Kinetics of porphyrin accumulation in cultured epithelial cells exposed to ALA
1. 1. The kinetics of porphyrin accumulation in cultured mammalian epithelial cells (CNCM-I-221) during exposure to ALA was investigated. 2. 2. The total porphyrin synthesized is a function of ALA concentration and the incubation time. The cellular porphyrin content exhibited a saturation pattern, r...
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1993
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paper:paper_0020711X_v25_n10_p1407_Fukuda2023-06-08T14:41:13Z Kinetics of porphyrin accumulation in cultured epithelial cells exposed to ALA Fukuda, Haydeé Batlle, Alcira María del Carmen aminolevulinic acid porphyrin animal cell article cell culture epithelium cell nonhuman Aminolevulinic Acid Animals Cell Cycle Cell Line Epithelial Cells Epithelium Kinetics Porphyrins Animalia Mammalia 1. 1. The kinetics of porphyrin accumulation in cultured mammalian epithelial cells (CNCM-I-221) during exposure to ALA was investigated. 2. 2. The total porphyrin synthesized is a function of ALA concentration and the incubation time. The cellular porphyrin content exhibited a saturation pattern, reaching a plateau at about 0.04 fmol porphyrins/cell. A biphasic time-dependent increase in the total porphyrin synthesized was observed. 3. 3. After 3 hr of exposure to ALA the rate of synthesis increased to ahnost twice the initial rate, reaching between 0.02 and 0.05 fmol porphyrins/cell/hr depending on serum concentration in the medium. 4. 4. Two effects of FBS on ALA-stimulated porphyrin accumulation were observed. Greater total porphyrin synthesis was found when incubations were made in 10% FBS compared to those in 1% FBS. 5. 5. The higher serum concentration also caused a greater release into the medium of the porphyrins generated in the cells with a calculated half-life of 24 min in 10% serum-supplemented medium compared with 62 min in 1% serum. 6. 6. The results obtained from cell synchronization experiments suggest that there is little obvious cell cycle-dependent variation in the synthesis of porphyrins from ALA. 7. 7. The small differences in the intracellular porphyrin content that were observed may be attributed to a slight reduction in the rate of loss of porphyrins in G2/M cells. © 1993. Fil:Fukuda, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A.M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1993 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v25_n10_p1407_Fukuda http://hdl.handle.net/20.500.12110/paper_0020711X_v25_n10_p1407_Fukuda |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
aminolevulinic acid porphyrin animal cell article cell culture epithelium cell nonhuman Aminolevulinic Acid Animals Cell Cycle Cell Line Epithelial Cells Epithelium Kinetics Porphyrins Animalia Mammalia |
| spellingShingle |
aminolevulinic acid porphyrin animal cell article cell culture epithelium cell nonhuman Aminolevulinic Acid Animals Cell Cycle Cell Line Epithelial Cells Epithelium Kinetics Porphyrins Animalia Mammalia Fukuda, Haydeé Batlle, Alcira María del Carmen Kinetics of porphyrin accumulation in cultured epithelial cells exposed to ALA |
| topic_facet |
aminolevulinic acid porphyrin animal cell article cell culture epithelium cell nonhuman Aminolevulinic Acid Animals Cell Cycle Cell Line Epithelial Cells Epithelium Kinetics Porphyrins Animalia Mammalia |
| description |
1. 1. The kinetics of porphyrin accumulation in cultured mammalian epithelial cells (CNCM-I-221) during exposure to ALA was investigated. 2. 2. The total porphyrin synthesized is a function of ALA concentration and the incubation time. The cellular porphyrin content exhibited a saturation pattern, reaching a plateau at about 0.04 fmol porphyrins/cell. A biphasic time-dependent increase in the total porphyrin synthesized was observed. 3. 3. After 3 hr of exposure to ALA the rate of synthesis increased to ahnost twice the initial rate, reaching between 0.02 and 0.05 fmol porphyrins/cell/hr depending on serum concentration in the medium. 4. 4. Two effects of FBS on ALA-stimulated porphyrin accumulation were observed. Greater total porphyrin synthesis was found when incubations were made in 10% FBS compared to those in 1% FBS. 5. 5. The higher serum concentration also caused a greater release into the medium of the porphyrins generated in the cells with a calculated half-life of 24 min in 10% serum-supplemented medium compared with 62 min in 1% serum. 6. 6. The results obtained from cell synchronization experiments suggest that there is little obvious cell cycle-dependent variation in the synthesis of porphyrins from ALA. 7. 7. The small differences in the intracellular porphyrin content that were observed may be attributed to a slight reduction in the rate of loss of porphyrins in G2/M cells. © 1993. |
| author |
Fukuda, Haydeé Batlle, Alcira María del Carmen |
| author_facet |
Fukuda, Haydeé Batlle, Alcira María del Carmen |
| author_sort |
Fukuda, Haydeé |
| title |
Kinetics of porphyrin accumulation in cultured epithelial cells exposed to ALA |
| title_short |
Kinetics of porphyrin accumulation in cultured epithelial cells exposed to ALA |
| title_full |
Kinetics of porphyrin accumulation in cultured epithelial cells exposed to ALA |
| title_fullStr |
Kinetics of porphyrin accumulation in cultured epithelial cells exposed to ALA |
| title_full_unstemmed |
Kinetics of porphyrin accumulation in cultured epithelial cells exposed to ALA |
| title_sort |
kinetics of porphyrin accumulation in cultured epithelial cells exposed to ala |
| publishDate |
1993 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v25_n10_p1407_Fukuda http://hdl.handle.net/20.500.12110/paper_0020711X_v25_n10_p1407_Fukuda |
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AT fukudahaydee kineticsofporphyrinaccumulationinculturedepithelialcellsexposedtoala AT batllealciramariadelcarmen kineticsofporphyrinaccumulationinculturedepithelialcellsexposedtoala |
| _version_ |
1768544943820767232 |