Polypyrrole intermediates in porphyrin biosynthesis. Studies with Euglena gracilis

1. 1. Evidence for the formation of pyrrolic intermediates resulting from the action of a whole enzyme system and deaminase preparations from Euglena gracilis on porphobilinogen, under normal anaerobic conditions is reported. 2. 2. It was found that the activity of Euglena gracilis pellet was not af...

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Autores principales:	Rossetti, María Victoria, Batlle, Alcira María del Carmen
Publicado:	1977
Materias:	porphyrin euglena gracilis in vitro study microorganism theoretical study
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v8_n4_p277_Rossetti http://hdl.handle.net/20.500.12110/paper_0020711X_v8_n4_p277_Rossetti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0020711X_v8_n4_p277_Rossetti
record_format	dspace
spelling	paper:paper_0020711X_v8_n4_p277_Rossetti2023-06-08T14:41:16Z Polypyrrole intermediates in porphyrin biosynthesis. Studies with Euglena gracilis Rossetti, María Victoria Batlle, Alcira María del Carmen porphyrin euglena gracilis in vitro study microorganism theoretical study 1. 1. Evidence for the formation of pyrrolic intermediates resulting from the action of a whole enzyme system and deaminase preparations from Euglena gracilis on porphobilinogen, under normal anaerobic conditions is reported. 2. 2. It was found that the activity of Euglena gracilis pellet was not affected by the presence of air; the yield of porphyrinogens being the same under anaerobiosis and aerobiosis. Both with pellet and deaminase preparations, two phases in porphyrinogen synthesis were observed, a slow and a rapid one, suggesting that some intermediates in the reaction were formed. 3. 3. The nature of these intermediates was investigated by incubating them with different enzyme preparations and PBG. Results obtained suggested that the polypyrroles formed from the whole system and deaminase were different. They did not behave as substrates but as intermediates m the reaction and significantly inhibited total porphyrinogen formation. It is proposed that these intermediates could be in high percentage larger polypyrrylmethanes. © 1977. Fil:Rossetti, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1977 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v8_n4_p277_Rossetti http://hdl.handle.net/20.500.12110/paper_0020711X_v8_n4_p277_Rossetti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	porphyrin euglena gracilis in vitro study microorganism theoretical study
spellingShingle	porphyrin euglena gracilis in vitro study microorganism theoretical study Rossetti, María Victoria Batlle, Alcira María del Carmen Polypyrrole intermediates in porphyrin biosynthesis. Studies with Euglena gracilis
topic_facet	porphyrin euglena gracilis in vitro study microorganism theoretical study
description	1. 1. Evidence for the formation of pyrrolic intermediates resulting from the action of a whole enzyme system and deaminase preparations from Euglena gracilis on porphobilinogen, under normal anaerobic conditions is reported. 2. 2. It was found that the activity of Euglena gracilis pellet was not affected by the presence of air; the yield of porphyrinogens being the same under anaerobiosis and aerobiosis. Both with pellet and deaminase preparations, two phases in porphyrinogen synthesis were observed, a slow and a rapid one, suggesting that some intermediates in the reaction were formed. 3. 3. The nature of these intermediates was investigated by incubating them with different enzyme preparations and PBG. Results obtained suggested that the polypyrroles formed from the whole system and deaminase were different. They did not behave as substrates but as intermediates m the reaction and significantly inhibited total porphyrinogen formation. It is proposed that these intermediates could be in high percentage larger polypyrrylmethanes. © 1977.
author	Rossetti, María Victoria Batlle, Alcira María del Carmen
author_facet	Rossetti, María Victoria Batlle, Alcira María del Carmen
author_sort	Rossetti, María Victoria
title	Polypyrrole intermediates in porphyrin biosynthesis. Studies with Euglena gracilis
title_short	Polypyrrole intermediates in porphyrin biosynthesis. Studies with Euglena gracilis
title_full	Polypyrrole intermediates in porphyrin biosynthesis. Studies with Euglena gracilis
title_fullStr	Polypyrrole intermediates in porphyrin biosynthesis. Studies with Euglena gracilis
title_full_unstemmed	Polypyrrole intermediates in porphyrin biosynthesis. Studies with Euglena gracilis
title_sort	polypyrrole intermediates in porphyrin biosynthesis. studies with euglena gracilis
publishDate	1977
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v8_n4_p277_Rossetti http://hdl.handle.net/20.500.12110/paper_0020711X_v8_n4_p277_Rossetti
work_keys_str_mv	AT rossettimariavictoria polypyrroleintermediatesinporphyrinbiosynthesisstudieswitheuglenagracilis AT batllealciramariadelcarmen polypyrroleintermediatesinporphyrinbiosynthesisstudieswitheuglenagracilis
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Polypyrrole intermediates in porphyrin biosynthesis. Studies with Euglena gracilis

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