Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects

Insulin and leptin receptors are known to share signaling pathways, such as JAK2/STAT-3 (Janus kinase2/signal transduction and activator of transcription3), MAPK (Mitogen activated protein kinase), and PI3K (phosphoinositide 3-kinase). Both positive and negative cross-talk have been previously found...

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Autores principales:	Maymó, Julieta L., Varone, Cecilia Laura
Publicado:	2015
Materias:	gestational diabetes insulin leptin placenta signal transduction insulin receptor insulin receptor substrate IRS1 protein, human leptin receptor adult biological model case control study dose response down regulation female human immunohistochemistry metabolism phosphorylation pregnancy pregnancy diabetes mellitus Adult Case-Control Studies Diabetes, Gestational Dose-Response Relationship, Drug Down-Regulation Female Humans Immunohistochemistry Insulin Insulin Receptor Substrate Proteins Leptin Models, Biological Phosphorylation Placenta Pregnancy Receptor, Insulin Receptors, Leptin Signal Transduction
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00185043_v48_n1_p62_PerezPerez http://hdl.handle.net/20.500.12110/paper_00185043_v48_n1_p62_PerezPerez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00185043_v48_n1_p62_PerezPerez
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spelling	paper:paper_00185043_v48_n1_p62_PerezPerez2023-06-08T14:39:34Z Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects Maymó, Julieta L. Varone, Cecilia Laura gestational diabetes insulin leptin placenta signal transduction insulin insulin receptor insulin receptor substrate IRS1 protein, human leptin leptin receptor adult biological model case control study dose response down regulation female human immunohistochemistry metabolism phosphorylation placenta pregnancy pregnancy diabetes mellitus signal transduction Adult Case-Control Studies Diabetes, Gestational Dose-Response Relationship, Drug Down-Regulation Female Humans Immunohistochemistry Insulin Insulin Receptor Substrate Proteins Leptin Models, Biological Phosphorylation Placenta Pregnancy Receptor, Insulin Receptors, Leptin Signal Transduction Insulin and leptin receptors are known to share signaling pathways, such as JAK2/STAT-3 (Janus kinase2/signal transduction and activator of transcription3), MAPK (Mitogen activated protein kinase), and PI3K (phosphoinositide 3-kinase). Both positive and negative cross-talk have been previously found in different cellular systems. Gestational diabetes (GDM) is a pathophysiological state with high circulating levels of both insulin and leptin. We have previously found that these 3 signaling pathways are activated in placenta from GDM patients to promote translation, involving the activation of leptin receptor. Now, we have tested the hypothesis that both leptin and insulin receptors might contribute to this activation in a positive way that may become negative when the system is overactivated. We studied the activation of leptin and insulin receptors in placenta from GDM and healthy pregnancies. We have also performed in vitro studies with insulin and leptin stimulation of trophoblast explants from healthy placenta. We have found that both leptin and insulin receptors are activated in placenta from GDM. In vitro stimulation of trophoblast explants with both leptin and insulin at submaximal doses (0.1 nM) potentiated the activation of signaling, whereas preincubation with maximal concentrations of insulin (10 nM) and further stimulation with leptin showed negative effect. Trophoblastic explants from GDM placenta, which presented high signaling levels, had a negative signaling effect when further incubated in vitro with leptin. In conclusion, insulin and leptin receptors have positive effects on signaling, contributing to high signaling levels in GDM placenta, but insulin and leptin have negative effects upon overstimulation. © Georg Thieme Verlag KG Stuttgart · New York. Fil:Maymó, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Varone, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00185043_v48_n1_p62_PerezPerez http://hdl.handle.net/20.500.12110/paper_00185043_v48_n1_p62_PerezPerez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	gestational diabetes insulin leptin placenta signal transduction insulin insulin receptor insulin receptor substrate IRS1 protein, human leptin leptin receptor adult biological model case control study dose response down regulation female human immunohistochemistry metabolism phosphorylation placenta pregnancy pregnancy diabetes mellitus signal transduction Adult Case-Control Studies Diabetes, Gestational Dose-Response Relationship, Drug Down-Regulation Female Humans Immunohistochemistry Insulin Insulin Receptor Substrate Proteins Leptin Models, Biological Phosphorylation Placenta Pregnancy Receptor, Insulin Receptors, Leptin Signal Transduction
spellingShingle	gestational diabetes insulin leptin placenta signal transduction insulin insulin receptor insulin receptor substrate IRS1 protein, human leptin leptin receptor adult biological model case control study dose response down regulation female human immunohistochemistry metabolism phosphorylation placenta pregnancy pregnancy diabetes mellitus signal transduction Adult Case-Control Studies Diabetes, Gestational Dose-Response Relationship, Drug Down-Regulation Female Humans Immunohistochemistry Insulin Insulin Receptor Substrate Proteins Leptin Models, Biological Phosphorylation Placenta Pregnancy Receptor, Insulin Receptors, Leptin Signal Transduction Maymó, Julieta L. Varone, Cecilia Laura Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects
topic_facet	gestational diabetes insulin leptin placenta signal transduction insulin insulin receptor insulin receptor substrate IRS1 protein, human leptin leptin receptor adult biological model case control study dose response down regulation female human immunohistochemistry metabolism phosphorylation placenta pregnancy pregnancy diabetes mellitus signal transduction Adult Case-Control Studies Diabetes, Gestational Dose-Response Relationship, Drug Down-Regulation Female Humans Immunohistochemistry Insulin Insulin Receptor Substrate Proteins Leptin Models, Biological Phosphorylation Placenta Pregnancy Receptor, Insulin Receptors, Leptin Signal Transduction
description	Insulin and leptin receptors are known to share signaling pathways, such as JAK2/STAT-3 (Janus kinase2/signal transduction and activator of transcription3), MAPK (Mitogen activated protein kinase), and PI3K (phosphoinositide 3-kinase). Both positive and negative cross-talk have been previously found in different cellular systems. Gestational diabetes (GDM) is a pathophysiological state with high circulating levels of both insulin and leptin. We have previously found that these 3 signaling pathways are activated in placenta from GDM patients to promote translation, involving the activation of leptin receptor. Now, we have tested the hypothesis that both leptin and insulin receptors might contribute to this activation in a positive way that may become negative when the system is overactivated. We studied the activation of leptin and insulin receptors in placenta from GDM and healthy pregnancies. We have also performed in vitro studies with insulin and leptin stimulation of trophoblast explants from healthy placenta. We have found that both leptin and insulin receptors are activated in placenta from GDM. In vitro stimulation of trophoblast explants with both leptin and insulin at submaximal doses (0.1 nM) potentiated the activation of signaling, whereas preincubation with maximal concentrations of insulin (10 nM) and further stimulation with leptin showed negative effect. Trophoblastic explants from GDM placenta, which presented high signaling levels, had a negative signaling effect when further incubated in vitro with leptin. In conclusion, insulin and leptin receptors have positive effects on signaling, contributing to high signaling levels in GDM placenta, but insulin and leptin have negative effects upon overstimulation. © Georg Thieme Verlag KG Stuttgart · New York.
author	Maymó, Julieta L. Varone, Cecilia Laura
author_facet	Maymó, Julieta L. Varone, Cecilia Laura
author_sort	Maymó, Julieta L.
title	Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects
title_short	Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects
title_full	Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects
title_fullStr	Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects
title_full_unstemmed	Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects
title_sort	insulin and leptin signaling in placenta from gestational diabetic subjects
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00185043_v48_n1_p62_PerezPerez http://hdl.handle.net/20.500.12110/paper_00185043_v48_n1_p62_PerezPerez
work_keys_str_mv	AT maymojulietal insulinandleptinsignalinginplacentafromgestationaldiabeticsubjects AT varonececilialaura insulinandleptinsignalinginplacentafromgestationaldiabeticsubjects
_version_	1768542017773633536

Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects

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