SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation
Activity of the human long interspersed nuclear elements-1 (LINE-1) retrotransposon occurs mainly in early embryonic development and during hippocampal neurogenesis. SOX-11, a transcription factor relevant to neuronal development, has unknown functions in the control of LINE-1 retrotransposon activi...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v592_n22_p3708_Orqueda http://hdl.handle.net/20.500.12110/paper_00145793_v592_n22_p3708_Orqueda |
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paper:paper_00145793_v592_n22_p3708_Orqueda2023-06-08T14:37:46Z SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation human adipose-derived stem cells LINE-1 neuroblastoma neuronal differentiation SOX-11 DNA LINE1 DNA transcription factor Sox11 unclassified drug 5' untranslated region adipose derived stem cell adipose mesenchymal stem cell Article cell fate controlled study DNA sequence DNA transposition gene knockdown genetic transcription human human cell mesenchymal stem cell nerve cell differentiation priority journal protein DNA interaction retroposon SH-SY5Y cell line upregulation Activity of the human long interspersed nuclear elements-1 (LINE-1) retrotransposon occurs mainly in early embryonic development and during hippocampal neurogenesis. SOX-11, a transcription factor relevant to neuronal development, has unknown functions in the control of LINE-1 retrotransposon activity during neuronal differentiation. To study the dependence of LINE-1 activity on SOX-11 during neuronal differentiation, we induced differentiation of human SH-SY5Y neuroblastoma cells and adult adipose mesenchymal stem cells (hASCs) to a neuronal fate and found increased LINE-1 activity. We also show that SOX-11 protein binding to the LINE-1 promoter is higher in differentiating neuroblastoma cells, while knock-down of SOX-11 inhibits the induction of LINE-1 transcription in differentiating conditions. These results suggest that activation of LINE-1 retrotransposition during neuronal differentiation is mediated by SOX-11. © 2018 Federation of European Biochemical Societies 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v592_n22_p3708_Orqueda http://hdl.handle.net/20.500.12110/paper_00145793_v592_n22_p3708_Orqueda |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
human adipose-derived stem cells LINE-1 neuroblastoma neuronal differentiation SOX-11 DNA LINE1 DNA transcription factor Sox11 unclassified drug 5' untranslated region adipose derived stem cell adipose mesenchymal stem cell Article cell fate controlled study DNA sequence DNA transposition gene knockdown genetic transcription human human cell mesenchymal stem cell nerve cell differentiation priority journal protein DNA interaction retroposon SH-SY5Y cell line upregulation |
| spellingShingle |
human adipose-derived stem cells LINE-1 neuroblastoma neuronal differentiation SOX-11 DNA LINE1 DNA transcription factor Sox11 unclassified drug 5' untranslated region adipose derived stem cell adipose mesenchymal stem cell Article cell fate controlled study DNA sequence DNA transposition gene knockdown genetic transcription human human cell mesenchymal stem cell nerve cell differentiation priority journal protein DNA interaction retroposon SH-SY5Y cell line upregulation SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation |
| topic_facet |
human adipose-derived stem cells LINE-1 neuroblastoma neuronal differentiation SOX-11 DNA LINE1 DNA transcription factor Sox11 unclassified drug 5' untranslated region adipose derived stem cell adipose mesenchymal stem cell Article cell fate controlled study DNA sequence DNA transposition gene knockdown genetic transcription human human cell mesenchymal stem cell nerve cell differentiation priority journal protein DNA interaction retroposon SH-SY5Y cell line upregulation |
| description |
Activity of the human long interspersed nuclear elements-1 (LINE-1) retrotransposon occurs mainly in early embryonic development and during hippocampal neurogenesis. SOX-11, a transcription factor relevant to neuronal development, has unknown functions in the control of LINE-1 retrotransposon activity during neuronal differentiation. To study the dependence of LINE-1 activity on SOX-11 during neuronal differentiation, we induced differentiation of human SH-SY5Y neuroblastoma cells and adult adipose mesenchymal stem cells (hASCs) to a neuronal fate and found increased LINE-1 activity. We also show that SOX-11 protein binding to the LINE-1 promoter is higher in differentiating neuroblastoma cells, while knock-down of SOX-11 inhibits the induction of LINE-1 transcription in differentiating conditions. These results suggest that activation of LINE-1 retrotransposition during neuronal differentiation is mediated by SOX-11. © 2018 Federation of European Biochemical Societies |
| title |
SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation |
| title_short |
SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation |
| title_full |
SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation |
| title_fullStr |
SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation |
| title_full_unstemmed |
SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation |
| title_sort |
sox-11 regulates line-1 retrotransposon activity during neuronal differentiation |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v592_n22_p3708_Orqueda http://hdl.handle.net/20.500.12110/paper_00145793_v592_n22_p3708_Orqueda |
| _version_ |
1768541547229347840 |