Prominently decreased hippocampal neurogenesis in a spontaneous model of type 1 diabetes, the nonobese diabetic mouse
In human diabetes, degenerative and functional disorders of the central nervous system, including depression, are common findings. Defective dentate gyrus (DG) neurogenesis is associated with affective-related disorders and depression. We previously demonstrated reduced DG neurogenesis in a pharmaco...
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2008
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144886_v210_n2_p359_Beauquis http://hdl.handle.net/20.500.12110/paper_00144886_v210_n2_p359_Beauquis |
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paper:paper_00144886_v210_n2_p359_Beauquis2023-06-08T14:37:21Z Prominently decreased hippocampal neurogenesis in a spontaneous model of type 1 diabetes, the nonobese diabetic mouse BrdU Dentate gyrus neurogenesis GFAP NeuN NOD mouse Type 1 diabetes mellitus broxuridine corticosterone DNA streptozocin animal cell animal experiment animal model animal tissue article autoimmunity cell proliferation cell survival central nervous system disease central nervous system function confocal microscopy controlled study dentate gyrus depression female hippocampus hypophysis adrenal system inflammation insulin dependent diabetes mellitus mood disorder mouse nonhuman nonobese diabetic mouse phenotype priority journal streptozocin diabetes Age Factors Analysis of Variance Animals Bromodeoxyuridine Cell Count Cell Proliferation Corticosterone Diabetes Mellitus, Type 1 Disease Models, Animal Female Glial Fibrillary Acidic Protein Hippocampus Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred NOD Neurons Phosphopyruvate Hydratase In human diabetes, degenerative and functional disorders of the central nervous system, including depression, are common findings. Defective dentate gyrus (DG) neurogenesis is associated with affective-related disorders and depression. We previously demonstrated reduced DG neurogenesis in a pharmacological type 1 diabetes model, the streptozotocin (STZ)-treated mouse. Here, we explored DG neurogenesis in a spontaneous T1D model, the nonobese diabetic (NOD) mouse, at prediabetic and diabetic stages. Cell proliferation was assessed in the DG of 5, 8 and 12-week-old control C57BL/6 and BALB/c strains and NOD mice, killed 2 h after bromodeoxyuridine (BrdU) administration. Survival of the newly generated cells was studied in 15-week-old animals that were killed 21 days after BrdU injection. The number of proliferative BrdU-positive cells in the DG was, regardless of age, constantly and significantly lower in NOD than in control strains, showing the presence of hippocampal alterations far before clinical diabetes onset in NOD mice. Diabetes also strongly decreased cell survival in NOD DG. However, cell phenotype proportion, as assessed by co-localization with neuronal or glial markers and confocal microscopy, was not modified. Hippocampal neurogenesis is strongly diminished in the spontaneous NOD model, like in the STZ model. Notably, NOD hippocampal DG cell proliferation defect takes place during the prediabetic stage. Whether this early alteration might result, in this autoimmune strain, from hypothalamo-pituitary adrenal axis alterations and/or ongoing brain inflammatory process sharing many characteristics of aging is discussed and deserves further investigation. © 2007 Elsevier Inc. All rights reserved. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144886_v210_n2_p359_Beauquis http://hdl.handle.net/20.500.12110/paper_00144886_v210_n2_p359_Beauquis |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
BrdU Dentate gyrus neurogenesis GFAP NeuN NOD mouse Type 1 diabetes mellitus broxuridine corticosterone DNA streptozocin animal cell animal experiment animal model animal tissue article autoimmunity cell proliferation cell survival central nervous system disease central nervous system function confocal microscopy controlled study dentate gyrus depression female hippocampus hypophysis adrenal system inflammation insulin dependent diabetes mellitus mood disorder mouse nonhuman nonobese diabetic mouse phenotype priority journal streptozocin diabetes Age Factors Analysis of Variance Animals Bromodeoxyuridine Cell Count Cell Proliferation Corticosterone Diabetes Mellitus, Type 1 Disease Models, Animal Female Glial Fibrillary Acidic Protein Hippocampus Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred NOD Neurons Phosphopyruvate Hydratase |
| spellingShingle |
BrdU Dentate gyrus neurogenesis GFAP NeuN NOD mouse Type 1 diabetes mellitus broxuridine corticosterone DNA streptozocin animal cell animal experiment animal model animal tissue article autoimmunity cell proliferation cell survival central nervous system disease central nervous system function confocal microscopy controlled study dentate gyrus depression female hippocampus hypophysis adrenal system inflammation insulin dependent diabetes mellitus mood disorder mouse nonhuman nonobese diabetic mouse phenotype priority journal streptozocin diabetes Age Factors Analysis of Variance Animals Bromodeoxyuridine Cell Count Cell Proliferation Corticosterone Diabetes Mellitus, Type 1 Disease Models, Animal Female Glial Fibrillary Acidic Protein Hippocampus Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred NOD Neurons Phosphopyruvate Hydratase Prominently decreased hippocampal neurogenesis in a spontaneous model of type 1 diabetes, the nonobese diabetic mouse |
| topic_facet |
BrdU Dentate gyrus neurogenesis GFAP NeuN NOD mouse Type 1 diabetes mellitus broxuridine corticosterone DNA streptozocin animal cell animal experiment animal model animal tissue article autoimmunity cell proliferation cell survival central nervous system disease central nervous system function confocal microscopy controlled study dentate gyrus depression female hippocampus hypophysis adrenal system inflammation insulin dependent diabetes mellitus mood disorder mouse nonhuman nonobese diabetic mouse phenotype priority journal streptozocin diabetes Age Factors Analysis of Variance Animals Bromodeoxyuridine Cell Count Cell Proliferation Corticosterone Diabetes Mellitus, Type 1 Disease Models, Animal Female Glial Fibrillary Acidic Protein Hippocampus Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred NOD Neurons Phosphopyruvate Hydratase |
| description |
In human diabetes, degenerative and functional disorders of the central nervous system, including depression, are common findings. Defective dentate gyrus (DG) neurogenesis is associated with affective-related disorders and depression. We previously demonstrated reduced DG neurogenesis in a pharmacological type 1 diabetes model, the streptozotocin (STZ)-treated mouse. Here, we explored DG neurogenesis in a spontaneous T1D model, the nonobese diabetic (NOD) mouse, at prediabetic and diabetic stages. Cell proliferation was assessed in the DG of 5, 8 and 12-week-old control C57BL/6 and BALB/c strains and NOD mice, killed 2 h after bromodeoxyuridine (BrdU) administration. Survival of the newly generated cells was studied in 15-week-old animals that were killed 21 days after BrdU injection. The number of proliferative BrdU-positive cells in the DG was, regardless of age, constantly and significantly lower in NOD than in control strains, showing the presence of hippocampal alterations far before clinical diabetes onset in NOD mice. Diabetes also strongly decreased cell survival in NOD DG. However, cell phenotype proportion, as assessed by co-localization with neuronal or glial markers and confocal microscopy, was not modified. Hippocampal neurogenesis is strongly diminished in the spontaneous NOD model, like in the STZ model. Notably, NOD hippocampal DG cell proliferation defect takes place during the prediabetic stage. Whether this early alteration might result, in this autoimmune strain, from hypothalamo-pituitary adrenal axis alterations and/or ongoing brain inflammatory process sharing many characteristics of aging is discussed and deserves further investigation. © 2007 Elsevier Inc. All rights reserved. |
| title |
Prominently decreased hippocampal neurogenesis in a spontaneous model of type 1 diabetes, the nonobese diabetic mouse |
| title_short |
Prominently decreased hippocampal neurogenesis in a spontaneous model of type 1 diabetes, the nonobese diabetic mouse |
| title_full |
Prominently decreased hippocampal neurogenesis in a spontaneous model of type 1 diabetes, the nonobese diabetic mouse |
| title_fullStr |
Prominently decreased hippocampal neurogenesis in a spontaneous model of type 1 diabetes, the nonobese diabetic mouse |
| title_full_unstemmed |
Prominently decreased hippocampal neurogenesis in a spontaneous model of type 1 diabetes, the nonobese diabetic mouse |
| title_sort |
prominently decreased hippocampal neurogenesis in a spontaneous model of type 1 diabetes, the nonobese diabetic mouse |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144886_v210_n2_p359_Beauquis http://hdl.handle.net/20.500.12110/paper_00144886_v210_n2_p359_Beauquis |
| _version_ |
1768543495583170560 |