Early cell cycle diacylglycerol (DAG) content and protein kinase C (PKC) activity enhancement potentiates prostaglandin F2α (PGF2α) induced mitogenesis in Swiss 3T3 cells

R59022, a diacylglycerol kinase inhibitor, enhances the prostaglandin F2α (PGF2α)-induced diacylglycerol (DAG) synthesis in Swiss 3T3 cells. It also potentiates the PGF2α-mediated protein kinase C (PKC)-dependent 80 kDa protein (80K) phosphorylation and initiation of DNA replication. R 59022 enhance...

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Guardado en:
Detalles Bibliográficos
Publicado: 1993
Materias:
Diacylglycerol kinase
DNA synthesis
Prostaglandin F2α
Protein kinase C
R 59022
6 [2 [4 [(4 fluorophenyl)phenylmethylene] 1 piperidyl]ethyl] 7 methyl 5h thiazolo[3,2 a]pyrimidin 5 one
diacylglycerol
insulin
prostaglandin f2 alpha
protein kinase c
animal cell
article
cell cycle
mitogenesis
mouse
nonhuman
priority journal
3T3 Cells
Animal
Cell Cycle
Cell Division
Diacylglycerol Kinase
Diglycerides
Dinoprost
DNA Replication
Insulin
Mice
Phosphorylation
Phosphotransferases
Protein Kinase C
Pyrimidinones
Support, Non-U.S. Gov't
Thiazoles
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v316_n1_p68_Goin
http://hdl.handle.net/20.500.12110/paper_00145793_v316_n1_p68_Goin
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:R59022, a diacylglycerol kinase inhibitor, enhances the prostaglandin F2α (PGF2α)-induced diacylglycerol (DAG) synthesis in Swiss 3T3 cells. It also potentiates the PGF2α-mediated protein kinase C (PKC)-dependent 80 kDa protein (80K) phosphorylation and initiation of DNA replication. R 59022 enhances the PGF2α mitogenic response by increasing the rate of entry into the S phase. Insulin does not cause 80K phosphorylation, and does not enhance its induction but it potentiates the PGF2α mitogenic response. These results suggest that mitogenically triggered fluctuations in DAG content and PKC activity play a pivotal role in controlling the PGF2α-induced DNA synthesis while insulin acts via a different mechanism. © 1993.

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