Varying patterns of expression of insulin-like growth factors I and II and their receptors in murine mammary adenocarcinomas of different metastasizing ability
We studied the expression of insulin-like growth factors I (IGF-I) and II (IGF-II) and their receptors (IGF-R) in 2 related murine mammary adenocarcinoma in vivo lines, M3 and MM3 with different metastasizing ability. We further investigated the effects of IGFs on the secretion of a key enzyme in th...
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1996
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207136_v65_n6_p812_Guerra http://hdl.handle.net/20.500.12110/paper_00207136_v65_n6_p812_Guerra |
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paper:paper_00207136_v65_n6_p812_Guerra2023-06-08T14:41:22Z Varying patterns of expression of insulin-like growth factors I and II and their receptors in murine mammary adenocarcinomas of different metastasizing ability somatomedin b somatomedin c somatomedin receptor urokinase animal experiment animal model article breast adenocarcinoma controlled study female gene expression metastasis potential mouse nonhuman priority journal Adenocarcinoma Animals Cell Division Female Insulin-Like Growth Factor I Insulin-Like Growth Factor II Lung Neoplasms Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Neoplasm Transplantation Receptor, IGF Type 1 Receptor, IGF Type 2 RNA, Messenger Tumor Cells, Cultured Urinary Plasminogen Activator We studied the expression of insulin-like growth factors I (IGF-I) and II (IGF-II) and their receptors (IGF-R) in 2 related murine mammary adenocarcinoma in vivo lines, M3 and MM3 with different metastasizing ability. We further investigated the effects of IGFs on the secretion of a key enzyme in the metastatic cascade, the urokinase-type plasminogen activator (uPA) in M3 and MM3 cells. M3 is a spontaneous mammary tumor originated in BALB/c mice, with a 40% incidence of lung metastases. MM3 variant, obtained by successive s.c. implants of M3 lung metastases into syngeneic mice, shows a 95% incidence of lung metastases. Similar levels of expression of IGF-I protein were found in M3 and MM3 tumors, whereas IGF-II expression was 4-fold higher in MM3. RNAse protection assays showed similar levels of IGF-I mRNA in M3 and MM3 tumors and revealed a 4-fold increase in IGF-II transcripts in MM3 tumors compared with M3. Authentic IGF-I and II messages were also found in primary cultures of M3 and MM3 cells. IGF-I mRNA levels were similar in both cultures and, as described for solid tumors a 5-fold increase in IGF-II message was detected in MM3 cells. The presence of type I and mannose-6-phosphate (Man-6P)/type II IGF-R was demonstrated in both M3 and MM3 tumors. A 2-fold increase of type I IGF-R was detected in MM3 tumors compared with M3. Man-6P/ type II IGF-R levels were 2-fold lower in MM3 tumors than in M3. As observed in tumor membranes, type I IGF-R concentrations were higher and Man-6P/type II IGF-R lower in cultures of MM3 epithelial cells compared with MM3 cells. In addition, we found that IGF-I enhanced secreted uPA activity in both M3 and MM3 cells while IGF-II only stimulated uPA secretion in MM3 cells. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207136_v65_n6_p812_Guerra http://hdl.handle.net/20.500.12110/paper_00207136_v65_n6_p812_Guerra |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
somatomedin b somatomedin c somatomedin receptor urokinase animal experiment animal model article breast adenocarcinoma controlled study female gene expression metastasis potential mouse nonhuman priority journal Adenocarcinoma Animals Cell Division Female Insulin-Like Growth Factor I Insulin-Like Growth Factor II Lung Neoplasms Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Neoplasm Transplantation Receptor, IGF Type 1 Receptor, IGF Type 2 RNA, Messenger Tumor Cells, Cultured Urinary Plasminogen Activator |
| spellingShingle |
somatomedin b somatomedin c somatomedin receptor urokinase animal experiment animal model article breast adenocarcinoma controlled study female gene expression metastasis potential mouse nonhuman priority journal Adenocarcinoma Animals Cell Division Female Insulin-Like Growth Factor I Insulin-Like Growth Factor II Lung Neoplasms Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Neoplasm Transplantation Receptor, IGF Type 1 Receptor, IGF Type 2 RNA, Messenger Tumor Cells, Cultured Urinary Plasminogen Activator Varying patterns of expression of insulin-like growth factors I and II and their receptors in murine mammary adenocarcinomas of different metastasizing ability |
| topic_facet |
somatomedin b somatomedin c somatomedin receptor urokinase animal experiment animal model article breast adenocarcinoma controlled study female gene expression metastasis potential mouse nonhuman priority journal Adenocarcinoma Animals Cell Division Female Insulin-Like Growth Factor I Insulin-Like Growth Factor II Lung Neoplasms Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Neoplasm Transplantation Receptor, IGF Type 1 Receptor, IGF Type 2 RNA, Messenger Tumor Cells, Cultured Urinary Plasminogen Activator |
| description |
We studied the expression of insulin-like growth factors I (IGF-I) and II (IGF-II) and their receptors (IGF-R) in 2 related murine mammary adenocarcinoma in vivo lines, M3 and MM3 with different metastasizing ability. We further investigated the effects of IGFs on the secretion of a key enzyme in the metastatic cascade, the urokinase-type plasminogen activator (uPA) in M3 and MM3 cells. M3 is a spontaneous mammary tumor originated in BALB/c mice, with a 40% incidence of lung metastases. MM3 variant, obtained by successive s.c. implants of M3 lung metastases into syngeneic mice, shows a 95% incidence of lung metastases. Similar levels of expression of IGF-I protein were found in M3 and MM3 tumors, whereas IGF-II expression was 4-fold higher in MM3. RNAse protection assays showed similar levels of IGF-I mRNA in M3 and MM3 tumors and revealed a 4-fold increase in IGF-II transcripts in MM3 tumors compared with M3. Authentic IGF-I and II messages were also found in primary cultures of M3 and MM3 cells. IGF-I mRNA levels were similar in both cultures and, as described for solid tumors a 5-fold increase in IGF-II message was detected in MM3 cells. The presence of type I and mannose-6-phosphate (Man-6P)/type II IGF-R was demonstrated in both M3 and MM3 tumors. A 2-fold increase of type I IGF-R was detected in MM3 tumors compared with M3. Man-6P/ type II IGF-R levels were 2-fold lower in MM3 tumors than in M3. As observed in tumor membranes, type I IGF-R concentrations were higher and Man-6P/type II IGF-R lower in cultures of MM3 epithelial cells compared with MM3 cells. In addition, we found that IGF-I enhanced secreted uPA activity in both M3 and MM3 cells while IGF-II only stimulated uPA secretion in MM3 cells. |
| title |
Varying patterns of expression of insulin-like growth factors I and II and their receptors in murine mammary adenocarcinomas of different metastasizing ability |
| title_short |
Varying patterns of expression of insulin-like growth factors I and II and their receptors in murine mammary adenocarcinomas of different metastasizing ability |
| title_full |
Varying patterns of expression of insulin-like growth factors I and II and their receptors in murine mammary adenocarcinomas of different metastasizing ability |
| title_fullStr |
Varying patterns of expression of insulin-like growth factors I and II and their receptors in murine mammary adenocarcinomas of different metastasizing ability |
| title_full_unstemmed |
Varying patterns of expression of insulin-like growth factors I and II and their receptors in murine mammary adenocarcinomas of different metastasizing ability |
| title_sort |
varying patterns of expression of insulin-like growth factors i and ii and their receptors in murine mammary adenocarcinomas of different metastasizing ability |
| publishDate |
1996 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207136_v65_n6_p812_Guerra http://hdl.handle.net/20.500.12110/paper_00207136_v65_n6_p812_Guerra |
| _version_ |
1768541591414243328 |