Dynein and kinesin regulate stress-granule and P-body dynamics
Stress granules (SGs) and P-bodies (PBs) are related cytoplasmic structures harboring silenced mRNAs. SGs assemble transiently upon cellular stress, whereas PBs are constitutive and are further induced by stress. Both foci are highly dynamic, with messenger ribonucleoproteins (mRNPs) and proteins ra...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219533_v122_n21_p3973_Loschi http://hdl.handle.net/20.500.12110/paper_00219533_v122_n21_p3973_Loschi |
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paper:paper_00219533_v122_n21_p3973_Loschi2023-06-08T14:43:42Z Dynein and kinesin regulate stress-granule and P-body dynamics Loschi, Mariela Bicaudal Dynein Kinesin P-body Stress granule dynein adenosine triphosphatase kinesin animal cell article cell stress cell structure controlled study dissolution endoplasmic reticulum stress heavy chain light chain molecular dynamics nonhuman oxidative stress priority journal processing bodies protein transport regulatory mechanism stress granule Animals Cytoplasmic Structures Dyneins Kinesin Mice Microtubule-Associated Proteins NIH 3T3 Cells Protein Biosynthesis Mammalia Stress granules (SGs) and P-bodies (PBs) are related cytoplasmic structures harboring silenced mRNAs. SGs assemble transiently upon cellular stress, whereas PBs are constitutive and are further induced by stress. Both foci are highly dynamic, with messenger ribonucleoproteins (mRNPs) and proteins rapidly shuttling in and out. Here, we show that impairment of retrograde transport by knockdown of mammalian dynein heavy chain 1 (DHC1) or bicaudal D1 (BicD1) inhibits SG formation and PB growth upon stress, without affecting proteinsynthesis blockage. Conversely, impairment of anterograde transport by knockdown of kinesin-1 heavy chain (KIF5B) or kinesin light chain 1 (KLC1) delayed SG dissolution. Strikingly, SG dissolution is not required to restore translation. Simultaneous knockdown of dynein and kinesin reverted the effect of single knockdowns on both SGs and PBs, suggesting that a balance between opposing movements driven by these molecular motors governs foci formation and dissolution. Finally, we found that regulation of SG dynamics by dynein and kinesin is conserved in Drosophila. Fil:Loschi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219533_v122_n21_p3973_Loschi http://hdl.handle.net/20.500.12110/paper_00219533_v122_n21_p3973_Loschi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bicaudal Dynein Kinesin P-body Stress granule dynein adenosine triphosphatase kinesin animal cell article cell stress cell structure controlled study dissolution endoplasmic reticulum stress heavy chain light chain molecular dynamics nonhuman oxidative stress priority journal processing bodies protein transport regulatory mechanism stress granule Animals Cytoplasmic Structures Dyneins Kinesin Mice Microtubule-Associated Proteins NIH 3T3 Cells Protein Biosynthesis Mammalia |
| spellingShingle |
Bicaudal Dynein Kinesin P-body Stress granule dynein adenosine triphosphatase kinesin animal cell article cell stress cell structure controlled study dissolution endoplasmic reticulum stress heavy chain light chain molecular dynamics nonhuman oxidative stress priority journal processing bodies protein transport regulatory mechanism stress granule Animals Cytoplasmic Structures Dyneins Kinesin Mice Microtubule-Associated Proteins NIH 3T3 Cells Protein Biosynthesis Mammalia Loschi, Mariela Dynein and kinesin regulate stress-granule and P-body dynamics |
| topic_facet |
Bicaudal Dynein Kinesin P-body Stress granule dynein adenosine triphosphatase kinesin animal cell article cell stress cell structure controlled study dissolution endoplasmic reticulum stress heavy chain light chain molecular dynamics nonhuman oxidative stress priority journal processing bodies protein transport regulatory mechanism stress granule Animals Cytoplasmic Structures Dyneins Kinesin Mice Microtubule-Associated Proteins NIH 3T3 Cells Protein Biosynthesis Mammalia |
| description |
Stress granules (SGs) and P-bodies (PBs) are related cytoplasmic structures harboring silenced mRNAs. SGs assemble transiently upon cellular stress, whereas PBs are constitutive and are further induced by stress. Both foci are highly dynamic, with messenger ribonucleoproteins (mRNPs) and proteins rapidly shuttling in and out. Here, we show that impairment of retrograde transport by knockdown of mammalian dynein heavy chain 1 (DHC1) or bicaudal D1 (BicD1) inhibits SG formation and PB growth upon stress, without affecting proteinsynthesis blockage. Conversely, impairment of anterograde transport by knockdown of kinesin-1 heavy chain (KIF5B) or kinesin light chain 1 (KLC1) delayed SG dissolution. Strikingly, SG dissolution is not required to restore translation. Simultaneous knockdown of dynein and kinesin reverted the effect of single knockdowns on both SGs and PBs, suggesting that a balance between opposing movements driven by these molecular motors governs foci formation and dissolution. Finally, we found that regulation of SG dynamics by dynein and kinesin is conserved in Drosophila. |
| author |
Loschi, Mariela |
| author_facet |
Loschi, Mariela |
| author_sort |
Loschi, Mariela |
| title |
Dynein and kinesin regulate stress-granule and P-body dynamics |
| title_short |
Dynein and kinesin regulate stress-granule and P-body dynamics |
| title_full |
Dynein and kinesin regulate stress-granule and P-body dynamics |
| title_fullStr |
Dynein and kinesin regulate stress-granule and P-body dynamics |
| title_full_unstemmed |
Dynein and kinesin regulate stress-granule and P-body dynamics |
| title_sort |
dynein and kinesin regulate stress-granule and p-body dynamics |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219533_v122_n21_p3973_Loschi http://hdl.handle.net/20.500.12110/paper_00219533_v122_n21_p3973_Loschi |
| work_keys_str_mv |
AT loschimariela dyneinandkinesinregulatestressgranuleandpbodydynamics |
| _version_ |
1768546660846141440 |