Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells

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During the preimplantation phase of pregnancy the endometrial stroma differentiates into decidua, a process that implies numerous morphological changes and is an example of physiological transdifferentiation. Here we show that UIII rat endometrial stromal cells cultured in the presence of calf serum...

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Autores principales:	Vallejo, Griselda, Maschi, Darío, Maronna, Ricardo Antonio, Saragüeta, Patricia
Publicado:	2010
Materias:	complementary DNA matrix protein procollagen C proteinase structural protein WT1 protein amino terminal sequence of split gene animal experiment article cell culture cell proliferation cell structure chromatin cold shock domain containing c2 gene cytoarchitecture decidualization DNA microarray eef1a1 gene endometrium cell female gene gene expression gene identification guanine nucleotide binding protein alpha 12 gene in vitro study multiple endocrine neoplasia 1 gene nonhuman pregnancy priority journal protein synthesis rat serum signal transduction tripartite motif containing 27 gene wilms tumor 1 gene wt1 gene Animals Cell Differentiation Decidua Down-Regulation Female Gene Expression Profiling Gene Expression Regulation, Developmental Oligonucleotide Array Sequence Analysis Pregnancy Rats Rats, Sprague-Dawley Reproducibility of Results Stromal Cells Up-Regulation Rattus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v222_n1_p127_Vallejo http://hdl.handle.net/20.500.12110/paper_00219541_v222_n1_p127_Vallejo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_00219541_v222_n1_p127_Vallejo2023-06-08T14:43:48Z Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells Vallejo, Griselda Maschi, Darío Maronna, Ricardo Antonio Saragüeta, Patricia complementary DNA matrix protein procollagen C proteinase structural protein WT1 protein amino terminal sequence of split gene animal experiment article cell culture cell proliferation cell structure chromatin cold shock domain containing c2 gene cytoarchitecture decidualization DNA microarray eef1a1 gene endometrium cell female gene gene expression gene identification guanine nucleotide binding protein alpha 12 gene in vitro study multiple endocrine neoplasia 1 gene nonhuman pregnancy priority journal protein synthesis rat serum signal transduction tripartite motif containing 27 gene wilms tumor 1 gene wt1 gene Animals Cell Differentiation Decidua Down-Regulation Female Gene Expression Profiling Gene Expression Regulation, Developmental Oligonucleotide Array Sequence Analysis Pregnancy Rats Rats, Sprague-Dawley Reproducibility of Results Stromal Cells Up-Regulation Rattus During the preimplantation phase of pregnancy the endometrial stroma differentiates into decidua, a process that implies numerous morphological changes and is an example of physiological transdifferentiation. Here we show that UIII rat endometrial stromal cells cultured in the presence of calf serum acquired morphological features of decidual cells and expressed decidual markers. To identify genes involved in decidualization we compared gene expression patterns of control and decidualized UIII cells using cDNA microarray. We found 322 annotated genes exhibiting significant differences in expression (>3-fold, fold discovery rate (FDR) >0.005), of which 312 have not been previously related to decidualization. Analysis of overrepresented functions revealed that protein synthesis, gene expression, and chromatin architecture and remodeling are the most relevant modified functions during decidualization. Relevant genes are also found in the functional terms differentiation, cell proliferation, signal transduction, and matrix/structural proteins. Several of these new genes involved in decidualization (Csdc2, Trim27, Eef1a1, Bmp1, Wt1, Aes, Gna12, and Men1) are shown to be also regulated in uterine decidua during normal pregnancy. Thus, the UIII cell culture model will allow future mechanistic studies to define the transcriptional network regulating reprogramming of stromal cells into decidual cells. © 2009 Wiley-Liss, Inc. Fil:Vallejo, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maschi, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maronna, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Saragüeta, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v222_n1_p127_Vallejo http://hdl.handle.net/20.500.12110/paper_00219541_v222_n1_p127_Vallejo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	complementary DNA matrix protein procollagen C proteinase structural protein WT1 protein amino terminal sequence of split gene animal experiment article cell culture cell proliferation cell structure chromatin cold shock domain containing c2 gene cytoarchitecture decidualization DNA microarray eef1a1 gene endometrium cell female gene gene expression gene identification guanine nucleotide binding protein alpha 12 gene in vitro study multiple endocrine neoplasia 1 gene nonhuman pregnancy priority journal protein synthesis rat serum signal transduction tripartite motif containing 27 gene wilms tumor 1 gene wt1 gene Animals Cell Differentiation Decidua Down-Regulation Female Gene Expression Profiling Gene Expression Regulation, Developmental Oligonucleotide Array Sequence Analysis Pregnancy Rats Rats, Sprague-Dawley Reproducibility of Results Stromal Cells Up-Regulation Rattus
spellingShingle	complementary DNA matrix protein procollagen C proteinase structural protein WT1 protein amino terminal sequence of split gene animal experiment article cell culture cell proliferation cell structure chromatin cold shock domain containing c2 gene cytoarchitecture decidualization DNA microarray eef1a1 gene endometrium cell female gene gene expression gene identification guanine nucleotide binding protein alpha 12 gene in vitro study multiple endocrine neoplasia 1 gene nonhuman pregnancy priority journal protein synthesis rat serum signal transduction tripartite motif containing 27 gene wilms tumor 1 gene wt1 gene Animals Cell Differentiation Decidua Down-Regulation Female Gene Expression Profiling Gene Expression Regulation, Developmental Oligonucleotide Array Sequence Analysis Pregnancy Rats Rats, Sprague-Dawley Reproducibility of Results Stromal Cells Up-Regulation Rattus Vallejo, Griselda Maschi, Darío Maronna, Ricardo Antonio Saragüeta, Patricia Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells
topic_facet	complementary DNA matrix protein procollagen C proteinase structural protein WT1 protein amino terminal sequence of split gene animal experiment article cell culture cell proliferation cell structure chromatin cold shock domain containing c2 gene cytoarchitecture decidualization DNA microarray eef1a1 gene endometrium cell female gene gene expression gene identification guanine nucleotide binding protein alpha 12 gene in vitro study multiple endocrine neoplasia 1 gene nonhuman pregnancy priority journal protein synthesis rat serum signal transduction tripartite motif containing 27 gene wilms tumor 1 gene wt1 gene Animals Cell Differentiation Decidua Down-Regulation Female Gene Expression Profiling Gene Expression Regulation, Developmental Oligonucleotide Array Sequence Analysis Pregnancy Rats Rats, Sprague-Dawley Reproducibility of Results Stromal Cells Up-Regulation Rattus
description	During the preimplantation phase of pregnancy the endometrial stroma differentiates into decidua, a process that implies numerous morphological changes and is an example of physiological transdifferentiation. Here we show that UIII rat endometrial stromal cells cultured in the presence of calf serum acquired morphological features of decidual cells and expressed decidual markers. To identify genes involved in decidualization we compared gene expression patterns of control and decidualized UIII cells using cDNA microarray. We found 322 annotated genes exhibiting significant differences in expression (>3-fold, fold discovery rate (FDR) >0.005), of which 312 have not been previously related to decidualization. Analysis of overrepresented functions revealed that protein synthesis, gene expression, and chromatin architecture and remodeling are the most relevant modified functions during decidualization. Relevant genes are also found in the functional terms differentiation, cell proliferation, signal transduction, and matrix/structural proteins. Several of these new genes involved in decidualization (Csdc2, Trim27, Eef1a1, Bmp1, Wt1, Aes, Gna12, and Men1) are shown to be also regulated in uterine decidua during normal pregnancy. Thus, the UIII cell culture model will allow future mechanistic studies to define the transcriptional network regulating reprogramming of stromal cells into decidual cells. © 2009 Wiley-Liss, Inc.
author	Vallejo, Griselda Maschi, Darío Maronna, Ricardo Antonio Saragüeta, Patricia
author_facet	Vallejo, Griselda Maschi, Darío Maronna, Ricardo Antonio Saragüeta, Patricia
author_sort	Vallejo, Griselda
title	Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells
title_short	Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells
title_full	Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells
title_fullStr	Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells
title_full_unstemmed	Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells
title_sort	changes in global gene expression during in vitro decidualization of rat endometrial stromal cells
publishDate	2010
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v222_n1_p127_Vallejo http://hdl.handle.net/20.500.12110/paper_00219541_v222_n1_p127_Vallejo
work_keys_str_mv	AT vallejogriselda changesinglobalgeneexpressionduringinvitrodecidualizationofratendometrialstromalcells AT maschidario changesinglobalgeneexpressionduringinvitrodecidualizationofratendometrialstromalcells AT maronnaricardoantonio changesinglobalgeneexpressionduringinvitrodecidualizationofratendometrialstromalcells AT saraguetapatricia changesinglobalgeneexpressionduringinvitrodecidualizationofratendometrialstromalcells
_version_	1768545358974025728

Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells

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