Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria
Bovine mastitis is one of the most economically deleterious diseases affecting dairy herds and results from an infection of the udder by pathogenic microorganisms such as Staphylococcus aureus, Streptococcus uberis, and Escherichia coli. The mammary gland is capable of preventing and combating bacte...
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2011
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220302_v94_n1_p138_Chaneton http://hdl.handle.net/20.500.12110/paper_00220302_v94_n1_p138_Chaneton |
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paper:paper_00220302_v94_n1_p138_Chaneton2023-06-08T14:45:06Z Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria β-lactoglobulin Antimicrobial Mastitis-causing bacteria antiinfective agent lactoglobulin animal animal disease article bacterial count bovine mastitis cattle classification drug effect Escherichia coli female genetics growth, development and aging microbiology Staphylococcus aureus Streptococcus Animals Anti-Infective Agents Cattle Colony Count, Microbial Escherichia coli Female Lactoglobulins Mastitis, Bovine Staphylococcus aureus Streptococcus Bacteria (microorganisms) Bovinae Escherichia coli Lagomorpha Mammalia Rodentia Staphylococcus aureus Streptococcus uberis Bovine mastitis is one of the most economically deleterious diseases affecting dairy herds and results from an infection of the udder by pathogenic microorganisms such as Staphylococcus aureus, Streptococcus uberis, and Escherichia coli. The mammary gland is capable of preventing and combating bacterial infection by means of a complex network of innate and adaptive immune mechanisms. Lactoferrin is an 86-kDa protein with antibacterial activity that plays a role in the mammary gland's defense against infection. β-Lactoglobulin (β-LG) is an 18-kDa protein that is present in most mammals but is notably absent in humans, rodents, and lagomorphs. Different genetic variants of this protein exist, with β-LG A and β-LG B being the most common. In spite of being well studied, the biological function of β-LG is not thoroughly understood, and most noticeably, no reports exist on the effects of the native protein on bacterial growth. Hence, the objective of this study was to assess the potential antibacterial activity of β-LG against mastitis agents. To do this, we purified β-LG from normal bovine milk using a mild, nondenaturing method and performed in vitro growth inhibition assays with Staph. aureus, E. coli, and Strep. uberis. β-Lactoglobulin inhibited the growth of Staph. aureus and Strep. uberis but had no effect on E. coli. The antimicrobial activity against Staph. aureus and Strep. uberis was concentration dependent and was elicited by the intact protein because Tricine-sodium dodecyl sulfate-PAGE and analytical gel filtration chromatography did not reveal the presence of short degradation peptides. Analysis of the genetic variants of β-LG showed that β-LG A has higher inhibitory activity against Staph. aureus and Strep. uberis than β-LG B. Coincubation of β-LG and lactoferrin resulted in an augmented antibacterial activity against Staph. aureus, suggesting an additive effect of the proteins. This result, along with the proteins' complementary spectrum of action, suggests that β-LG and lactoferrin may complement each other in the mammary gland's defenses against bacterial infection. © 2011 American Dairy Science Association. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220302_v94_n1_p138_Chaneton http://hdl.handle.net/20.500.12110/paper_00220302_v94_n1_p138_Chaneton |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
β-lactoglobulin Antimicrobial Mastitis-causing bacteria antiinfective agent lactoglobulin animal animal disease article bacterial count bovine mastitis cattle classification drug effect Escherichia coli female genetics growth, development and aging microbiology Staphylococcus aureus Streptococcus Animals Anti-Infective Agents Cattle Colony Count, Microbial Escherichia coli Female Lactoglobulins Mastitis, Bovine Staphylococcus aureus Streptococcus Bacteria (microorganisms) Bovinae Escherichia coli Lagomorpha Mammalia Rodentia Staphylococcus aureus Streptococcus uberis |
| spellingShingle |
β-lactoglobulin Antimicrobial Mastitis-causing bacteria antiinfective agent lactoglobulin animal animal disease article bacterial count bovine mastitis cattle classification drug effect Escherichia coli female genetics growth, development and aging microbiology Staphylococcus aureus Streptococcus Animals Anti-Infective Agents Cattle Colony Count, Microbial Escherichia coli Female Lactoglobulins Mastitis, Bovine Staphylococcus aureus Streptococcus Bacteria (microorganisms) Bovinae Escherichia coli Lagomorpha Mammalia Rodentia Staphylococcus aureus Streptococcus uberis Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria |
| topic_facet |
β-lactoglobulin Antimicrobial Mastitis-causing bacteria antiinfective agent lactoglobulin animal animal disease article bacterial count bovine mastitis cattle classification drug effect Escherichia coli female genetics growth, development and aging microbiology Staphylococcus aureus Streptococcus Animals Anti-Infective Agents Cattle Colony Count, Microbial Escherichia coli Female Lactoglobulins Mastitis, Bovine Staphylococcus aureus Streptococcus Bacteria (microorganisms) Bovinae Escherichia coli Lagomorpha Mammalia Rodentia Staphylococcus aureus Streptococcus uberis |
| description |
Bovine mastitis is one of the most economically deleterious diseases affecting dairy herds and results from an infection of the udder by pathogenic microorganisms such as Staphylococcus aureus, Streptococcus uberis, and Escherichia coli. The mammary gland is capable of preventing and combating bacterial infection by means of a complex network of innate and adaptive immune mechanisms. Lactoferrin is an 86-kDa protein with antibacterial activity that plays a role in the mammary gland's defense against infection. β-Lactoglobulin (β-LG) is an 18-kDa protein that is present in most mammals but is notably absent in humans, rodents, and lagomorphs. Different genetic variants of this protein exist, with β-LG A and β-LG B being the most common. In spite of being well studied, the biological function of β-LG is not thoroughly understood, and most noticeably, no reports exist on the effects of the native protein on bacterial growth. Hence, the objective of this study was to assess the potential antibacterial activity of β-LG against mastitis agents. To do this, we purified β-LG from normal bovine milk using a mild, nondenaturing method and performed in vitro growth inhibition assays with Staph. aureus, E. coli, and Strep. uberis. β-Lactoglobulin inhibited the growth of Staph. aureus and Strep. uberis but had no effect on E. coli. The antimicrobial activity against Staph. aureus and Strep. uberis was concentration dependent and was elicited by the intact protein because Tricine-sodium dodecyl sulfate-PAGE and analytical gel filtration chromatography did not reveal the presence of short degradation peptides. Analysis of the genetic variants of β-LG showed that β-LG A has higher inhibitory activity against Staph. aureus and Strep. uberis than β-LG B. Coincubation of β-LG and lactoferrin resulted in an augmented antibacterial activity against Staph. aureus, suggesting an additive effect of the proteins. This result, along with the proteins' complementary spectrum of action, suggests that β-LG and lactoferrin may complement each other in the mammary gland's defenses against bacterial infection. © 2011 American Dairy Science Association. |
| title |
Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria |
| title_short |
Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria |
| title_full |
Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria |
| title_fullStr |
Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria |
| title_full_unstemmed |
Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria |
| title_sort |
antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria |
| publishDate |
2011 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220302_v94_n1_p138_Chaneton http://hdl.handle.net/20.500.12110/paper_00220302_v94_n1_p138_Chaneton |
| _version_ |
1768542252913655808 |