Trypanosoma cruzi: Antibodies to a MAP-like protein in chronic chagas' disease cross-react with mammalian cytoskeleton
Trypanosoma cruzi λgtll library from epimastogote derived mRNA was screened with human chagasic sera or sera from chronically infected mice. Strong reactive recombinants were detected with both sera. Two recombinant clones were studied in more detail and shown to be composed of the same 114-bp repet...
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1991
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144894_v73_n4_p451_Kerner http://hdl.handle.net/20.500.12110/paper_00144894_v73_n4_p451_Kerner |
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paper:paper_00144894_v73_n4_p451_Kerner2023-06-08T14:37:24Z Trypanosoma cruzi: Antibodies to a MAP-like protein in chronic chagas' disease cross-react with mammalian cytoskeleton cDNA library Chagas' disease Cytoskeleton Immunoscreening Molecular mimicry Protozoa Trypanosoma cruzi MAP-like antigen autoantibody hybrid protein microtubule associated protein microtubule associated protein 5 Microtubule Associated Proteins microtubule-associated protein 1B parasite antigen protozoon antibody amino acid sequence animal article Chagas disease chronic disease cross reaction cytoskeleton immunology molecular genetics mouse nucleotide repeat nucleotide sequence Amino Acid Sequence Animal Antibodies, Protozoan Antigens, Protozoan Autoantibodies Base Sequence Chagas Disease Chronic Disease Cross Reactions Cytoskeleton Mice Microtubule-Associated Proteins Molecular Sequence Data Recombinant Fusion Proteins Repetitive Sequences, Nucleic Acid Support, Non-U.S. Gov't Trypanosoma cruzi λgtll library from epimastogote derived mRNA was screened with human chagasic sera or sera from chronically infected mice. Strong reactive recombinants were detected with both sera. Two recombinant clones were studied in more detail and shown to be composed of the same 114-bp repetitive sequence coding for a 38 amino acid repetition. This repetition is the same size and shares >60% homology with the reported T. brucei microtubule associated protein (MAP) p320. The insert of one of these clones, K1-7 (228 bp), was subcloned into pMSgt11 and the soluble recombinant polypeptide expressed. Antibodies against the K1-7 fusion polypeptide recognized a major 110-kDa band from Cytoskeleton. Anti K1-7 monospecific antibodies detected several cytoskeletal proteins from 3T3 fibroblasts and bovine brain microtubule preparations. Reciprocally, anti-MAP1b monoclonal antibodies raised against bovine brain microtubule reacted with the K1-7 polypeptide on Western blots. The protein identified by K1-7 antibodies may be one of the parasite molecules associated to molecular mimicry. © 1991. 1991 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144894_v73_n4_p451_Kerner http://hdl.handle.net/20.500.12110/paper_00144894_v73_n4_p451_Kerner |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cDNA library Chagas' disease Cytoskeleton Immunoscreening Molecular mimicry Protozoa Trypanosoma cruzi MAP-like antigen autoantibody hybrid protein microtubule associated protein microtubule associated protein 5 Microtubule Associated Proteins microtubule-associated protein 1B parasite antigen protozoon antibody amino acid sequence animal article Chagas disease chronic disease cross reaction cytoskeleton immunology molecular genetics mouse nucleotide repeat nucleotide sequence Amino Acid Sequence Animal Antibodies, Protozoan Antigens, Protozoan Autoantibodies Base Sequence Chagas Disease Chronic Disease Cross Reactions Cytoskeleton Mice Microtubule-Associated Proteins Molecular Sequence Data Recombinant Fusion Proteins Repetitive Sequences, Nucleic Acid Support, Non-U.S. Gov't |
| spellingShingle |
cDNA library Chagas' disease Cytoskeleton Immunoscreening Molecular mimicry Protozoa Trypanosoma cruzi MAP-like antigen autoantibody hybrid protein microtubule associated protein microtubule associated protein 5 Microtubule Associated Proteins microtubule-associated protein 1B parasite antigen protozoon antibody amino acid sequence animal article Chagas disease chronic disease cross reaction cytoskeleton immunology molecular genetics mouse nucleotide repeat nucleotide sequence Amino Acid Sequence Animal Antibodies, Protozoan Antigens, Protozoan Autoantibodies Base Sequence Chagas Disease Chronic Disease Cross Reactions Cytoskeleton Mice Microtubule-Associated Proteins Molecular Sequence Data Recombinant Fusion Proteins Repetitive Sequences, Nucleic Acid Support, Non-U.S. Gov't Trypanosoma cruzi: Antibodies to a MAP-like protein in chronic chagas' disease cross-react with mammalian cytoskeleton |
| topic_facet |
cDNA library Chagas' disease Cytoskeleton Immunoscreening Molecular mimicry Protozoa Trypanosoma cruzi MAP-like antigen autoantibody hybrid protein microtubule associated protein microtubule associated protein 5 Microtubule Associated Proteins microtubule-associated protein 1B parasite antigen protozoon antibody amino acid sequence animal article Chagas disease chronic disease cross reaction cytoskeleton immunology molecular genetics mouse nucleotide repeat nucleotide sequence Amino Acid Sequence Animal Antibodies, Protozoan Antigens, Protozoan Autoantibodies Base Sequence Chagas Disease Chronic Disease Cross Reactions Cytoskeleton Mice Microtubule-Associated Proteins Molecular Sequence Data Recombinant Fusion Proteins Repetitive Sequences, Nucleic Acid Support, Non-U.S. Gov't |
| description |
Trypanosoma cruzi λgtll library from epimastogote derived mRNA was screened with human chagasic sera or sera from chronically infected mice. Strong reactive recombinants were detected with both sera. Two recombinant clones were studied in more detail and shown to be composed of the same 114-bp repetitive sequence coding for a 38 amino acid repetition. This repetition is the same size and shares >60% homology with the reported T. brucei microtubule associated protein (MAP) p320. The insert of one of these clones, K1-7 (228 bp), was subcloned into pMSgt11 and the soluble recombinant polypeptide expressed. Antibodies against the K1-7 fusion polypeptide recognized a major 110-kDa band from Cytoskeleton. Anti K1-7 monospecific antibodies detected several cytoskeletal proteins from 3T3 fibroblasts and bovine brain microtubule preparations. Reciprocally, anti-MAP1b monoclonal antibodies raised against bovine brain microtubule reacted with the K1-7 polypeptide on Western blots. The protein identified by K1-7 antibodies may be one of the parasite molecules associated to molecular mimicry. © 1991. |
| title |
Trypanosoma cruzi: Antibodies to a MAP-like protein in chronic chagas' disease cross-react with mammalian cytoskeleton |
| title_short |
Trypanosoma cruzi: Antibodies to a MAP-like protein in chronic chagas' disease cross-react with mammalian cytoskeleton |
| title_full |
Trypanosoma cruzi: Antibodies to a MAP-like protein in chronic chagas' disease cross-react with mammalian cytoskeleton |
| title_fullStr |
Trypanosoma cruzi: Antibodies to a MAP-like protein in chronic chagas' disease cross-react with mammalian cytoskeleton |
| title_full_unstemmed |
Trypanosoma cruzi: Antibodies to a MAP-like protein in chronic chagas' disease cross-react with mammalian cytoskeleton |
| title_sort |
trypanosoma cruzi: antibodies to a map-like protein in chronic chagas' disease cross-react with mammalian cytoskeleton |
| publishDate |
1991 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144894_v73_n4_p451_Kerner http://hdl.handle.net/20.500.12110/paper_00144894_v73_n4_p451_Kerner |
| _version_ |
1768543928955437056 |