_sort	Carrari, Fernando O.
title	Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination
title_short	Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination
title_full	Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination
title_fullStr	Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination
title_full_unstemmed	Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination
title_sort	expression analysis of a ga 20-oxidase in embryos from two sorghum lines with contrasting dormancy: possible participation of this gene in the hormonal control of germination
publishDate	2003
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v54_n390_p2071_PerezFlores http://hdl.handle.net/20.500.12110/paper_00220957_v54_n390_p2071_PerezFlores
work_keys_str_mv	AT carrarifernandoo expressionanalysisofaga20oxidaseinembryosfromtwosorghumlineswithcontrastingdormancypossibleparticipationofthisgeneinthehormonalcontrolofgermination AT rodriguezmariaveronica expressionanalysisofaga20oxidaseinembryosfromtwosorghumlineswithcontrastingdormancypossibleparticipationofthisgeneinthehormonalcontrolofgermination AT iusemnorbertodaniel expressionanalysisofaga20oxidaseinembryosfromtwosorghumlineswithcontrastingdormancypossibleparticipationofthisgeneinthehormonalcontrolofgermination
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Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination

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