In vivo and in vitro phosphorylation of two isoforms of yeast pyruvate kinase by protein kinase A
Saccharomyces cerevisiae pyruvate kinase 1 (Pyk1) was demonstrated to be associated to an immunoprecipitate of yeast protein kinase A holoenzyme (HA. Tpk1-Bcy1) and to be phosphorylated in a cAMP-dependent process. Both glutathione S-transferase (GST)-Pyk1 and GST-Pyk2 were phosphorylated in vitro b...
Guardado en:
| Autores principales: | , , |
|---|---|
| Publicado: |
2002
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v277_n34_p30477_Portela http://hdl.handle.net/20.500.12110/paper_00219258_v277_n34_p30477_Portela |
| Aporte de: |
| id |
paper:paper_00219258_v277_n34_p30477_Portela |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_00219258_v277_n34_p30477_Portela2023-06-08T14:43:21Z In vivo and in vitro phosphorylation of two isoforms of yeast pyruvate kinase by protein kinase A Portela, Paula Moreno de Colonna, Silvia Rossi, Silvia Graciela Catalysis Enzyme kinetics Enzymes Genes Yeast Phosphorylation Biochemistry cyclic AMP cyclic AMP dependent protein kinase fructose 1,6 bisphosphate glutathione transferase hybrid protein kemptide phosphoenolpyruvate pyruvate kinase article controlled study enzyme active site enzyme activity enzyme specificity enzyme substrate enzyme subunit immunoprecipitation in vitro study in vivo study matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman priority journal protein expression protein phosphorylation Saccharomyces cerevisiae strain difference titrimetry Animals Cattle Cyclic AMP-Dependent Protein Kinases Glycolysis Isoenzymes Kinetics Phosphorylation Pyruvate Kinase Saccharomyces cerevisiae Bovinae Saccharomyces Saccharomyces cerevisiae Saccharomyces cerevisiae pyruvate kinase 1 (Pyk1) was demonstrated to be associated to an immunoprecipitate of yeast protein kinase A holoenzyme (HA. Tpk1-Bcy1) and to be phosphorylated in a cAMP-dependent process. Both glutathione S-transferase (GST)-Pyk1 and GST-Pyk2 were phosphorylated in vitro by the bovine heart protein kinase A (PKA) catalytic subunit and by immobilized yeast HA-Tpk1. The specificity constant for the phosphorylation of GST-Pyk1 and GST-Pyk2 by bovine catalytic subunit was in the range of the value for Leu-Arg-Arg-Ala-Ser-Leu-Gly (Kemptide). Both fusion proteins were phosphorylated in vivo, in intact cells overexpressing the protein, or in vitro using crude extracts, as source of protein kinase A, when a wild type strain was used but were not phosphorylated when using a strain with only one TPK gene with an attenuated mutation (tpk1w1). The effect of phosphorylation on Pyk activity was assayed in partially purified preparations from three strains, containing different endogenous protein kinase A activity levels. Pyk1 activity was measured at different phosphoenolpyruvate concentrations in the absence or in the presence of the activator fructose 1,6-bisphosphate at 1.5 mM. Preliminary kinetic results derived from the comparison of Pyk1 obtained from extracts with the highest versus those from the lowest protein kinase A activity indicate that the enzyme is more active upon phosphorylation conditions; in the absence of the activator it shows a shift in the titration curve for phosphoenolpyruvate to the left and an increase in the Hill coefficient, whereas in the presence of fructose 1,6-bisphosphate it shows an nH value of 1.4, as compared with an nH of 2 for the Pyk1 obtained from extracts with almost null protein kinase A activity. Fil:Portela, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rossi, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v277_n34_p30477_Portela http://hdl.handle.net/20.500.12110/paper_00219258_v277_n34_p30477_Portela |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Catalysis Enzyme kinetics Enzymes Genes Yeast Phosphorylation Biochemistry cyclic AMP cyclic AMP dependent protein kinase fructose 1,6 bisphosphate glutathione transferase hybrid protein kemptide phosphoenolpyruvate pyruvate kinase article controlled study enzyme active site enzyme activity enzyme specificity enzyme substrate enzyme subunit immunoprecipitation in vitro study in vivo study matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman priority journal protein expression protein phosphorylation Saccharomyces cerevisiae strain difference titrimetry Animals Cattle Cyclic AMP-Dependent Protein Kinases Glycolysis Isoenzymes Kinetics Phosphorylation Pyruvate Kinase Saccharomyces cerevisiae Bovinae Saccharomyces Saccharomyces cerevisiae |
| spellingShingle |
Catalysis Enzyme kinetics Enzymes Genes Yeast Phosphorylation Biochemistry cyclic AMP cyclic AMP dependent protein kinase fructose 1,6 bisphosphate glutathione transferase hybrid protein kemptide phosphoenolpyruvate pyruvate kinase article controlled study enzyme active site enzyme activity enzyme specificity enzyme substrate enzyme subunit immunoprecipitation in vitro study in vivo study matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman priority journal protein expression protein phosphorylation Saccharomyces cerevisiae strain difference titrimetry Animals Cattle Cyclic AMP-Dependent Protein Kinases Glycolysis Isoenzymes Kinetics Phosphorylation Pyruvate Kinase Saccharomyces cerevisiae Bovinae Saccharomyces Saccharomyces cerevisiae Portela, Paula Moreno de Colonna, Silvia Rossi, Silvia Graciela In vivo and in vitro phosphorylation of two isoforms of yeast pyruvate kinase by protein kinase A |
| topic_facet |
Catalysis Enzyme kinetics Enzymes Genes Yeast Phosphorylation Biochemistry cyclic AMP cyclic AMP dependent protein kinase fructose 1,6 bisphosphate glutathione transferase hybrid protein kemptide phosphoenolpyruvate pyruvate kinase article controlled study enzyme active site enzyme activity enzyme specificity enzyme substrate enzyme subunit immunoprecipitation in vitro study in vivo study matrix assisted laser desorption ionization time of flight mass spectrometry nonhuman priority journal protein expression protein phosphorylation Saccharomyces cerevisiae strain difference titrimetry Animals Cattle Cyclic AMP-Dependent Protein Kinases Glycolysis Isoenzymes Kinetics Phosphorylation Pyruvate Kinase Saccharomyces cerevisiae Bovinae Saccharomyces Saccharomyces cerevisiae |
| description |
Saccharomyces cerevisiae pyruvate kinase 1 (Pyk1) was demonstrated to be associated to an immunoprecipitate of yeast protein kinase A holoenzyme (HA. Tpk1-Bcy1) and to be phosphorylated in a cAMP-dependent process. Both glutathione S-transferase (GST)-Pyk1 and GST-Pyk2 were phosphorylated in vitro by the bovine heart protein kinase A (PKA) catalytic subunit and by immobilized yeast HA-Tpk1. The specificity constant for the phosphorylation of GST-Pyk1 and GST-Pyk2 by bovine catalytic subunit was in the range of the value for Leu-Arg-Arg-Ala-Ser-Leu-Gly (Kemptide). Both fusion proteins were phosphorylated in vivo, in intact cells overexpressing the protein, or in vitro using crude extracts, as source of protein kinase A, when a wild type strain was used but were not phosphorylated when using a strain with only one TPK gene with an attenuated mutation (tpk1w1). The effect of phosphorylation on Pyk activity was assayed in partially purified preparations from three strains, containing different endogenous protein kinase A activity levels. Pyk1 activity was measured at different phosphoenolpyruvate concentrations in the absence or in the presence of the activator fructose 1,6-bisphosphate at 1.5 mM. Preliminary kinetic results derived from the comparison of Pyk1 obtained from extracts with the highest versus those from the lowest protein kinase A activity indicate that the enzyme is more active upon phosphorylation conditions; in the absence of the activator it shows a shift in the titration curve for phosphoenolpyruvate to the left and an increase in the Hill coefficient, whereas in the presence of fructose 1,6-bisphosphate it shows an nH value of 1.4, as compared with an nH of 2 for the Pyk1 obtained from extracts with almost null protein kinase A activity. |
| author |
Portela, Paula Moreno de Colonna, Silvia Rossi, Silvia Graciela |
| author_facet |
Portela, Paula Moreno de Colonna, Silvia Rossi, Silvia Graciela |
| author_sort |
Portela, Paula |
| title |
In vivo and in vitro phosphorylation of two isoforms of yeast pyruvate kinase by protein kinase A |
| title_short |
In vivo and in vitro phosphorylation of two isoforms of yeast pyruvate kinase by protein kinase A |
| title_full |
In vivo and in vitro phosphorylation of two isoforms of yeast pyruvate kinase by protein kinase A |
| title_fullStr |
In vivo and in vitro phosphorylation of two isoforms of yeast pyruvate kinase by protein kinase A |
| title_full_unstemmed |
In vivo and in vitro phosphorylation of two isoforms of yeast pyruvate kinase by protein kinase A |
| title_sort |
in vivo and in vitro phosphorylation of two isoforms of yeast pyruvate kinase by protein kinase a |
| publishDate |
2002 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v277_n34_p30477_Portela http://hdl.handle.net/20.500.12110/paper_00219258_v277_n34_p30477_Portela |
| work_keys_str_mv |
AT portelapaula invivoandinvitrophosphorylationoftwoisoformsofyeastpyruvatekinasebyproteinkinasea AT morenodecolonnasilvia invivoandinvitrophosphorylationoftwoisoformsofyeastpyruvatekinasebyproteinkinasea AT rossisilviagraciela invivoandinvitrophosphorylationoftwoisoformsofyeastpyruvatekinasebyproteinkinasea |
| _version_ |
1768542870814326784 |