Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america
Protoplasts were successfully isolated from internodal callus tissues of both Oxalis glaucifolia and O. rhombeo-ovata when they were digested in a solution containing 0.1% (w/v) Macerozyme R-10, 0.5% (w/v) cellulase Onozuka R-10 and 0.3 mmol m-3 sucrose. Protoplasts proliferated to give cell colonie...
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Publicado: |
1989
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v40_n4_p493_Ochatt http://hdl.handle.net/20.500.12110/paper_00220957_v40_n4_p493_Ochatt |
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