Absorption and scattering coefficients: A biophysical-chemistry experiment using reflectance spectroscopy

Knowledge of absorption and scattering coefficients is relevant to different areas such as for the prediction of colors. They are also important for leaves, where they are connected with photon transport and photosynthesis. An experiment was conducted to calculate the absorption and scattering coeff...

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Detalles Bibliográficos
Autores principales:	Cordon, Gabriela Beatriz, Lagorio, María Gabriela
Publicado:	2007
Materias:	Absorption Biophysics Chlorophyll Near infrared spectroscopy Photons Photosynthesis Reflection Scattering Biophysical-chemistry experiment Hedera helix Kubelka-Munk theory Hardwoods Carotenoids Chlorophylls Citrus Diffuse Reflection Ficus Gardenia Leaves Ligustrum
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219584_v84_n7_p1167_Cordon http://hdl.handle.net/20.500.12110/paper_00219584_v84_n7_p1167_Cordon
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00219584_v84_n7_p1167_Cordon
record_format	dspace
spelling	paper:paper_00219584_v84_n7_p1167_Cordon2025-07-30T17:26:15Z Absorption and scattering coefficients: A biophysical-chemistry experiment using reflectance spectroscopy Cordon, Gabriela Beatriz Lagorio, María Gabriela Absorption Biophysics Chlorophyll Near infrared spectroscopy Photons Photosynthesis Reflection Scattering Biophysical-chemistry experiment Hedera helix Kubelka-Munk theory Hardwoods Absorption Carotenoids Chlorophylls Citrus Diffuse Reflection Ficus Gardenia Leaves Ligustrum Photosynthesis Reflection Scattering Knowledge of absorption and scattering coefficients is relevant to different areas such as for the prediction of colors. They are also important for leaves, where they are connected with photon transport and photosynthesis. An experiment was conducted to calculate the absorption and scattering coefficients on leaves. Leaves from the species Citrus aurantium, Ficus benjamina, Gardenia jasminoides, Hedera helix and Ligustrum vulgare were used. Diffuse reflectance was recorded as a function of wave-length from 450-700 nm for individual leaves and from groups of eight stacked leaves and diffuse transmittance. Results show that near infrared reflectance of leaves is determined by their structure while middle infrared reflectance is determined by the water content of the leaves. However, between 400-700 nm, reflectance is mainly determined by the absorption of the photosynthetic pigments. The absorption is due to chlorophylls a and b for wavelengths longer than 550 nm. For shorter ones, the absorption is determined mainly by chlorophylls a, b and carotenoids. The experiment also showed that the method used, the Kubelka-Munk theory and the model of the pile of plates, is adequate for calculating absorption and scattering coefficients of leaves. Fil:Cordon, G.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lagorio, M.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219584_v84_n7_p1167_Cordon http://hdl.handle.net/20.500.12110/paper_00219584_v84_n7_p1167_Cordon
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Absorption Biophysics Chlorophyll Near infrared spectroscopy Photons Photosynthesis Reflection Scattering Biophysical-chemistry experiment Hedera helix Kubelka-Munk theory Hardwoods Absorption Carotenoids Chlorophylls Citrus Diffuse Reflection Ficus Gardenia Leaves Ligustrum Photosynthesis Reflection Scattering
spellingShingle	Absorption Biophysics Chlorophyll Near infrared spectroscopy Photons Photosynthesis Reflection Scattering Biophysical-chemistry experiment Hedera helix Kubelka-Munk theory Hardwoods Absorption Carotenoids Chlorophylls Citrus Diffuse Reflection Ficus Gardenia Leaves Ligustrum Photosynthesis Reflection Scattering Cordon, Gabriela Beatriz Lagorio, María Gabriela Absorption and scattering coefficients: A biophysical-chemistry experiment using reflectance spectroscopy
topic_facet	Absorption Biophysics Chlorophyll Near infrared spectroscopy Photons Photosynthesis Reflection Scattering Biophysical-chemistry experiment Hedera helix Kubelka-Munk theory Hardwoods Absorption Carotenoids Chlorophylls Citrus Diffuse Reflection Ficus Gardenia Leaves Ligustrum Photosynthesis Reflection Scattering
description	Knowledge of absorption and scattering coefficients is relevant to different areas such as for the prediction of colors. They are also important for leaves, where they are connected with photon transport and photosynthesis. An experiment was conducted to calculate the absorption and scattering coefficients on leaves. Leaves from the species Citrus aurantium, Ficus benjamina, Gardenia jasminoides, Hedera helix and Ligustrum vulgare were used. Diffuse reflectance was recorded as a function of wave-length from 450-700 nm for individual leaves and from groups of eight stacked leaves and diffuse transmittance. Results show that near infrared reflectance of leaves is determined by their structure while middle infrared reflectance is determined by the water content of the leaves. However, between 400-700 nm, reflectance is mainly determined by the absorption of the photosynthetic pigments. The absorption is due to chlorophylls a and b for wavelengths longer than 550 nm. For shorter ones, the absorption is determined mainly by chlorophylls a, b and carotenoids. The experiment also showed that the method used, the Kubelka-Munk theory and the model of the pile of plates, is adequate for calculating absorption and scattering coefficients of leaves.
author	Cordon, Gabriela Beatriz Lagorio, María Gabriela
author_facet	Cordon, Gabriela Beatriz Lagorio, María Gabriela
author_sort	Cordon, Gabriela Beatriz
title	Absorption and scattering coefficients: A biophysical-chemistry experiment using reflectance spectroscopy
title_short	Absorption and scattering coefficients: A biophysical-chemistry experiment using reflectance spectroscopy
title_full	Absorption and scattering coefficients: A biophysical-chemistry experiment using reflectance spectroscopy
title_fullStr	Absorption and scattering coefficients: A biophysical-chemistry experiment using reflectance spectroscopy
title_full_unstemmed	Absorption and scattering coefficients: A biophysical-chemistry experiment using reflectance spectroscopy
title_sort	absorption and scattering coefficients: a biophysical-chemistry experiment using reflectance spectroscopy
publishDate	2007
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219584_v84_n7_p1167_Cordon http://hdl.handle.net/20.500.12110/paper_00219584_v84_n7_p1167_Cordon
work_keys_str_mv	AT cordongabrielabeatriz absorptionandscatteringcoefficientsabiophysicalchemistryexperimentusingreflectancespectroscopy AT lagoriomariagabriela absorptionandscatteringcoefficientsabiophysicalchemistryexperimentusingreflectancespectroscopy
_version_	1840325437645914112

Absorption and scattering coefficients: A biophysical-chemistry experiment using reflectance spectroscopy

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