Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections
Few studies have described the relationship between genotypic and phenotypic methods for detecting penicillin resistance in Staphylococcus aureus isolated from bovine intramammary infection (IMI). Six phenotypic methods for penicillinase detection were compared with a genotypic method testing the pr...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220299_v82_n3_p317_Russi http://hdl.handle.net/20.500.12110/paper_00220299_v82_n3_p317_Russi |
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paper:paper_00220299_v82_n3_p317_Russi2023-06-08T14:45:06Z Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections blaZ intramammary infections penicillin resistance Staphylococcus aureus Bovinae Staphylococcus aureus bacterial DNA beta lactamase penicillin G penicillinase animal bovine bovine mastitis comparative study drug effects enzymology female genetics genotype laboratory diagnosis microbial sensitivity test microbiology penicillin resistance phenotype Staphylococcus aureus Staphylococcus infection veterinary Animals beta-Lactamases Cattle DNA, Bacterial False Negative Reactions Female Genotype Mastitis, Bovine Microbial Sensitivity Tests Penicillin G Penicillin Resistance Penicillinase Phenotype Staphylococcal Infections Staphylococcus aureus Few studies have described the relationship between genotypic and phenotypic methods for detecting penicillin resistance in Staphylococcus aureus isolated from bovine intramammary infection (IMI). Six phenotypic methods for penicillinase detection were compared with a genotypic method testing the presence of the β-lactamase gene blaZ in Staph. aureus (n = 150) isolated from bovine IMI. Highest sensitivities and specificities were observed for disk diffusion (DD) (93 and 97·4%), minimum inhibitory concentration (MIC) (90·3 and 97·4%), Cefinase™ (85·9 and 97·4%) and Diatabs™ (85·7 and 98·7%). The estimated cut-off points estimated in the present study can be considered close to the ones indicated by CLSI (2013). The molecular detection of blaZ gene is the only method that may indicate the real or potential capacity of producing β-lactamase in Staph. aureus. Considering that from a clinical standpoint a false negative result from a phenotypic test is the most unfavourable situation, a combination of standard DD with Diatabs™ or Cefinase™ should be performed by routine mastitis laboratories to minimise false negative results. © 2015 Proprietors of Journal of Dairy Research. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220299_v82_n3_p317_Russi http://hdl.handle.net/20.500.12110/paper_00220299_v82_n3_p317_Russi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
blaZ intramammary infections penicillin resistance Staphylococcus aureus Bovinae Staphylococcus aureus bacterial DNA beta lactamase penicillin G penicillinase animal bovine bovine mastitis comparative study drug effects enzymology female genetics genotype laboratory diagnosis microbial sensitivity test microbiology penicillin resistance phenotype Staphylococcus aureus Staphylococcus infection veterinary Animals beta-Lactamases Cattle DNA, Bacterial False Negative Reactions Female Genotype Mastitis, Bovine Microbial Sensitivity Tests Penicillin G Penicillin Resistance Penicillinase Phenotype Staphylococcal Infections Staphylococcus aureus |
| spellingShingle |
blaZ intramammary infections penicillin resistance Staphylococcus aureus Bovinae Staphylococcus aureus bacterial DNA beta lactamase penicillin G penicillinase animal bovine bovine mastitis comparative study drug effects enzymology female genetics genotype laboratory diagnosis microbial sensitivity test microbiology penicillin resistance phenotype Staphylococcus aureus Staphylococcus infection veterinary Animals beta-Lactamases Cattle DNA, Bacterial False Negative Reactions Female Genotype Mastitis, Bovine Microbial Sensitivity Tests Penicillin G Penicillin Resistance Penicillinase Phenotype Staphylococcal Infections Staphylococcus aureus Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections |
| topic_facet |
blaZ intramammary infections penicillin resistance Staphylococcus aureus Bovinae Staphylococcus aureus bacterial DNA beta lactamase penicillin G penicillinase animal bovine bovine mastitis comparative study drug effects enzymology female genetics genotype laboratory diagnosis microbial sensitivity test microbiology penicillin resistance phenotype Staphylococcus aureus Staphylococcus infection veterinary Animals beta-Lactamases Cattle DNA, Bacterial False Negative Reactions Female Genotype Mastitis, Bovine Microbial Sensitivity Tests Penicillin G Penicillin Resistance Penicillinase Phenotype Staphylococcal Infections Staphylococcus aureus |
| description |
Few studies have described the relationship between genotypic and phenotypic methods for detecting penicillin resistance in Staphylococcus aureus isolated from bovine intramammary infection (IMI). Six phenotypic methods for penicillinase detection were compared with a genotypic method testing the presence of the β-lactamase gene blaZ in Staph. aureus (n = 150) isolated from bovine IMI. Highest sensitivities and specificities were observed for disk diffusion (DD) (93 and 97·4%), minimum inhibitory concentration (MIC) (90·3 and 97·4%), Cefinase™ (85·9 and 97·4%) and Diatabs™ (85·7 and 98·7%). The estimated cut-off points estimated in the present study can be considered close to the ones indicated by CLSI (2013). The molecular detection of blaZ gene is the only method that may indicate the real or potential capacity of producing β-lactamase in Staph. aureus. Considering that from a clinical standpoint a false negative result from a phenotypic test is the most unfavourable situation, a combination of standard DD with Diatabs™ or Cefinase™ should be performed by routine mastitis laboratories to minimise false negative results. © 2015 Proprietors of Journal of Dairy Research. |
| title |
Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections |
| title_short |
Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections |
| title_full |
Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections |
| title_fullStr |
Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections |
| title_full_unstemmed |
Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections |
| title_sort |
comparison of phenotypic tests for detecting penicillin g resistance with presence of blaz gene in staphylococcus aureus isolated from bovine intramammary infections |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220299_v82_n3_p317_Russi http://hdl.handle.net/20.500.12110/paper_00220299_v82_n3_p317_Russi |
| _version_ |
1768544212025868288 |