Heat-stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines of Drosophila melanogaster
In insects, pre-adult stages of the life cycle are exposed to variation in temperature that may differ from that in adults. However, the genetic basis for adaptation to environmental temperature could be similar between the pre-adult and the adult stages of the life cycle. Here, we tested quantitati...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v216_n15_p2953_Sambucetti http://hdl.handle.net/20.500.12110/paper_00220949_v216_n15_p2953_Sambucetti |
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paper:paper_00220949_v216_n15_p2953_Sambucetti2023-06-08T14:45:37Z Heat-stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines of Drosophila melanogaster Candidate genes Heat shock Larval survival QTL Thermotolerance analysis of variance animal article candidate genes Drosophila melanogaster female genetic recombination genetics growth, development and aging heat shock heat shock response heat tolerance inbreeding larval survival life cycle stage male physiology quantitative trait locus statistical model candidate genes heat shock larval survival QTL thermotolerance Analysis of Variance Animals Drosophila melanogaster Female Heat-Shock Response Inbreeding Life Cycle Stages Likelihood Functions Male Quantitative Trait Loci Recombination, Genetic In insects, pre-adult stages of the life cycle are exposed to variation in temperature that may differ from that in adults. However, the genetic basis for adaptation to environmental temperature could be similar between the pre-adult and the adult stages of the life cycle. Here, we tested quantitative trait loci (QTL) for heat-stress survival in larvae of Drosophila melanogaster, with and without a mild-heat-stress pre-treatment. Two sets of recombinant inbred lines derived from lines artificially selected for high and low levels of knockdown resistance to high temperature in young flies were used as the mapping population. There was no apparent increase in heat-shock survival between heat-pretreated and non-pretreated larvae. There was a positive correlation between the two experimental conditions of heat-shock survival (with and without a heat pre-treatment) except for males from one set of lines. Several QTL were identified involving all three major chromosomes. Most QTL for larval thermotolerance overlapped with thermotolerance QTL identified in previous studies for adults, indicating that heat-stress resistance is not genetically independent between life cycle stages because of either linkage or pleiotropy. The sign of the effects of some QTL alleles differed both between the sexes and between life stages. © 2013. Published by The Company of Biologists Ltd. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v216_n15_p2953_Sambucetti http://hdl.handle.net/20.500.12110/paper_00220949_v216_n15_p2953_Sambucetti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Candidate genes Heat shock Larval survival QTL Thermotolerance analysis of variance animal article candidate genes Drosophila melanogaster female genetic recombination genetics growth, development and aging heat shock heat shock response heat tolerance inbreeding larval survival life cycle stage male physiology quantitative trait locus statistical model candidate genes heat shock larval survival QTL thermotolerance Analysis of Variance Animals Drosophila melanogaster Female Heat-Shock Response Inbreeding Life Cycle Stages Likelihood Functions Male Quantitative Trait Loci Recombination, Genetic |
| spellingShingle |
Candidate genes Heat shock Larval survival QTL Thermotolerance analysis of variance animal article candidate genes Drosophila melanogaster female genetic recombination genetics growth, development and aging heat shock heat shock response heat tolerance inbreeding larval survival life cycle stage male physiology quantitative trait locus statistical model candidate genes heat shock larval survival QTL thermotolerance Analysis of Variance Animals Drosophila melanogaster Female Heat-Shock Response Inbreeding Life Cycle Stages Likelihood Functions Male Quantitative Trait Loci Recombination, Genetic Heat-stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines of Drosophila melanogaster |
| topic_facet |
Candidate genes Heat shock Larval survival QTL Thermotolerance analysis of variance animal article candidate genes Drosophila melanogaster female genetic recombination genetics growth, development and aging heat shock heat shock response heat tolerance inbreeding larval survival life cycle stage male physiology quantitative trait locus statistical model candidate genes heat shock larval survival QTL thermotolerance Analysis of Variance Animals Drosophila melanogaster Female Heat-Shock Response Inbreeding Life Cycle Stages Likelihood Functions Male Quantitative Trait Loci Recombination, Genetic |
| description |
In insects, pre-adult stages of the life cycle are exposed to variation in temperature that may differ from that in adults. However, the genetic basis for adaptation to environmental temperature could be similar between the pre-adult and the adult stages of the life cycle. Here, we tested quantitative trait loci (QTL) for heat-stress survival in larvae of Drosophila melanogaster, with and without a mild-heat-stress pre-treatment. Two sets of recombinant inbred lines derived from lines artificially selected for high and low levels of knockdown resistance to high temperature in young flies were used as the mapping population. There was no apparent increase in heat-shock survival between heat-pretreated and non-pretreated larvae. There was a positive correlation between the two experimental conditions of heat-shock survival (with and without a heat pre-treatment) except for males from one set of lines. Several QTL were identified involving all three major chromosomes. Most QTL for larval thermotolerance overlapped with thermotolerance QTL identified in previous studies for adults, indicating that heat-stress resistance is not genetically independent between life cycle stages because of either linkage or pleiotropy. The sign of the effects of some QTL alleles differed both between the sexes and between life stages. © 2013. Published by The Company of Biologists Ltd. |
| title |
Heat-stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines of Drosophila melanogaster |
| title_short |
Heat-stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines of Drosophila melanogaster |
| title_full |
Heat-stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines of Drosophila melanogaster |
| title_fullStr |
Heat-stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines of Drosophila melanogaster |
| title_full_unstemmed |
Heat-stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines of Drosophila melanogaster |
| title_sort |
heat-stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines of drosophila melanogaster |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v216_n15_p2953_Sambucetti http://hdl.handle.net/20.500.12110/paper_00220949_v216_n15_p2953_Sambucetti |
| _version_ |
1768544896945225728 |