Anti-tumor effect of SLPI on mammary but not colon tumor growth

Secretory leukocyte protease inhibitor (SLPI) is a serine protease inhibitor that was related to cancer development and metastasis dissemination on several types of tumors. However, it is not known the effect of SLPI on mammary and colon tumors. The aim of this study was to examine the effect of SLP...

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Detalles Bibliográficos
Autores principales:	Amiano, Nicolás Oscar, Reiteri, Romina Macarena, Guerrieri, Diego, Orqueda, Andrés Javier
Publicado:	2013
Materias:	caspase 3 secretory leukocyte proteinase inhibitor small interfering RNA animal cell animal experiment animal model antineoplastic activity apoptosis article breast adenocarcinoma breast cancer cancer chemotherapy cancer survival cell proliferation cell strain MCF 7 cell structure colon cancer controlled study enzyme activity enzyme linked immunosorbent assay female flow cytometry genetic transfection HeLa cell human human cell in vitro study in vivo study lung metastasis mouse nonhuman priority journal protein expression protein purification tumor growth Animals Apoptosis Breast Neoplasms Cell Line, Tumor Colonic Neoplasms Colorectal Neoplasms Female Gene Silencing Humans Lung Neoplasms Mammary Neoplasms, Animal Mice Mice, Inbred BALB C Secretory Leukocyte Peptidase Inhibitor Transfection Uterine Cervical Neoplasms Murinae Mus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v228_n2_p469_Amiano http://hdl.handle.net/20.500.12110/paper_00219541_v228_n2_p469_Amiano
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00219541_v228_n2_p469_Amiano
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spelling	paper:paper_00219541_v228_n2_p469_Amiano2023-06-08T14:43:49Z Anti-tumor effect of SLPI on mammary but not colon tumor growth Amiano, Nicolás Oscar Reiteri, Romina Macarena Guerrieri, Diego Orqueda, Andrés Javier caspase 3 secretory leukocyte proteinase inhibitor small interfering RNA animal cell animal experiment animal model antineoplastic activity apoptosis article breast adenocarcinoma breast cancer cancer chemotherapy cancer survival cell proliferation cell strain MCF 7 cell structure colon cancer controlled study enzyme activity enzyme linked immunosorbent assay female flow cytometry genetic transfection HeLa cell human human cell in vitro study in vivo study lung metastasis mouse nonhuman priority journal protein expression protein purification tumor growth Animals Apoptosis Breast Neoplasms Cell Line, Tumor Colonic Neoplasms Colorectal Neoplasms Female Gene Silencing Humans Lung Neoplasms Mammary Neoplasms, Animal Mice Mice, Inbred BALB C Secretory Leukocyte Peptidase Inhibitor Transfection Uterine Cervical Neoplasms Murinae Mus Secretory leukocyte protease inhibitor (SLPI) is a serine protease inhibitor that was related to cancer development and metastasis dissemination on several types of tumors. However, it is not known the effect of SLPI on mammary and colon tumors. The aim of this study was to examine the effect of SLPI on mammary and colon tumor growth. The effect of SLPI was tested on in vitro cell apoptosis and in vivo tumor growth experiments. SLPI over-expressing human and murine mammary and colon tumor cells were generated by gene transfection. The administration of murine mammary tumor cells over-expressing high levels of SLPI did not develop tumors in mice. On the contrary, the administration of murine colon tumor cells over-expressing SLPI, developed faster tumors than control cells. Intratumoral, but not intraperitoneal administration of SLPI, delayed the growth of tumors and increased the survival of mammary but not colon tumor bearing mice. In vitro culture of mammary tumor cell lines treated with SLPI, and SLPI producer clones were more prone to apoptosis than control cells, mainly under serum deprivation culture conditions. Herein we demonstrated that SLPI induces the apoptosis of mammary tumor cells in vitro and decreases the mammary but not colon tumor growth in vivo. Therefore, SLPI may be a new potential therapeutic tool for certain tumors, such as mammary tumors. © 2012 Wiley Periodicals, Inc. Fil:Amiano, N.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Reiteri, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guerrieri, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Orqueda, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v228_n2_p469_Amiano http://hdl.handle.net/20.500.12110/paper_00219541_v228_n2_p469_Amiano
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	caspase 3 secretory leukocyte proteinase inhibitor small interfering RNA animal cell animal experiment animal model antineoplastic activity apoptosis article breast adenocarcinoma breast cancer cancer chemotherapy cancer survival cell proliferation cell strain MCF 7 cell structure colon cancer controlled study enzyme activity enzyme linked immunosorbent assay female flow cytometry genetic transfection HeLa cell human human cell in vitro study in vivo study lung metastasis mouse nonhuman priority journal protein expression protein purification tumor growth Animals Apoptosis Breast Neoplasms Cell Line, Tumor Colonic Neoplasms Colorectal Neoplasms Female Gene Silencing Humans Lung Neoplasms Mammary Neoplasms, Animal Mice Mice, Inbred BALB C Secretory Leukocyte Peptidase Inhibitor Transfection Uterine Cervical Neoplasms Murinae Mus
spellingShingle	caspase 3 secretory leukocyte proteinase inhibitor small interfering RNA animal cell animal experiment animal model antineoplastic activity apoptosis article breast adenocarcinoma breast cancer cancer chemotherapy cancer survival cell proliferation cell strain MCF 7 cell structure colon cancer controlled study enzyme activity enzyme linked immunosorbent assay female flow cytometry genetic transfection HeLa cell human human cell in vitro study in vivo study lung metastasis mouse nonhuman priority journal protein expression protein purification tumor growth Animals Apoptosis Breast Neoplasms Cell Line, Tumor Colonic Neoplasms Colorectal Neoplasms Female Gene Silencing Humans Lung Neoplasms Mammary Neoplasms, Animal Mice Mice, Inbred BALB C Secretory Leukocyte Peptidase Inhibitor Transfection Uterine Cervical Neoplasms Murinae Mus Amiano, Nicolás Oscar Reiteri, Romina Macarena Guerrieri, Diego Orqueda, Andrés Javier Anti-tumor effect of SLPI on mammary but not colon tumor growth
topic_facet	caspase 3 secretory leukocyte proteinase inhibitor small interfering RNA animal cell animal experiment animal model antineoplastic activity apoptosis article breast adenocarcinoma breast cancer cancer chemotherapy cancer survival cell proliferation cell strain MCF 7 cell structure colon cancer controlled study enzyme activity enzyme linked immunosorbent assay female flow cytometry genetic transfection HeLa cell human human cell in vitro study in vivo study lung metastasis mouse nonhuman priority journal protein expression protein purification tumor growth Animals Apoptosis Breast Neoplasms Cell Line, Tumor Colonic Neoplasms Colorectal Neoplasms Female Gene Silencing Humans Lung Neoplasms Mammary Neoplasms, Animal Mice Mice, Inbred BALB C Secretory Leukocyte Peptidase Inhibitor Transfection Uterine Cervical Neoplasms Murinae Mus
description	Secretory leukocyte protease inhibitor (SLPI) is a serine protease inhibitor that was related to cancer development and metastasis dissemination on several types of tumors. However, it is not known the effect of SLPI on mammary and colon tumors. The aim of this study was to examine the effect of SLPI on mammary and colon tumor growth. The effect of SLPI was tested on in vitro cell apoptosis and in vivo tumor growth experiments. SLPI over-expressing human and murine mammary and colon tumor cells were generated by gene transfection. The administration of murine mammary tumor cells over-expressing high levels of SLPI did not develop tumors in mice. On the contrary, the administration of murine colon tumor cells over-expressing SLPI, developed faster tumors than control cells. Intratumoral, but not intraperitoneal administration of SLPI, delayed the growth of tumors and increased the survival of mammary but not colon tumor bearing mice. In vitro culture of mammary tumor cell lines treated with SLPI, and SLPI producer clones were more prone to apoptosis than control cells, mainly under serum deprivation culture conditions. Herein we demonstrated that SLPI induces the apoptosis of mammary tumor cells in vitro and decreases the mammary but not colon tumor growth in vivo. Therefore, SLPI may be a new potential therapeutic tool for certain tumors, such as mammary tumors. © 2012 Wiley Periodicals, Inc.
author	Amiano, Nicolás Oscar Reiteri, Romina Macarena Guerrieri, Diego Orqueda, Andrés Javier
author_facet	Amiano, Nicolás Oscar Reiteri, Romina Macarena Guerrieri, Diego Orqueda, Andrés Javier
author_sort	Amiano, Nicolás Oscar
title	Anti-tumor effect of SLPI on mammary but not colon tumor growth
title_short	Anti-tumor effect of SLPI on mammary but not colon tumor growth
title_full	Anti-tumor effect of SLPI on mammary but not colon tumor growth
title_fullStr	Anti-tumor effect of SLPI on mammary but not colon tumor growth
title_full_unstemmed	Anti-tumor effect of SLPI on mammary but not colon tumor growth
title_sort	anti-tumor effect of slpi on mammary but not colon tumor growth
publishDate	2013
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v228_n2_p469_Amiano http://hdl.handle.net/20.500.12110/paper_00219541_v228_n2_p469_Amiano
work_keys_str_mv	AT amianonicolasoscar antitumoreffectofslpionmammarybutnotcolontumorgrowth AT reiterirominamacarena antitumoreffectofslpionmammarybutnotcolontumorgrowth AT guerrieridiego antitumoreffectofslpionmammarybutnotcolontumorgrowth AT orquedaandresjavier antitumoreffectofslpionmammarybutnotcolontumorgrowth
_version_	1768543594935746560

Anti-tumor effect of SLPI on mammary but not colon tumor growth

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