cAMP analogs and selective inhibitors used to study low Km Mucor rouxii cAMP phosphodiesterase
1. 1. The sensitivity of partially purified low Km, phosphodiesterase (PDE) from Mucor rouxii to pharmacological agents and cAMP analogs was studied. The IC50 obtained were compared with those reported for PDEs from higher eukaryotes. 2. 2. The best inhibitors of the hydrolysis of 1 gmM cAMP were SQ...
| Autores principales: | , |
|---|---|
| Publicado: |
1990
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v22_n9_p1047_Tomes http://hdl.handle.net/20.500.12110/paper_0020711X_v22_n9_p1047_Tomes |
| Aporte de: |
| id |
paper:paper_0020711X_v22_n9_p1047_Tomes |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_0020711X_v22_n9_p1047_Tomes2023-06-08T14:41:12Z cAMP analogs and selective inhibitors used to study low Km Mucor rouxii cAMP phosphodiesterase Tomes, Claudia Nora Moreno, Silvia 1 ethyl 4 hydrazino 1h pyrazolo[3,4 b]pyridine 5 carboxylic acid ethyl ester 4 (3 butoxy 4 methoxybenzyl) 2 imidazolidinone 4,5 dihydro 6 [4 (1h imidazol 1 yl)phenyl] 5 methyl 3(2h) pyridazinone 6 [4 (3 methylureido)phenyl] 3(2h) pyridazinone amrinone cilostamide cilostazol cyclic amp dipyridamole etazolate imazodan isobutylmethylxanthine milrinone papaverine phosphodiesterase phosphodiesterase inhibitor rolipram sq 65442 theophylline unclassified drug article fungus mucor nonhuman 1-Methyl-3-isobutylxanthine 3',5'-Cyclic-Nucleotide Phosphodiesterase Centrifugation, Density Gradient Cyclic AMP Cyclic GMP Dipyridamole Hydrolysis Mucor Nicotinic Acids Papaverine Pyridazines Support, Non-U.S. Gov't Theophylline Amylomyces rouxii Fungi Mucor 1. 1. The sensitivity of partially purified low Km, phosphodiesterase (PDE) from Mucor rouxii to pharmacological agents and cAMP analogs was studied. The IC50 obtained were compared with those reported for PDEs from higher eukaryotes. 2. 2. The best inhibitors of the hydrolysis of 1 gmM cAMP were SQ 65.442 (IC50, c 10 μM), dipyridamol and CI 930. cGMP was not an inhibitor (IC50 > 1000 μm). 3. 3. The cAMP analogs were tested as inhibitors of the hydrolysis of 0. l μM cAMP. 8-Aminohexylamino cAMP was the best inhibitor with an IC50 of c 1 μM. 4. 4. A sedimentation profile of Mucor PDE was assayed in the presence of several pharmacological inhibitors and eAMP analogs. No isoforms with different sensitivity towards the inhibitors were detected. Forms with slightly different behaviour towards some cAMP analogs were observed. © 1990. Fil:Tomes, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1990 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v22_n9_p1047_Tomes http://hdl.handle.net/20.500.12110/paper_0020711X_v22_n9_p1047_Tomes |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
1 ethyl 4 hydrazino 1h pyrazolo[3,4 b]pyridine 5 carboxylic acid ethyl ester 4 (3 butoxy 4 methoxybenzyl) 2 imidazolidinone 4,5 dihydro 6 [4 (1h imidazol 1 yl)phenyl] 5 methyl 3(2h) pyridazinone 6 [4 (3 methylureido)phenyl] 3(2h) pyridazinone amrinone cilostamide cilostazol cyclic amp dipyridamole etazolate imazodan isobutylmethylxanthine milrinone papaverine phosphodiesterase phosphodiesterase inhibitor rolipram sq 65442 theophylline unclassified drug article fungus mucor nonhuman 1-Methyl-3-isobutylxanthine 3',5'-Cyclic-Nucleotide Phosphodiesterase Centrifugation, Density Gradient Cyclic AMP Cyclic GMP Dipyridamole Hydrolysis Mucor Nicotinic Acids Papaverine Pyridazines Support, Non-U.S. Gov't Theophylline Amylomyces rouxii Fungi Mucor |
| spellingShingle |
1 ethyl 4 hydrazino 1h pyrazolo[3,4 b]pyridine 5 carboxylic acid ethyl ester 4 (3 butoxy 4 methoxybenzyl) 2 imidazolidinone 4,5 dihydro 6 [4 (1h imidazol 1 yl)phenyl] 5 methyl 3(2h) pyridazinone 6 [4 (3 methylureido)phenyl] 3(2h) pyridazinone amrinone cilostamide cilostazol cyclic amp dipyridamole etazolate imazodan isobutylmethylxanthine milrinone papaverine phosphodiesterase phosphodiesterase inhibitor rolipram sq 65442 theophylline unclassified drug article fungus mucor nonhuman 1-Methyl-3-isobutylxanthine 3',5'-Cyclic-Nucleotide Phosphodiesterase Centrifugation, Density Gradient Cyclic AMP Cyclic GMP Dipyridamole Hydrolysis Mucor Nicotinic Acids Papaverine Pyridazines Support, Non-U.S. Gov't Theophylline Amylomyces rouxii Fungi Mucor Tomes, Claudia Nora Moreno, Silvia cAMP analogs and selective inhibitors used to study low Km Mucor rouxii cAMP phosphodiesterase |
| topic_facet |
1 ethyl 4 hydrazino 1h pyrazolo[3,4 b]pyridine 5 carboxylic acid ethyl ester 4 (3 butoxy 4 methoxybenzyl) 2 imidazolidinone 4,5 dihydro 6 [4 (1h imidazol 1 yl)phenyl] 5 methyl 3(2h) pyridazinone 6 [4 (3 methylureido)phenyl] 3(2h) pyridazinone amrinone cilostamide cilostazol cyclic amp dipyridamole etazolate imazodan isobutylmethylxanthine milrinone papaverine phosphodiesterase phosphodiesterase inhibitor rolipram sq 65442 theophylline unclassified drug article fungus mucor nonhuman 1-Methyl-3-isobutylxanthine 3',5'-Cyclic-Nucleotide Phosphodiesterase Centrifugation, Density Gradient Cyclic AMP Cyclic GMP Dipyridamole Hydrolysis Mucor Nicotinic Acids Papaverine Pyridazines Support, Non-U.S. Gov't Theophylline Amylomyces rouxii Fungi Mucor |
| description |
1. 1. The sensitivity of partially purified low Km, phosphodiesterase (PDE) from Mucor rouxii to pharmacological agents and cAMP analogs was studied. The IC50 obtained were compared with those reported for PDEs from higher eukaryotes. 2. 2. The best inhibitors of the hydrolysis of 1 gmM cAMP were SQ 65.442 (IC50, c 10 μM), dipyridamol and CI 930. cGMP was not an inhibitor (IC50 > 1000 μm). 3. 3. The cAMP analogs were tested as inhibitors of the hydrolysis of 0. l μM cAMP. 8-Aminohexylamino cAMP was the best inhibitor with an IC50 of c 1 μM. 4. 4. A sedimentation profile of Mucor PDE was assayed in the presence of several pharmacological inhibitors and eAMP analogs. No isoforms with different sensitivity towards the inhibitors were detected. Forms with slightly different behaviour towards some cAMP analogs were observed. © 1990. |
| author |
Tomes, Claudia Nora Moreno, Silvia |
| author_facet |
Tomes, Claudia Nora Moreno, Silvia |
| author_sort |
Tomes, Claudia Nora |
| title |
cAMP analogs and selective inhibitors used to study low Km Mucor rouxii cAMP phosphodiesterase |
| title_short |
cAMP analogs and selective inhibitors used to study low Km Mucor rouxii cAMP phosphodiesterase |
| title_full |
cAMP analogs and selective inhibitors used to study low Km Mucor rouxii cAMP phosphodiesterase |
| title_fullStr |
cAMP analogs and selective inhibitors used to study low Km Mucor rouxii cAMP phosphodiesterase |
| title_full_unstemmed |
cAMP analogs and selective inhibitors used to study low Km Mucor rouxii cAMP phosphodiesterase |
| title_sort |
camp analogs and selective inhibitors used to study low km mucor rouxii camp phosphodiesterase |
| publishDate |
1990 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v22_n9_p1047_Tomes http://hdl.handle.net/20.500.12110/paper_0020711X_v22_n9_p1047_Tomes |
| work_keys_str_mv |
AT tomesclaudianora campanalogsandselectiveinhibitorsusedtostudylowkmmucorrouxiicampphosphodiesterase AT morenosilvia campanalogsandselectiveinhibitorsusedtostudylowkmmucorrouxiicampphosphodiesterase |
| _version_ |
1768541876500037632 |