A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria

1. l. A simple method for measuring erythrocyte porphobilinogenase activity has been devised. 2. 2. Red blood cells are hemolysed by three freeze-thaws, the hemolysate is diluted 15 times and only 0.5 ml of this preparation is used for determining porphobilinogenase. in an incubation system also con...

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Detalles Bibliográficos
Autor principal: Batlle, Alcira María del Carmen
Publicado: 1978
Materias:
erythrocyte enzyme
acute intermittent porphyria
blood and hemopoietic system
diagnosis
methodology
porphobilinogenase
Aminolevulinic Acid
Ammonia-Lyases
Comparative Study
Erythrocytes
Hemolysis
Human
Hydrogen-Ion Concentration
Porphobilinogen
Porphyria
Spectrophotometry
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v9_n12_p871_DelCBatlle
http://hdl.handle.net/20.500.12110/paper_0020711X_v9_n12_p871_DelCBatlle
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_0020711X_v9_n12_p871_DelCBatlle
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spelling paper:paper_0020711X_v9_n12_p871_DelCBatlle2023-06-08T14:41:17Z A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria Batlle, Alcira María del Carmen erythrocyte enzyme acute intermittent porphyria blood and hemopoietic system diagnosis methodology porphobilinogenase Aminolevulinic Acid Ammonia-Lyases Comparative Study Erythrocytes Hemolysis Human Hydrogen-Ion Concentration Porphobilinogen Porphyria Spectrophotometry 1. l. A simple method for measuring erythrocyte porphobilinogenase activity has been devised. 2. 2. Red blood cells are hemolysed by three freeze-thaws, the hemolysate is diluted 15 times and only 0.5 ml of this preparation is used for determining porphobilinogenase. in an incubation system also containing porphobilinogen, NaCl. MgCl2. Tris-HCl buffer pH 8.2; which is incubated aerobically, in the dark. at 45°. for 1 hr with shaking. 3. 3. The enzymic activity is quenched by adding TCA. 4. 4. Porphyrinogens are oxidized, protein precipitated centrifuged off and uroporphyrins formed are determined spectrophotometrically in the supernatant. 5. 5. The enzyme is stable up to 1 month if stored at -20°. © 1978. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1978 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v9_n12_p871_DelCBatlle http://hdl.handle.net/20.500.12110/paper_0020711X_v9_n12_p871_DelCBatlle
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic erythrocyte enzyme
acute intermittent porphyria
blood and hemopoietic system
diagnosis
methodology
porphobilinogenase
Aminolevulinic Acid
Ammonia-Lyases
Comparative Study
Erythrocytes
Hemolysis
Human
Hydrogen-Ion Concentration
Porphobilinogen
Porphyria
Spectrophotometry
spellingShingle erythrocyte enzyme
acute intermittent porphyria
blood and hemopoietic system
diagnosis
methodology
porphobilinogenase
Aminolevulinic Acid
Ammonia-Lyases
Comparative Study
Erythrocytes
Hemolysis
Human
Hydrogen-Ion Concentration
Porphobilinogen
Porphyria
Spectrophotometry
Batlle, Alcira María del Carmen
A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria
topic_facet erythrocyte enzyme
acute intermittent porphyria
blood and hemopoietic system
diagnosis
methodology
porphobilinogenase
Aminolevulinic Acid
Ammonia-Lyases
Comparative Study
Erythrocytes
Hemolysis
Human
Hydrogen-Ion Concentration
Porphobilinogen
Porphyria
Spectrophotometry
description 1. l. A simple method for measuring erythrocyte porphobilinogenase activity has been devised. 2. 2. Red blood cells are hemolysed by three freeze-thaws, the hemolysate is diluted 15 times and only 0.5 ml of this preparation is used for determining porphobilinogenase. in an incubation system also containing porphobilinogen, NaCl. MgCl2. Tris-HCl buffer pH 8.2; which is incubated aerobically, in the dark. at 45°. for 1 hr with shaking. 3. 3. The enzymic activity is quenched by adding TCA. 4. 4. Porphyrinogens are oxidized, protein precipitated centrifuged off and uroporphyrins formed are determined spectrophotometrically in the supernatant. 5. 5. The enzyme is stable up to 1 month if stored at -20°. © 1978.
author Batlle, Alcira María del Carmen
author_facet Batlle, Alcira María del Carmen
author_sort Batlle, Alcira María del Carmen
title A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria
title_short A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria
title_full A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria
title_fullStr A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria
title_full_unstemmed A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria
title_sort simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria
publishDate 1978
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v9_n12_p871_DelCBatlle
http://hdl.handle.net/20.500.12110/paper_0020711X_v9_n12_p871_DelCBatlle
work_keys_str_mv AT batllealciramariadelcarmen asimplemethodformeasuringerythrocyteporphobilinogenaseanditsuseinthediagnosisofacuteintermittentporphyria
AT batllealciramariadelcarmen simplemethodformeasuringerythrocyteporphobilinogenaseanditsuseinthediagnosisofacuteintermittentporphyria
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