Odorous secretions in anurans: Morphological and functional assessment of serous glands as a source of volatile compounds in the skin of the treefrog Hypsiboas pulchellus (Amphibia: Anura: Hylidae)
Serous (granular or venom) glands occur in the skin of almost all species of adult amphibians, and are thought to be the source of a great diversity of chemical compounds. Despite recent advances in their chemistry, odorous volatile substances are compounds that have received less attention, and unt...
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| Publicado: |
2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00218782_v228_n3_p430_Brunetti http://hdl.handle.net/20.500.12110/paper_00218782_v228_n3_p430_Brunetti |
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paper:paper_00218782_v228_n3_p430_Brunetti |
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dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Adrenergic response Chemical defence Granular glands Lipids Pharmacological stimuli Surface electric stimulation Volatile-producing glands adrenalin fatty acid mevalonic acid volatile agent fragrance adult animal experiment animal tissue Anura Article controlled study explant fatty acid metabolism histochemistry Hypsiboas pulchellus in vivo study mucus gland myoepithelium cell nonhuman odor priority journal serous gland skin gland skin secretion skin surface ultrastructure anatomy and histology animal Anura bodily secretions exocrine gland physiology secretion (process) skin transmission electron microscopy Animals Anura Bodily Secretions Exocrine Glands Microscopy, Electron, Transmission Odorants Skin |
| spellingShingle |
Adrenergic response Chemical defence Granular glands Lipids Pharmacological stimuli Surface electric stimulation Volatile-producing glands adrenalin fatty acid mevalonic acid volatile agent fragrance adult animal experiment animal tissue Anura Article controlled study explant fatty acid metabolism histochemistry Hypsiboas pulchellus in vivo study mucus gland myoepithelium cell nonhuman odor priority journal serous gland skin gland skin secretion skin surface ultrastructure anatomy and histology animal Anura bodily secretions exocrine gland physiology secretion (process) skin transmission electron microscopy Animals Anura Bodily Secretions Exocrine Glands Microscopy, Electron, Transmission Odorants Skin Hermida, Gladys N. Iurman, Mariana Gabriela Faivovich, Julián Odorous secretions in anurans: Morphological and functional assessment of serous glands as a source of volatile compounds in the skin of the treefrog Hypsiboas pulchellus (Amphibia: Anura: Hylidae) |
| topic_facet |
Adrenergic response Chemical defence Granular glands Lipids Pharmacological stimuli Surface electric stimulation Volatile-producing glands adrenalin fatty acid mevalonic acid volatile agent fragrance adult animal experiment animal tissue Anura Article controlled study explant fatty acid metabolism histochemistry Hypsiboas pulchellus in vivo study mucus gland myoepithelium cell nonhuman odor priority journal serous gland skin gland skin secretion skin surface ultrastructure anatomy and histology animal Anura bodily secretions exocrine gland physiology secretion (process) skin transmission electron microscopy Animals Anura Bodily Secretions Exocrine Glands Microscopy, Electron, Transmission Odorants Skin |
| description |
Serous (granular or venom) glands occur in the skin of almost all species of adult amphibians, and are thought to be the source of a great diversity of chemical compounds. Despite recent advances in their chemistry, odorous volatile substances are compounds that have received less attention, and until now no study has attempted to associate histological data with the presence of these molecules in amphibians, or in any other vertebrate. Given the recent identification of 40 different volatile compounds from the skin secretions of H. pulchellus (a treefrog species that releases a strong odour when handled), we examined the structure, ultrastructure, histochemistry, and distribution of skin glands of this species. Histological analysis from six body regions reveals the presence of two types of glands that differ in their distribution. Mucous glands are homogeneously distributed, whereas serous glands are more numerous in the scapular region. Ultrastructural results indicate that electron-translucent vesicles observed within granules of serous glands are similar to those found in volatile-producing glands from insects and also with lipid vesicles from different organisms. Association among lipids and volatiles is also evidenced from chemical results, which indicate that at least some of the volatile components in H. pulchellus probably originate within the metabolism of fatty acids or the mevalonate pathway. As odorous secretions are often considered to be secreted under stress situations, the release of glandular content was assessed after pharmacological treatments, epinephrine administrated in vivo and on skin explants, and through surface electrical stimulation. Serous glands responded to all treatments, generally through an obvious contraction of myoepithelial cells that surround their secretory portion. No response was observed in mucous glands. Considering these morpho-functional results, along with previous identification of volatiles from H. pulchellus and H. riojanus after electrical stimulation, we suggest that the electron-translucent inclusions found within the granules of serous glands likely are the store sites of volatile compounds and/or their precursors. Histochemical and glandular distribution analyses in five other species of frogs of the hylid tribe Cophomantini, revealed a high lipid content in all the species, whereas a heterogeneous distribution of serous glands is only observed in species of the H. pulchellus group. The distribution pattern of serous glands in members of this species group, and the odorous volatile secretions are probably related to defensive functions. © 2016 Anatomical Society. |
| author |
Hermida, Gladys N. Iurman, Mariana Gabriela Faivovich, Julián |
| author_facet |
Hermida, Gladys N. Iurman, Mariana Gabriela Faivovich, Julián |
| author_sort |
Hermida, Gladys N. |
| title |
Odorous secretions in anurans: Morphological and functional assessment of serous glands as a source of volatile compounds in the skin of the treefrog Hypsiboas pulchellus (Amphibia: Anura: Hylidae) |
| title_short |
Odorous secretions in anurans: Morphological and functional assessment of serous glands as a source of volatile compounds in the skin of the treefrog Hypsiboas pulchellus (Amphibia: Anura: Hylidae) |
| title_full |
Odorous secretions in anurans: Morphological and functional assessment of serous glands as a source of volatile compounds in the skin of the treefrog Hypsiboas pulchellus (Amphibia: Anura: Hylidae) |
| title_fullStr |
Odorous secretions in anurans: Morphological and functional assessment of serous glands as a source of volatile compounds in the skin of the treefrog Hypsiboas pulchellus (Amphibia: Anura: Hylidae) |
| title_full_unstemmed |
Odorous secretions in anurans: Morphological and functional assessment of serous glands as a source of volatile compounds in the skin of the treefrog Hypsiboas pulchellus (Amphibia: Anura: Hylidae) |
| title_sort |
odorous secretions in anurans: morphological and functional assessment of serous glands as a source of volatile compounds in the skin of the treefrog hypsiboas pulchellus (amphibia: anura: hylidae) |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00218782_v228_n3_p430_Brunetti http://hdl.handle.net/20.500.12110/paper_00218782_v228_n3_p430_Brunetti |
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AT hermidagladysn odoroussecretionsinanuransmorphologicalandfunctionalassessmentofserousglandsasasourceofvolatilecompoundsintheskinofthetreefroghypsiboaspulchellusamphibiaanurahylidae AT iurmanmarianagabriela odoroussecretionsinanuransmorphologicalandfunctionalassessmentofserousglandsasasourceofvolatilecompoundsintheskinofthetreefroghypsiboaspulchellusamphibiaanurahylidae AT faivovichjulian odoroussecretionsinanuransmorphologicalandfunctionalassessmentofserousglandsasasourceofvolatilecompoundsintheskinofthetreefroghypsiboaspulchellusamphibiaanurahylidae |
| _version_ |
1768541736111439872 |
| spelling |
paper:paper_00218782_v228_n3_p430_Brunetti2023-06-08T14:42:33Z Odorous secretions in anurans: Morphological and functional assessment of serous glands as a source of volatile compounds in the skin of the treefrog Hypsiboas pulchellus (Amphibia: Anura: Hylidae) Hermida, Gladys N. Iurman, Mariana Gabriela Faivovich, Julián Adrenergic response Chemical defence Granular glands Lipids Pharmacological stimuli Surface electric stimulation Volatile-producing glands adrenalin fatty acid mevalonic acid volatile agent fragrance adult animal experiment animal tissue Anura Article controlled study explant fatty acid metabolism histochemistry Hypsiboas pulchellus in vivo study mucus gland myoepithelium cell nonhuman odor priority journal serous gland skin gland skin secretion skin surface ultrastructure anatomy and histology animal Anura bodily secretions exocrine gland physiology secretion (process) skin transmission electron microscopy Animals Anura Bodily Secretions Exocrine Glands Microscopy, Electron, Transmission Odorants Skin Serous (granular or venom) glands occur in the skin of almost all species of adult amphibians, and are thought to be the source of a great diversity of chemical compounds. Despite recent advances in their chemistry, odorous volatile substances are compounds that have received less attention, and until now no study has attempted to associate histological data with the presence of these molecules in amphibians, or in any other vertebrate. Given the recent identification of 40 different volatile compounds from the skin secretions of H. pulchellus (a treefrog species that releases a strong odour when handled), we examined the structure, ultrastructure, histochemistry, and distribution of skin glands of this species. Histological analysis from six body regions reveals the presence of two types of glands that differ in their distribution. Mucous glands are homogeneously distributed, whereas serous glands are more numerous in the scapular region. Ultrastructural results indicate that electron-translucent vesicles observed within granules of serous glands are similar to those found in volatile-producing glands from insects and also with lipid vesicles from different organisms. Association among lipids and volatiles is also evidenced from chemical results, which indicate that at least some of the volatile components in H. pulchellus probably originate within the metabolism of fatty acids or the mevalonate pathway. As odorous secretions are often considered to be secreted under stress situations, the release of glandular content was assessed after pharmacological treatments, epinephrine administrated in vivo and on skin explants, and through surface electrical stimulation. Serous glands responded to all treatments, generally through an obvious contraction of myoepithelial cells that surround their secretory portion. No response was observed in mucous glands. Considering these morpho-functional results, along with previous identification of volatiles from H. pulchellus and H. riojanus after electrical stimulation, we suggest that the electron-translucent inclusions found within the granules of serous glands likely are the store sites of volatile compounds and/or their precursors. Histochemical and glandular distribution analyses in five other species of frogs of the hylid tribe Cophomantini, revealed a high lipid content in all the species, whereas a heterogeneous distribution of serous glands is only observed in species of the H. pulchellus group. The distribution pattern of serous glands in members of this species group, and the odorous volatile secretions are probably related to defensive functions. © 2016 Anatomical Society. Fil:Hermida, G.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iurman, M.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Faivovich, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00218782_v228_n3_p430_Brunetti http://hdl.handle.net/20.500.12110/paper_00218782_v228_n3_p430_Brunetti |