Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells

In order to study the effect of polyamine depletion on growth and proliferation of untransformed and chemically transformed cells, α‐difluoromethyl‐ornithine (DFMO) was added to cultures of 3T3 cells and their benzo[a]pyrene derivative BP‐3T3. Both types of cells stopped their proliferation after 72...

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Detalles Bibliográficos
Autores principales:	Goldemberg, Sara Hebe, Algranati, Israel David
Publicado:	1983
Materias:	benzo[a]pyrene eflornithine polyamine putrescine radioisotope spermidine animal cell cancer chemotherapy cell culture cell proliferation cell transformation drug therapy in vitro study mouse nonhuman reticuloendothelial system therapy thymidine h 3 Animal Benzo(a)pyrene Benzopyrenes Carcinogens Cell Division Cell Transformation, Neoplastic Cells, Cultured DNA Replication Eflornithine Interphase Kinetics Mice Mice, Inbred BALB C Ornithine Ornithine Decarboxylase Putrescine Support, Non-U.S. Gov't Transcription, Genetic Translation, Genetic
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v117_n2_p141_Medrano http://hdl.handle.net/20.500.12110/paper_00219541_v117_n2_p141_Medrano
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00219541_v117_n2_p141_Medrano
record_format	dspace
spelling	paper:paper_00219541_v117_n2_p141_Medrano2023-06-08T14:43:47Z Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells Goldemberg, Sara Hebe Algranati, Israel David benzo[a]pyrene eflornithine polyamine putrescine radioisotope spermidine animal cell cancer chemotherapy cell culture cell proliferation cell transformation drug therapy in vitro study mouse nonhuman reticuloendothelial system therapy thymidine h 3 Animal Benzo(a)pyrene Benzopyrenes Carcinogens Cell Division Cell Transformation, Neoplastic Cells, Cultured DNA Replication Eflornithine Interphase Kinetics Mice Mice, Inbred BALB C Ornithine Ornithine Decarboxylase Putrescine Support, Non-U.S. Gov't Transcription, Genetic Translation, Genetic In order to study the effect of polyamine depletion on growth and proliferation of untransformed and chemically transformed cells, α‐difluoromethyl‐ornithine (DFMO) was added to cultures of 3T3 cells and their benzo[a]pyrene derivative BP‐3T3. Both types of cells stopped their proliferation after 72 hr of treatment with the inhibitor. When DFMO was removed and cells were cultivated afterwards in fresh medium without the drug, untransformed cells resumed growth after a lag period, whereas transformed cells were unable to proliferate unless exogenous polyamine was added. These alterations showed a strict correlation with intracellular polyamine pools, since after removal of DFMO from the culture medium, polyamine concentrations increased to almost normal values in 3T3 cells, but remained at low levels or decreased even more in the transformed cells BP‐3T3. The analysis by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of labeled proteins of both cell extracts has indicated that the described control of cell proliferation by intracellular polyamine levels might be related to the synthesis of at least two proteins with molecular weights of about 36,000 and 55,000 daltons. Copyright © 1983 Wiley‐Liss, Inc. Fil:Goldemberg, S.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1983 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v117_n2_p141_Medrano http://hdl.handle.net/20.500.12110/paper_00219541_v117_n2_p141_Medrano
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	benzo[a]pyrene eflornithine polyamine putrescine radioisotope spermidine animal cell cancer chemotherapy cell culture cell proliferation cell transformation drug therapy in vitro study mouse nonhuman reticuloendothelial system therapy thymidine h 3 Animal Benzo(a)pyrene Benzopyrenes Carcinogens Cell Division Cell Transformation, Neoplastic Cells, Cultured DNA Replication Eflornithine Interphase Kinetics Mice Mice, Inbred BALB C Ornithine Ornithine Decarboxylase Putrescine Support, Non-U.S. Gov't Transcription, Genetic Translation, Genetic
spellingShingle	benzo[a]pyrene eflornithine polyamine putrescine radioisotope spermidine animal cell cancer chemotherapy cell culture cell proliferation cell transformation drug therapy in vitro study mouse nonhuman reticuloendothelial system therapy thymidine h 3 Animal Benzo(a)pyrene Benzopyrenes Carcinogens Cell Division Cell Transformation, Neoplastic Cells, Cultured DNA Replication Eflornithine Interphase Kinetics Mice Mice, Inbred BALB C Ornithine Ornithine Decarboxylase Putrescine Support, Non-U.S. Gov't Transcription, Genetic Translation, Genetic Goldemberg, Sara Hebe Algranati, Israel David Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells
topic_facet	benzo[a]pyrene eflornithine polyamine putrescine radioisotope spermidine animal cell cancer chemotherapy cell culture cell proliferation cell transformation drug therapy in vitro study mouse nonhuman reticuloendothelial system therapy thymidine h 3 Animal Benzo(a)pyrene Benzopyrenes Carcinogens Cell Division Cell Transformation, Neoplastic Cells, Cultured DNA Replication Eflornithine Interphase Kinetics Mice Mice, Inbred BALB C Ornithine Ornithine Decarboxylase Putrescine Support, Non-U.S. Gov't Transcription, Genetic Translation, Genetic
description	In order to study the effect of polyamine depletion on growth and proliferation of untransformed and chemically transformed cells, α‐difluoromethyl‐ornithine (DFMO) was added to cultures of 3T3 cells and their benzo[a]pyrene derivative BP‐3T3. Both types of cells stopped their proliferation after 72 hr of treatment with the inhibitor. When DFMO was removed and cells were cultivated afterwards in fresh medium without the drug, untransformed cells resumed growth after a lag period, whereas transformed cells were unable to proliferate unless exogenous polyamine was added. These alterations showed a strict correlation with intracellular polyamine pools, since after removal of DFMO from the culture medium, polyamine concentrations increased to almost normal values in 3T3 cells, but remained at low levels or decreased even more in the transformed cells BP‐3T3. The analysis by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of labeled proteins of both cell extracts has indicated that the described control of cell proliferation by intracellular polyamine levels might be related to the synthesis of at least two proteins with molecular weights of about 36,000 and 55,000 daltons. Copyright © 1983 Wiley‐Liss, Inc.
author	Goldemberg, Sara Hebe Algranati, Israel David
author_facet	Goldemberg, Sara Hebe Algranati, Israel David
author_sort	Goldemberg, Sara Hebe
title	Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells
title_short	Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells
title_full	Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells
title_fullStr	Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells
title_full_unstemmed	Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells
title_sort	differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3t3 and chemically transformed 3t3 cells
publishDate	1983
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v117_n2_p141_Medrano http://hdl.handle.net/20.500.12110/paper_00219541_v117_n2_p141_Medrano
work_keys_str_mv	AT goldembergsarahebe differentialeffectofadifluoromethylornithineontheproliferationofbalb3t3andchemicallytransformed3t3cells AT algranatiisraeldavid differentialeffectofadifluoromethylornithineontheproliferationofbalb3t3andchemicallytransformed3t3cells
_version_	1768543497506258944

Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells

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