Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america

Protoplasts were successfully isolated from internodal callus tissues of both Oxalis glaucifolia and O. rhombeo-ovata when they were digested in a solution containing 0.1% (w/v) Macerozyme R-10, 0.5% (w/v) cellulase Onozuka R-10 and 0.3 mmol m-3 sucrose. Protoplasts proliferated to give cell colonie...

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Guardado en:
Detalles Bibliográficos
Publicado: 1989
Materias:
Oxalis spp
Plant regeneration
Protoplasts
Tissue culture
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v40_n4_p493_Ochatt
http://hdl.handle.net/20.500.12110/paper_00220957_v40_n4_p493_Ochatt
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00220957_v40_n4_p493_Ochatt
record_format dspace
spelling paper:paper_00220957_v40_n4_p493_Ochatt2023-06-08T14:45:41Z Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america Oxalis spp Plant regeneration Protoplasts Tissue culture Protoplasts were successfully isolated from internodal callus tissues of both Oxalis glaucifolia and O. rhombeo-ovata when they were digested in a solution containing 0.1% (w/v) Macerozyme R-10, 0.5% (w/v) cellulase Onozuka R-10 and 0.3 mmol m-3 sucrose. Protoplasts proliferated to give cell colonies on Gamborg et al.'s B5 medium supplemented with 0.3 mmol m-3 mannitol, 0.5 mg dm-32, 4-D, and 2.0 mg dm-3 kinetin. Callus was produced upon transfer of cell colonies to Murashige and Skoog medium containing 2.0 mg dm-3 l-naphthaleneacetic acid (NAA) and 0.1 mg dm-3 kinetin for O. glaucifolia, or with 5.0 mg dm-3 NAA and 0.5 mg dm-3 6-benzylaminopurine, for O. rhombeo-ovata. Plants were regenerated from O. glaucifolia protoplasts on a medium containing 0.1 mg dm-3 NAA, 1.0 mg dm-3 kinetin and 1.0 mg dm-3 gibberellic acid, but only vascular nodules were differentiated by O. rhombeo-ovata protoplast-derived calli. © 1989 Oxford University Press. 1989 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v40_n4_p493_Ochatt http://hdl.handle.net/20.500.12110/paper_00220957_v40_n4_p493_Ochatt
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Oxalis spp
Plant regeneration
Protoplasts
Tissue culture
spellingShingle Oxalis spp
Plant regeneration
Protoplasts
Tissue culture
Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america
topic_facet Oxalis spp
Plant regeneration
Protoplasts
Tissue culture
description Protoplasts were successfully isolated from internodal callus tissues of both Oxalis glaucifolia and O. rhombeo-ovata when they were digested in a solution containing 0.1% (w/v) Macerozyme R-10, 0.5% (w/v) cellulase Onozuka R-10 and 0.3 mmol m-3 sucrose. Protoplasts proliferated to give cell colonies on Gamborg et al.'s B5 medium supplemented with 0.3 mmol m-3 mannitol, 0.5 mg dm-32, 4-D, and 2.0 mg dm-3 kinetin. Callus was produced upon transfer of cell colonies to Murashige and Skoog medium containing 2.0 mg dm-3 l-naphthaleneacetic acid (NAA) and 0.1 mg dm-3 kinetin for O. glaucifolia, or with 5.0 mg dm-3 NAA and 0.5 mg dm-3 6-benzylaminopurine, for O. rhombeo-ovata. Plants were regenerated from O. glaucifolia protoplasts on a medium containing 0.1 mg dm-3 NAA, 1.0 mg dm-3 kinetin and 1.0 mg dm-3 gibberellic acid, but only vascular nodules were differentiated by O. rhombeo-ovata protoplast-derived calli. © 1989 Oxford University Press.
title Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america
title_short Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america
title_full Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america
title_fullStr Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america
title_full_unstemmed Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america
title_sort isolation, culture, and plant regeneration of protoplasts of two shrubby oxalis species from south america
publishDate 1989
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v40_n4_p493_Ochatt
http://hdl.handle.net/20.500.12110/paper_00220957_v40_n4_p493_Ochatt
_version_ 1768544763357691904

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