Characterization of the gonadotropin-releasing hormone system in the Neotropical teleost, Steindachneridion parahybae during the annual reproductive cycle in captivity

This study evaluated by immunohistochemical and Western blot methods, the distribution of two distinct gonadotropin-releasing hormones (GnRHs), corresponding to catfish GnRH (cfGnRH or GnRH1) and chicken-II GnRH (cGnRH-II or GnRH2), in Steindachneridion parahybae females in captivity, focusing these...

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Detalles Bibliográficos
Publicado: 2019
Materias:
GnRH
Hypothalamus
Pituitary gland
Reproduction dysfunction
Seasonal reproduction
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v273_n_p73_Honji
http://hdl.handle.net/20.500.12110/paper_00166480_v273_n_p73_Honji
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00166480_v273_n_p73_Honji
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spelling paper:paper_00166480_v273_n_p73_Honji2023-06-08T14:38:23Z Characterization of the gonadotropin-releasing hormone system in the Neotropical teleost, Steindachneridion parahybae during the annual reproductive cycle in captivity GnRH Hypothalamus Pituitary gland Reproduction dysfunction Seasonal reproduction This study evaluated by immunohistochemical and Western blot methods, the distribution of two distinct gonadotropin-releasing hormones (GnRHs), corresponding to catfish GnRH (cfGnRH or GnRH1) and chicken-II GnRH (cGnRH-II or GnRH2), in Steindachneridion parahybae females in captivity, focusing these analyses on the reproductive cycle by semi-quantification of optical density (OD). Further, we found that the GnRH neuronal systems co-localized with their respective GnRH-associated peptides (GAPs). A group of neurons immunoreactive (ir) to GnRH1 were identified along the ventral region of the olfactory bulb (vOB) in the telencephalon (vTel) and in the main areas of the diencephalon (especially the medial basal hypothalamus, HBM), including fibers extending into the pituitary gland. In contrast, GnRH2 neurons were confined to the midbrain tegmentum, close to the ventricular surface, without projections to the pituitary gland. Moreover, a cfGAP (GnRH1)-specific band (9 kDa) was identified in the brain and pituitary gland, while a cGAP-II (GnRH2)-specific band (26 kDa) was observed only in the brain extract. During the reproductive cycle, GnRH1-ir presented greater OD values at the vitellogenic and regression stages than at the previtellogenic stage and after artificially induced to spawn. Larger GnRH2-ir neurons were observed during the reproductive cycle, but a higher OD was identified only in the regression stage compared with the other maturation stages. Finally, GnRH1 axons were found to be directed towards the pituitary, and this GnRH type, which is probably the hypophysiotropic form, can contribute to the reproductive dysfunction that occurs in S. parahybae females in captivity, whereas GnRH2 may act as a neuromodulator and/or neurotransmitter. © 2018 Elsevier Inc. 2019 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v273_n_p73_Honji http://hdl.handle.net/20.500.12110/paper_00166480_v273_n_p73_Honji
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic GnRH
Hypothalamus
Pituitary gland
Reproduction dysfunction
Seasonal reproduction
spellingShingle GnRH
Hypothalamus
Pituitary gland
Reproduction dysfunction
Seasonal reproduction
Characterization of the gonadotropin-releasing hormone system in the Neotropical teleost, Steindachneridion parahybae during the annual reproductive cycle in captivity
topic_facet GnRH
Hypothalamus
Pituitary gland
Reproduction dysfunction
Seasonal reproduction
description This study evaluated by immunohistochemical and Western blot methods, the distribution of two distinct gonadotropin-releasing hormones (GnRHs), corresponding to catfish GnRH (cfGnRH or GnRH1) and chicken-II GnRH (cGnRH-II or GnRH2), in Steindachneridion parahybae females in captivity, focusing these analyses on the reproductive cycle by semi-quantification of optical density (OD). Further, we found that the GnRH neuronal systems co-localized with their respective GnRH-associated peptides (GAPs). A group of neurons immunoreactive (ir) to GnRH1 were identified along the ventral region of the olfactory bulb (vOB) in the telencephalon (vTel) and in the main areas of the diencephalon (especially the medial basal hypothalamus, HBM), including fibers extending into the pituitary gland. In contrast, GnRH2 neurons were confined to the midbrain tegmentum, close to the ventricular surface, without projections to the pituitary gland. Moreover, a cfGAP (GnRH1)-specific band (9 kDa) was identified in the brain and pituitary gland, while a cGAP-II (GnRH2)-specific band (26 kDa) was observed only in the brain extract. During the reproductive cycle, GnRH1-ir presented greater OD values at the vitellogenic and regression stages than at the previtellogenic stage and after artificially induced to spawn. Larger GnRH2-ir neurons were observed during the reproductive cycle, but a higher OD was identified only in the regression stage compared with the other maturation stages. Finally, GnRH1 axons were found to be directed towards the pituitary, and this GnRH type, which is probably the hypophysiotropic form, can contribute to the reproductive dysfunction that occurs in S. parahybae females in captivity, whereas GnRH2 may act as a neuromodulator and/or neurotransmitter. © 2018 Elsevier Inc.
title Characterization of the gonadotropin-releasing hormone system in the Neotropical teleost, Steindachneridion parahybae during the annual reproductive cycle in captivity
title_short Characterization of the gonadotropin-releasing hormone system in the Neotropical teleost, Steindachneridion parahybae during the annual reproductive cycle in captivity
title_full Characterization of the gonadotropin-releasing hormone system in the Neotropical teleost, Steindachneridion parahybae during the annual reproductive cycle in captivity
title_fullStr Characterization of the gonadotropin-releasing hormone system in the Neotropical teleost, Steindachneridion parahybae during the annual reproductive cycle in captivity
title_full_unstemmed Characterization of the gonadotropin-releasing hormone system in the Neotropical teleost, Steindachneridion parahybae during the annual reproductive cycle in captivity
title_sort characterization of the gonadotropin-releasing hormone system in the neotropical teleost, steindachneridion parahybae during the annual reproductive cycle in captivity
publishDate 2019
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v273_n_p73_Honji
http://hdl.handle.net/20.500.12110/paper_00166480_v273_n_p73_Honji
_version_ 1768544894711758848

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