Thiol groups of soybean callus succinyl CoA synthetase
1. 1. The reaction between 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) and the SH groups of soybean callus succinyl-CoA synthetase has been investigated. At pH 8-6 and 25° C, four SH groups are titrable with DTNB in the native enzyme. No additional thiol groups have been revealed after unfolding of...
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1974
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v5_n2_p129_DeXifra http://hdl.handle.net/20.500.12110/paper_0020711X_v5_n2_p129_DeXifra |
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paper:paper_0020711X_v5_n2_p129_DeXifra2023-06-08T14:41:15Z Thiol groups of soybean callus succinyl CoA synthetase Batlle, Alcira María del Carmen 5 5′-dithio-bis-(2-nitrobenzoic acid) enzyme inactivation kinetics molecular weight changes soybean callus tissue subunits Succinyl-CoA synthetase thiol groups 1. 1. The reaction between 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) and the SH groups of soybean callus succinyl-CoA synthetase has been investigated. At pH 8-6 and 25° C, four SH groups are titrable with DTNB in the native enzyme. No additional thiol groups have been revealed after unfolding of the protein with 8 M urea. 2. 2. The loss of enzymatic activity paralleled the decrease in the number of free SH groups. As with p-mercuribenzoate and other mercurials, reaction with DTNB also resulted in dissociation of the enzyme into subunits. © 1974. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1974 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v5_n2_p129_DeXifra http://hdl.handle.net/20.500.12110/paper_0020711X_v5_n2_p129_DeXifra |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
5 5′-dithio-bis-(2-nitrobenzoic acid) enzyme inactivation kinetics molecular weight changes soybean callus tissue subunits Succinyl-CoA synthetase thiol groups |
| spellingShingle |
5 5′-dithio-bis-(2-nitrobenzoic acid) enzyme inactivation kinetics molecular weight changes soybean callus tissue subunits Succinyl-CoA synthetase thiol groups Batlle, Alcira María del Carmen Thiol groups of soybean callus succinyl CoA synthetase |
| topic_facet |
5 5′-dithio-bis-(2-nitrobenzoic acid) enzyme inactivation kinetics molecular weight changes soybean callus tissue subunits Succinyl-CoA synthetase thiol groups |
| description |
1. 1. The reaction between 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) and the SH groups of soybean callus succinyl-CoA synthetase has been investigated. At pH 8-6 and 25° C, four SH groups are titrable with DTNB in the native enzyme. No additional thiol groups have been revealed after unfolding of the protein with 8 M urea. 2. 2. The loss of enzymatic activity paralleled the decrease in the number of free SH groups. As with p-mercuribenzoate and other mercurials, reaction with DTNB also resulted in dissociation of the enzyme into subunits. © 1974. |
| author |
Batlle, Alcira María del Carmen |
| author_facet |
Batlle, Alcira María del Carmen |
| author_sort |
Batlle, Alcira María del Carmen |
| title |
Thiol groups of soybean callus succinyl CoA synthetase |
| title_short |
Thiol groups of soybean callus succinyl CoA synthetase |
| title_full |
Thiol groups of soybean callus succinyl CoA synthetase |
| title_fullStr |
Thiol groups of soybean callus succinyl CoA synthetase |
| title_full_unstemmed |
Thiol groups of soybean callus succinyl CoA synthetase |
| title_sort |
thiol groups of soybean callus succinyl coa synthetase |
| publishDate |
1974 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v5_n2_p129_DeXifra http://hdl.handle.net/20.500.12110/paper_0020711X_v5_n2_p129_DeXifra |
| work_keys_str_mv |
AT batllealciramariadelcarmen thiolgroupsofsoybeancallussuccinylcoasynthetase |
| _version_ |
1768543545374801920 |