Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor

ABUIN, G., COUTO, A. S., DE LEDERKREMER, R. M., CASAL, O. L., GALLI, C., COLLI, W., AND ALVES, M. J. M. 1996. Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor. Experimental Parasitology 82, 290-297. Tc-85, an 85-kDa surfac...

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Autores principales:	Couto, Alicia Susana, Muchnik de Lederkremer, Rosa María, Casal de Russo, Olga Liliana
Publicado:	1996
Materias:	Trypanosoma Trypanosoma cruzi glycoprotein inositol membrane protein animal experiment article controlled study high performance liquid chromatography immunoprecipitation nonhuman polyacrylamide gel electrophoresis priority journal thin layer chromatography trypanosoma cruzi trypomastigote Animals Antibodies, Monoclonal Blotting, Western Chromatography, Thin Layer Glycoproteins Glycosylphosphatidylinositols Membrane Proteins Precipitin Tests Protozoan Proteins
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144894_v82_n3_p290_Abuin http://hdl.handle.net/20.500.12110/paper_00144894_v82_n3_p290_Abuin
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00144894_v82_n3_p290_Abuin
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spelling	paper:paper_00144894_v82_n3_p290_Abuin2023-06-08T14:37:24Z Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor Couto, Alicia Susana Muchnik de Lederkremer, Rosa María Casal de Russo, Olga Liliana Trypanosoma Trypanosoma cruzi glycoprotein inositol membrane protein animal experiment article controlled study high performance liquid chromatography immunoprecipitation nonhuman polyacrylamide gel electrophoresis priority journal thin layer chromatography trypanosoma cruzi trypomastigote Animals Antibodies, Monoclonal Blotting, Western Chromatography, Thin Layer Glycoproteins Glycosylphosphatidylinositols Membrane Proteins Precipitin Tests Protozoan Proteins Trypanosoma cruzi ABUIN, G., COUTO, A. S., DE LEDERKREMER, R. M., CASAL, O. L., GALLI, C., COLLI, W., AND ALVES, M. J. M. 1996. Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor. Experimental Parasitology 82, 290-297. Tc-85, an 85-kDa surface glycoprotein specific for the trypomastigote stage of Trypanosoma cruzi, has been implicated in the invasion of host cells by the parasite. Radioactive palmitic acid was incorporated into Tc-85 immunoprecipitated from the culture medium with the H1A10 monoclonal antibody, suggesting that shedding occurs with Tc-85 bearing its GPI anchor. In contrast to the glycoprotein remaining in the parasites, the glycosylphosphatidylinositol moiety in shed Tc-85 is resistant to phosphatidylinositol phospholipase C and becomes susceptible to the enzyme following alkali treatment. An alkylglycerol was identified by thin layer chromatography of an ether extract after the enzymatic reaction. Resistance to cleavage by phospholipase C is due to fatty acid esterification of the inositol residue in shed Tc-85. This is the first example of inositol modification in anchors from a glycoprotein of Trypanosoma cruzi. © 1996 Academic Press, Inc. Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Casal, O.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144894_v82_n3_p290_Abuin http://hdl.handle.net/20.500.12110/paper_00144894_v82_n3_p290_Abuin
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Trypanosoma Trypanosoma cruzi glycoprotein inositol membrane protein animal experiment article controlled study high performance liquid chromatography immunoprecipitation nonhuman polyacrylamide gel electrophoresis priority journal thin layer chromatography trypanosoma cruzi trypomastigote Animals Antibodies, Monoclonal Blotting, Western Chromatography, Thin Layer Glycoproteins Glycosylphosphatidylinositols Membrane Proteins Precipitin Tests Protozoan Proteins Trypanosoma cruzi
spellingShingle	Trypanosoma Trypanosoma cruzi glycoprotein inositol membrane protein animal experiment article controlled study high performance liquid chromatography immunoprecipitation nonhuman polyacrylamide gel electrophoresis priority journal thin layer chromatography trypanosoma cruzi trypomastigote Animals Antibodies, Monoclonal Blotting, Western Chromatography, Thin Layer Glycoproteins Glycosylphosphatidylinositols Membrane Proteins Precipitin Tests Protozoan Proteins Trypanosoma cruzi Couto, Alicia Susana Muchnik de Lederkremer, Rosa María Casal de Russo, Olga Liliana Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor
topic_facet	Trypanosoma Trypanosoma cruzi glycoprotein inositol membrane protein animal experiment article controlled study high performance liquid chromatography immunoprecipitation nonhuman polyacrylamide gel electrophoresis priority journal thin layer chromatography trypanosoma cruzi trypomastigote Animals Antibodies, Monoclonal Blotting, Western Chromatography, Thin Layer Glycoproteins Glycosylphosphatidylinositols Membrane Proteins Precipitin Tests Protozoan Proteins Trypanosoma cruzi
description	ABUIN, G., COUTO, A. S., DE LEDERKREMER, R. M., CASAL, O. L., GALLI, C., COLLI, W., AND ALVES, M. J. M. 1996. Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor. Experimental Parasitology 82, 290-297. Tc-85, an 85-kDa surface glycoprotein specific for the trypomastigote stage of Trypanosoma cruzi, has been implicated in the invasion of host cells by the parasite. Radioactive palmitic acid was incorporated into Tc-85 immunoprecipitated from the culture medium with the H1A10 monoclonal antibody, suggesting that shedding occurs with Tc-85 bearing its GPI anchor. In contrast to the glycoprotein remaining in the parasites, the glycosylphosphatidylinositol moiety in shed Tc-85 is resistant to phosphatidylinositol phospholipase C and becomes susceptible to the enzyme following alkali treatment. An alkylglycerol was identified by thin layer chromatography of an ether extract after the enzymatic reaction. Resistance to cleavage by phospholipase C is due to fatty acid esterification of the inositol residue in shed Tc-85. This is the first example of inositol modification in anchors from a glycoprotein of Trypanosoma cruzi. © 1996 Academic Press, Inc.
author	Couto, Alicia Susana Muchnik de Lederkremer, Rosa María Casal de Russo, Olga Liliana
author_facet	Couto, Alicia Susana Muchnik de Lederkremer, Rosa María Casal de Russo, Olga Liliana
author_sort	Couto, Alicia Susana
title	Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor
title_short	Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor
title_full	Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor
title_fullStr	Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor
title_full_unstemmed	Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor
title_sort	trypanosoma cruzi: the tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor
publishDate	1996
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144894_v82_n3_p290_Abuin http://hdl.handle.net/20.500.12110/paper_00144894_v82_n3_p290_Abuin
work_keys_str_mv	AT coutoaliciasusana trypanosomacruzithetc85surfaceglycoproteinshedbytrypomastigotesbearsamodifiedglycosylphosphatidylinositolanchor AT muchnikdelederkremerrosamaria trypanosomacruzithetc85surfaceglycoproteinshedbytrypomastigotesbearsamodifiedglycosylphosphatidylinositolanchor AT casalderussoolgaliliana trypanosomacruzithetc85surfaceglycoproteinshedbytrypomastigotesbearsamodifiedglycosylphosphatidylinositolanchor
_version_	1768544668981657600

Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor

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