Melanin concentrating hormone (MCH) is involved in the regulation of growth hormone in Cichlasoma dimerus (Cichlidae, Teleostei)

Growth hormone (GH) is the main pituitary hormone involved in somatic growth. In fish, the neuroendocrine control of GH is multifactorial due to the interaction of multiple inhibitors and stimulators. Melanin-concentrating hormone (MCH) is a cyclic peptide involved in skin color regulation of fish....

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Detalles Bibliográficos
Autores principales: Cánepa, Maximiliano Martín, Fossati, Mariana, Canosa, Luis Fabián, Somoza, Gustavo Manuel, Vissio, Paula Gabriela
Publicado: 2012
Materias:
Cichlid
Growth hormone
Melanin concentrating hormone
Pituitary
Teleost
complementary DNA
growth hormone
melanin concentrating hormone
animal experiment
animal tissue
article
body growth
body height
body weight
Cichlasoma dimerus
cichlid
controlled study
female
hormonal regulation
hormone action
hormone determination
hormone release
hormone structure
hormone synthesis
hypophysis pars distalis
male
nonhuman
nucleotide sequence
priority journal
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v176_n1_p102_PerezSirkin
http://hdl.handle.net/20.500.12110/paper_00166480_v176_n1_p102_PerezSirkin
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00166480_v176_n1_p102_PerezSirkin
record_format dspace
spelling paper:paper_00166480_v176_n1_p102_PerezSirkin2023-06-08T14:38:19Z Melanin concentrating hormone (MCH) is involved in the regulation of growth hormone in Cichlasoma dimerus (Cichlidae, Teleostei) Cánepa, Maximiliano Martín Fossati, Mariana Canosa, Luis Fabián Somoza, Gustavo Manuel Vissio, Paula Gabriela Cichlid Growth hormone Melanin concentrating hormone Pituitary Teleost complementary DNA growth hormone melanin concentrating hormone animal experiment animal tissue article body growth body height body weight Cichlasoma dimerus cichlid controlled study female hormonal regulation hormone action hormone determination hormone release hormone structure hormone synthesis hypophysis pars distalis male nonhuman nucleotide sequence priority journal Growth hormone (GH) is the main pituitary hormone involved in somatic growth. In fish, the neuroendocrine control of GH is multifactorial due to the interaction of multiple inhibitors and stimulators. Melanin-concentrating hormone (MCH) is a cyclic peptide involved in skin color regulation of fish. In addition, MCH has been related to the regulation of food intake in both mammals and fish. There is only one report presenting evidences on the GH release stimulation by MCH in mammals in experiments in vitro, but there are no data on non-mammals. In the present work, we report for the first time the sequence of MCH and GH cDNA in Cichlasoma dimerus, a freshwater South American cichlid fish. We detected contacts between MCH fibers and GH cells in the proximal pars distalis region of the pituitary gland by double label confocal immunofluorescence indicating a possible functional relationship. Besides, we found that MCH increased GH transcript levels and stimulated GH release in pituitary cultures. Additionally, C. dimerus exposed to a white background had a greater number of MCH neurons with a larger nuclear area and higher levels of MCH transcript than those fish exposed to a black background. Furthermore, fish reared for 3. months in a white background showed a greater body weight and total length compared to those from black background suggesting that MCH might be related to somatic growth in C. dimerus. Our results report for the first time, that MCH is involved in the regulation of the synthesis and release of GH in vitro in C. dimerus, and probably in the fish growth rate. © 2012 Elsevier Inc. Fil:Cánepa, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fossati, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Canosa, L.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Somoza, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vissio, P.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v176_n1_p102_PerezSirkin http://hdl.handle.net/20.500.12110/paper_00166480_v176_n1_p102_PerezSirkin
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Cichlid
Growth hormone
Melanin concentrating hormone
Pituitary
Teleost
complementary DNA
growth hormone
melanin concentrating hormone
animal experiment
animal tissue
article
body growth
body height
body weight
Cichlasoma dimerus
cichlid
controlled study
female
hormonal regulation
hormone action
hormone determination
hormone release
hormone structure
hormone synthesis
hypophysis pars distalis
male
nonhuman
nucleotide sequence
priority journal
spellingShingle Cichlid
Growth hormone
Melanin concentrating hormone
Pituitary
Teleost
complementary DNA
growth hormone
melanin concentrating hormone
animal experiment
animal tissue
article
body growth
body height
body weight
Cichlasoma dimerus
cichlid
controlled study
female
hormonal regulation
hormone action
hormone determination
hormone release
hormone structure
hormone synthesis
hypophysis pars distalis
male
nonhuman
nucleotide sequence
priority journal
Cánepa, Maximiliano Martín
Fossati, Mariana
Canosa, Luis Fabián
Somoza, Gustavo Manuel
Vissio, Paula Gabriela
Melanin concentrating hormone (MCH) is involved in the regulation of growth hormone in Cichlasoma dimerus (Cichlidae, Teleostei)
topic_facet Cichlid
Growth hormone
Melanin concentrating hormone
Pituitary
Teleost
complementary DNA
growth hormone
melanin concentrating hormone
animal experiment
animal tissue
article
body growth
body height
body weight
Cichlasoma dimerus
cichlid
controlled study
female
hormonal regulation
hormone action
hormone determination
hormone release
hormone structure
hormone synthesis
hypophysis pars distalis
male
nonhuman
nucleotide sequence
priority journal
description Growth hormone (GH) is the main pituitary hormone involved in somatic growth. In fish, the neuroendocrine control of GH is multifactorial due to the interaction of multiple inhibitors and stimulators. Melanin-concentrating hormone (MCH) is a cyclic peptide involved in skin color regulation of fish. In addition, MCH has been related to the regulation of food intake in both mammals and fish. There is only one report presenting evidences on the GH release stimulation by MCH in mammals in experiments in vitro, but there are no data on non-mammals. In the present work, we report for the first time the sequence of MCH and GH cDNA in Cichlasoma dimerus, a freshwater South American cichlid fish. We detected contacts between MCH fibers and GH cells in the proximal pars distalis region of the pituitary gland by double label confocal immunofluorescence indicating a possible functional relationship. Besides, we found that MCH increased GH transcript levels and stimulated GH release in pituitary cultures. Additionally, C. dimerus exposed to a white background had a greater number of MCH neurons with a larger nuclear area and higher levels of MCH transcript than those fish exposed to a black background. Furthermore, fish reared for 3. months in a white background showed a greater body weight and total length compared to those from black background suggesting that MCH might be related to somatic growth in C. dimerus. Our results report for the first time, that MCH is involved in the regulation of the synthesis and release of GH in vitro in C. dimerus, and probably in the fish growth rate. © 2012 Elsevier Inc.
author Cánepa, Maximiliano Martín
Fossati, Mariana
Canosa, Luis Fabián
Somoza, Gustavo Manuel
Vissio, Paula Gabriela
author_facet Cánepa, Maximiliano Martín
Fossati, Mariana
Canosa, Luis Fabián
Somoza, Gustavo Manuel
Vissio, Paula Gabriela
author_sort Cánepa, Maximiliano Martín
title Melanin concentrating hormone (MCH) is involved in the regulation of growth hormone in Cichlasoma dimerus (Cichlidae, Teleostei)
title_short Melanin concentrating hormone (MCH) is involved in the regulation of growth hormone in Cichlasoma dimerus (Cichlidae, Teleostei)
title_full Melanin concentrating hormone (MCH) is involved in the regulation of growth hormone in Cichlasoma dimerus (Cichlidae, Teleostei)
title_fullStr Melanin concentrating hormone (MCH) is involved in the regulation of growth hormone in Cichlasoma dimerus (Cichlidae, Teleostei)
title_full_unstemmed Melanin concentrating hormone (MCH) is involved in the regulation of growth hormone in Cichlasoma dimerus (Cichlidae, Teleostei)
title_sort melanin concentrating hormone (mch) is involved in the regulation of growth hormone in cichlasoma dimerus (cichlidae, teleostei)
publishDate 2012
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v176_n1_p102_PerezSirkin
http://hdl.handle.net/20.500.12110/paper_00166480_v176_n1_p102_PerezSirkin
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AT fossatimariana melaninconcentratinghormonemchisinvolvedintheregulationofgrowthhormoneincichlasomadimeruscichlidaeteleostei
AT canosaluisfabian melaninconcentratinghormonemchisinvolvedintheregulationofgrowthhormoneincichlasomadimeruscichlidaeteleostei
AT somozagustavomanuel melaninconcentratinghormonemchisinvolvedintheregulationofgrowthhormoneincichlasomadimeruscichlidaeteleostei
AT vissiopaulagabriela melaninconcentratinghormonemchisinvolvedintheregulationofgrowthhormoneincichlasomadimeruscichlidaeteleostei
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