Early cell cycle diacylglycerol (DAG) content and protein kinase C (PKC) activity enhancement potentiates prostaglandin F2α (PGF2α) induced mitogenesis in Swiss 3T3 cells

R59022, a diacylglycerol kinase inhibitor, enhances the prostaglandin F2α (PGF2α)-induced diacylglycerol (DAG) synthesis in Swiss 3T3 cells. It also potentiates the PGF2α-mediated protein kinase C (PKC)-dependent 80 kDa protein (80K) phosphorylation and initiation of DNA replication. R 59022 enhance...

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Detalles Bibliográficos
Publicado: 1993
Materias:
Diacylglycerol kinase
DNA synthesis
Prostaglandin F2α
Protein kinase C
R 59022
6 [2 [4 [(4 fluorophenyl)phenylmethylene] 1 piperidyl]ethyl] 7 methyl 5h thiazolo[3,2 a]pyrimidin 5 one
diacylglycerol
insulin
prostaglandin f2 alpha
protein kinase c
animal cell
article
cell cycle
mitogenesis
mouse
nonhuman
priority journal
3T3 Cells
Animal
Cell Cycle
Cell Division
Diacylglycerol Kinase
Diglycerides
Dinoprost
DNA Replication
Insulin
Mice
Phosphorylation
Phosphotransferases
Protein Kinase C
Pyrimidinones
Support, Non-U.S. Gov't
Thiazoles
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v316_n1_p68_Goin
http://hdl.handle.net/20.500.12110/paper_00145793_v316_n1_p68_Goin
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00145793_v316_n1_p68_Goin
record_format dspace
spelling paper:paper_00145793_v316_n1_p68_Goin2023-06-08T14:37:37Z Early cell cycle diacylglycerol (DAG) content and protein kinase C (PKC) activity enhancement potentiates prostaglandin F2α (PGF2α) induced mitogenesis in Swiss 3T3 cells Diacylglycerol kinase DNA synthesis Prostaglandin F2α Protein kinase C R 59022 6 [2 [4 [(4 fluorophenyl)phenylmethylene] 1 piperidyl]ethyl] 7 methyl 5h thiazolo[3,2 a]pyrimidin 5 one diacylglycerol insulin prostaglandin f2 alpha protein kinase c animal cell article cell cycle mitogenesis mouse nonhuman priority journal 3T3 Cells Animal Cell Cycle Cell Division Diacylglycerol Kinase Diglycerides Dinoprost DNA Replication Insulin Mice Phosphorylation Phosphotransferases Protein Kinase C Pyrimidinones Support, Non-U.S. Gov't Thiazoles R59022, a diacylglycerol kinase inhibitor, enhances the prostaglandin F2α (PGF2α)-induced diacylglycerol (DAG) synthesis in Swiss 3T3 cells. It also potentiates the PGF2α-mediated protein kinase C (PKC)-dependent 80 kDa protein (80K) phosphorylation and initiation of DNA replication. R 59022 enhances the PGF2α mitogenic response by increasing the rate of entry into the S phase. Insulin does not cause 80K phosphorylation, and does not enhance its induction but it potentiates the PGF2α mitogenic response. These results suggest that mitogenically triggered fluctuations in DAG content and PKC activity play a pivotal role in controlling the PGF2α-induced DNA synthesis while insulin acts via a different mechanism. © 1993. 1993 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v316_n1_p68_Goin http://hdl.handle.net/20.500.12110/paper_00145793_v316_n1_p68_Goin
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Diacylglycerol kinase
DNA synthesis
Prostaglandin F2α
Protein kinase C
R 59022
6 [2 [4 [(4 fluorophenyl)phenylmethylene] 1 piperidyl]ethyl] 7 methyl 5h thiazolo[3,2 a]pyrimidin 5 one
diacylglycerol
insulin
prostaglandin f2 alpha
protein kinase c
animal cell
article
cell cycle
mitogenesis
mouse
nonhuman
priority journal
3T3 Cells
Animal
Cell Cycle
Cell Division
Diacylglycerol Kinase
Diglycerides
Dinoprost
DNA Replication
Insulin
Mice
Phosphorylation
Phosphotransferases
Protein Kinase C
Pyrimidinones
Support, Non-U.S. Gov't
Thiazoles
spellingShingle Diacylglycerol kinase
DNA synthesis
Prostaglandin F2α
Protein kinase C
R 59022
6 [2 [4 [(4 fluorophenyl)phenylmethylene] 1 piperidyl]ethyl] 7 methyl 5h thiazolo[3,2 a]pyrimidin 5 one
diacylglycerol
insulin
prostaglandin f2 alpha
protein kinase c
animal cell
article
cell cycle
mitogenesis
mouse
nonhuman
priority journal
3T3 Cells
Animal
Cell Cycle
Cell Division
Diacylglycerol Kinase
Diglycerides
Dinoprost
DNA Replication
Insulin
Mice
Phosphorylation
Phosphotransferases
Protein Kinase C
Pyrimidinones
Support, Non-U.S. Gov't
Thiazoles
Early cell cycle diacylglycerol (DAG) content and protein kinase C (PKC) activity enhancement potentiates prostaglandin F2α (PGF2α) induced mitogenesis in Swiss 3T3 cells
topic_facet Diacylglycerol kinase
DNA synthesis
Prostaglandin F2α
Protein kinase C
R 59022
6 [2 [4 [(4 fluorophenyl)phenylmethylene] 1 piperidyl]ethyl] 7 methyl 5h thiazolo[3,2 a]pyrimidin 5 one
diacylglycerol
insulin
prostaglandin f2 alpha
protein kinase c
animal cell
article
cell cycle
mitogenesis
mouse
nonhuman
priority journal
3T3 Cells
Animal
Cell Cycle
Cell Division
Diacylglycerol Kinase
Diglycerides
Dinoprost
DNA Replication
Insulin
Mice
Phosphorylation
Phosphotransferases
Protein Kinase C
Pyrimidinones
Support, Non-U.S. Gov't
Thiazoles
description R59022, a diacylglycerol kinase inhibitor, enhances the prostaglandin F2α (PGF2α)-induced diacylglycerol (DAG) synthesis in Swiss 3T3 cells. It also potentiates the PGF2α-mediated protein kinase C (PKC)-dependent 80 kDa protein (80K) phosphorylation and initiation of DNA replication. R 59022 enhances the PGF2α mitogenic response by increasing the rate of entry into the S phase. Insulin does not cause 80K phosphorylation, and does not enhance its induction but it potentiates the PGF2α mitogenic response. These results suggest that mitogenically triggered fluctuations in DAG content and PKC activity play a pivotal role in controlling the PGF2α-induced DNA synthesis while insulin acts via a different mechanism. © 1993.
title Early cell cycle diacylglycerol (DAG) content and protein kinase C (PKC) activity enhancement potentiates prostaglandin F2α (PGF2α) induced mitogenesis in Swiss 3T3 cells
title_short Early cell cycle diacylglycerol (DAG) content and protein kinase C (PKC) activity enhancement potentiates prostaglandin F2α (PGF2α) induced mitogenesis in Swiss 3T3 cells
title_full Early cell cycle diacylglycerol (DAG) content and protein kinase C (PKC) activity enhancement potentiates prostaglandin F2α (PGF2α) induced mitogenesis in Swiss 3T3 cells
title_fullStr Early cell cycle diacylglycerol (DAG) content and protein kinase C (PKC) activity enhancement potentiates prostaglandin F2α (PGF2α) induced mitogenesis in Swiss 3T3 cells
title_full_unstemmed Early cell cycle diacylglycerol (DAG) content and protein kinase C (PKC) activity enhancement potentiates prostaglandin F2α (PGF2α) induced mitogenesis in Swiss 3T3 cells
title_sort early cell cycle diacylglycerol (dag) content and protein kinase c (pkc) activity enhancement potentiates prostaglandin f2α (pgf2α) induced mitogenesis in swiss 3t3 cells
publishDate 1993
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v316_n1_p68_Goin
http://hdl.handle.net/20.500.12110/paper_00145793_v316_n1_p68_Goin
_version_ 1768543450942144512

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