Increased Expression of Aquaporin 9 in Trophoblast from Gestational Diabetic Patients
Gestational diabetes mellitus is the most frequent pathophysiological alteration in pregnancy, increasing the incidence of complications in both mother and fetus. The macrosomia that occurs in these fetuses may be related with some changes in nutrient transport mechanism in placenta. The presence of...
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paper:paper_00185043_v48_n8_p535_VilarinoGarcia2023-06-08T14:39:34Z Increased Expression of Aquaporin 9 in Trophoblast from Gestational Diabetic Patients Varone, Cecilia Laura AQP9 aquaporins gestational diabetes mellitus placenta aquaporin 9 glycerol AQP9 protein, human aquaporin messenger RNA adult Article cesarean section clinical article controlled study diabetic patient female gene expression human human tissue immunoblotting immunohistochemistry placenta pregnancy diabetes mellitus priority journal protein expression quantitative analysis real time polymerase chain reaction syncytiotrophoblast upregulation water transport gene expression regulation genetics metabolism pathology pregnancy pregnancy diabetes mellitus protein transport trophoblast Adult Aquaporins Diabetes, Gestational Female Gene Expression Regulation Humans Pregnancy Protein Transport RNA, Messenger Trophoblasts Gestational diabetes mellitus is the most frequent pathophysiological alteration in pregnancy, increasing the incidence of complications in both mother and fetus. The macrosomia that occurs in these fetuses may be related with some changes in nutrient transport mechanism in placenta. The presence of aquaporin 9, an aquaglyceroporin, has previously been demonstrated in placenta. We raised the question whether aquaporin 9 expression may be upregulated in placenta from gestational diabetes, thus providing a faster transport of glycerol and water through placenta. We studied 21 placentas (13 controls and 8 gestational diabetes) from cesarean delivery at term. The expression of aquaporin 9 was analyzed by quantitative PCR, immunoblot, and immunohistochemistry. The median values from quantitative PCR were compared by nonparametric tests for independent samples (Mann-Whitney U-test). We have found that trophoblast from gestational diabetes express higher amount of aquaporin 9, which was found statistically significant (p<0.05). The increase in aquaporin 9 expression was confirmed by immunoblot, and localization in the syncytiotrophoblast was checked by immunohistochemistry. The increase in aquaporin 9 expression in placenta from gestational diabetes may contribute to the higher transport rate in this pathology of pregnancy. © Georg Thieme Verlag KG Stuttgart, New York. Fil:Varone, C.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00185043_v48_n8_p535_VilarinoGarcia http://hdl.handle.net/20.500.12110/paper_00185043_v48_n8_p535_VilarinoGarcia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
AQP9 aquaporins gestational diabetes mellitus placenta aquaporin 9 glycerol AQP9 protein, human aquaporin messenger RNA adult Article cesarean section clinical article controlled study diabetic patient female gene expression human human tissue immunoblotting immunohistochemistry placenta pregnancy diabetes mellitus priority journal protein expression quantitative analysis real time polymerase chain reaction syncytiotrophoblast upregulation water transport gene expression regulation genetics metabolism pathology pregnancy pregnancy diabetes mellitus protein transport trophoblast Adult Aquaporins Diabetes, Gestational Female Gene Expression Regulation Humans Pregnancy Protein Transport RNA, Messenger Trophoblasts |
| spellingShingle |
AQP9 aquaporins gestational diabetes mellitus placenta aquaporin 9 glycerol AQP9 protein, human aquaporin messenger RNA adult Article cesarean section clinical article controlled study diabetic patient female gene expression human human tissue immunoblotting immunohistochemistry placenta pregnancy diabetes mellitus priority journal protein expression quantitative analysis real time polymerase chain reaction syncytiotrophoblast upregulation water transport gene expression regulation genetics metabolism pathology pregnancy pregnancy diabetes mellitus protein transport trophoblast Adult Aquaporins Diabetes, Gestational Female Gene Expression Regulation Humans Pregnancy Protein Transport RNA, Messenger Trophoblasts Varone, Cecilia Laura Increased Expression of Aquaporin 9 in Trophoblast from Gestational Diabetic Patients |
| topic_facet |
AQP9 aquaporins gestational diabetes mellitus placenta aquaporin 9 glycerol AQP9 protein, human aquaporin messenger RNA adult Article cesarean section clinical article controlled study diabetic patient female gene expression human human tissue immunoblotting immunohistochemistry placenta pregnancy diabetes mellitus priority journal protein expression quantitative analysis real time polymerase chain reaction syncytiotrophoblast upregulation water transport gene expression regulation genetics metabolism pathology pregnancy pregnancy diabetes mellitus protein transport trophoblast Adult Aquaporins Diabetes, Gestational Female Gene Expression Regulation Humans Pregnancy Protein Transport RNA, Messenger Trophoblasts |
| description |
Gestational diabetes mellitus is the most frequent pathophysiological alteration in pregnancy, increasing the incidence of complications in both mother and fetus. The macrosomia that occurs in these fetuses may be related with some changes in nutrient transport mechanism in placenta. The presence of aquaporin 9, an aquaglyceroporin, has previously been demonstrated in placenta. We raised the question whether aquaporin 9 expression may be upregulated in placenta from gestational diabetes, thus providing a faster transport of glycerol and water through placenta. We studied 21 placentas (13 controls and 8 gestational diabetes) from cesarean delivery at term. The expression of aquaporin 9 was analyzed by quantitative PCR, immunoblot, and immunohistochemistry. The median values from quantitative PCR were compared by nonparametric tests for independent samples (Mann-Whitney U-test). We have found that trophoblast from gestational diabetes express higher amount of aquaporin 9, which was found statistically significant (p<0.05). The increase in aquaporin 9 expression was confirmed by immunoblot, and localization in the syncytiotrophoblast was checked by immunohistochemistry. The increase in aquaporin 9 expression in placenta from gestational diabetes may contribute to the higher transport rate in this pathology of pregnancy. © Georg Thieme Verlag KG Stuttgart, New York. |
| author |
Varone, Cecilia Laura |
| author_facet |
Varone, Cecilia Laura |
| author_sort |
Varone, Cecilia Laura |
| title |
Increased Expression of Aquaporin 9 in Trophoblast from Gestational Diabetic Patients |
| title_short |
Increased Expression of Aquaporin 9 in Trophoblast from Gestational Diabetic Patients |
| title_full |
Increased Expression of Aquaporin 9 in Trophoblast from Gestational Diabetic Patients |
| title_fullStr |
Increased Expression of Aquaporin 9 in Trophoblast from Gestational Diabetic Patients |
| title_full_unstemmed |
Increased Expression of Aquaporin 9 in Trophoblast from Gestational Diabetic Patients |
| title_sort |
increased expression of aquaporin 9 in trophoblast from gestational diabetic patients |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00185043_v48_n8_p535_VilarinoGarcia http://hdl.handle.net/20.500.12110/paper_00185043_v48_n8_p535_VilarinoGarcia |
| work_keys_str_mv |
AT varonececilialaura increasedexpressionofaquaporin9introphoblastfromgestationaldiabeticpatients |
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