Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer

Deciphering molecular pathways involved in the early steps of prostate oncogenesis requires both in vitro and in vivo models derived from human primary tumors. However the few recognized models of human prostate epithelial cancer originate from metastases. To date, very few models are proposed from...

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Publicado:	2011
Materias:	Basal epithelial cells Gene expression profiling Prostatic neoplasms Tumor cells cultured Tumor stem cells 2 methylacyl coenzyme A racemase cytokeratin 14 cytokeratin 5 octamer transcription factor 4 prostate specific membrane antigen protein p53 sonic hedgehog protein telomerase very late activation antigen 2 adult animal cell animal experiment animal tissue article cancer cell culture cancer growth cancer stem cell case report cell structure controlled study enzyme activity epithelium cell extracellular matrix gene mutation human human cell IGR CaP1 cell male mouse nonhuman priority journal prostate cancer protein expression signal transduction tetraploidy Animalia Erinaceidae Mus Mus musculus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v317_n3_p262_Chauchereau http://hdl.handle.net/20.500.12110/paper_00144827_v317_n3_p262_Chauchereau
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00144827_v317_n3_p262_Chauchereau
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spelling	paper:paper_00144827_v317_n3_p262_Chauchereau2023-06-08T14:37:18Z Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer Basal epithelial cells Gene expression profiling Prostatic neoplasms Tumor cells cultured Tumor stem cells 2 methylacyl coenzyme A racemase cytokeratin 14 cytokeratin 5 octamer transcription factor 4 prostate specific membrane antigen protein p53 sonic hedgehog protein telomerase very late activation antigen 2 adult animal cell animal experiment animal tissue article cancer cell culture cancer growth cancer stem cell case report cell structure controlled study enzyme activity epithelium cell extracellular matrix gene mutation human human cell IGR CaP1 cell male mouse nonhuman priority journal prostate cancer protein expression signal transduction tetraploidy Animalia Erinaceidae Mus Mus musculus Deciphering molecular pathways involved in the early steps of prostate oncogenesis requires both in vitro and in vivo models derived from human primary tumors. However the few recognized models of human prostate epithelial cancer originate from metastases. To date, very few models are proposed from primary tumors and immortalizing normal human prostate cells does not recapitulate the natural history of the disease. By culturing human prostate primary tumor cells onto human epithelial extra-cellular matrix, we successfully selected a new prostate cancer cell line, IGR-CaP1, and clonally-derived subclones. IGR-CaP1 cells, that harbor a tetraploid karyotype, high telomerase activity and mutated TP53, rapidly induced subcutaneous xenografts in nude mice. Furthermore, IGR-CaP1 cell lines, all exhibiting negativity for the androgen receptor and PSA, express the specific prostate markers alpha-methylacyl-CoA racemase and a low level of the prostate-specific membrane antigen PSMA, along with the prostate basal epithelial markers CK5 and CK14. More importantly, these clones express high CD44, CD133, and CXCR4 levels associated with high expression of α2β1-integrin and Oct4 which are reported to be prostate cancer stemness markers. RT-PCR data also revealed high activation of the Sonic Hedgehog signalling pathway in these cells. Additionally, the IGR-CaP1 cells possess a 3D sphere-forming ability and a renewal capacity by maintaining their CSC potential after xenografting in mice. As a result, the hormone-independent IGR-CaP1 cellular clones exhibit the original features of both basal prostate tissue and cancer stemness. Tumorigenic IGR-CaP1 clones constitute invaluable human models for studying prostate cancer progression and drug assessment in vitro as well as in animals specifically for developing new therapeutic approaches targeting prostate cancer stem cells. © 2010 Elsevier Inc. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v317_n3_p262_Chauchereau http://hdl.handle.net/20.500.12110/paper_00144827_v317_n3_p262_Chauchereau
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Basal epithelial cells Gene expression profiling Prostatic neoplasms Tumor cells cultured Tumor stem cells 2 methylacyl coenzyme A racemase cytokeratin 14 cytokeratin 5 octamer transcription factor 4 prostate specific membrane antigen protein p53 sonic hedgehog protein telomerase very late activation antigen 2 adult animal cell animal experiment animal tissue article cancer cell culture cancer growth cancer stem cell case report cell structure controlled study enzyme activity epithelium cell extracellular matrix gene mutation human human cell IGR CaP1 cell male mouse nonhuman priority journal prostate cancer protein expression signal transduction tetraploidy Animalia Erinaceidae Mus Mus musculus
spellingShingle	Basal epithelial cells Gene expression profiling Prostatic neoplasms Tumor cells cultured Tumor stem cells 2 methylacyl coenzyme A racemase cytokeratin 14 cytokeratin 5 octamer transcription factor 4 prostate specific membrane antigen protein p53 sonic hedgehog protein telomerase very late activation antigen 2 adult animal cell animal experiment animal tissue article cancer cell culture cancer growth cancer stem cell case report cell structure controlled study enzyme activity epithelium cell extracellular matrix gene mutation human human cell IGR CaP1 cell male mouse nonhuman priority journal prostate cancer protein expression signal transduction tetraploidy Animalia Erinaceidae Mus Mus musculus Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer
topic_facet	Basal epithelial cells Gene expression profiling Prostatic neoplasms Tumor cells cultured Tumor stem cells 2 methylacyl coenzyme A racemase cytokeratin 14 cytokeratin 5 octamer transcription factor 4 prostate specific membrane antigen protein p53 sonic hedgehog protein telomerase very late activation antigen 2 adult animal cell animal experiment animal tissue article cancer cell culture cancer growth cancer stem cell case report cell structure controlled study enzyme activity epithelium cell extracellular matrix gene mutation human human cell IGR CaP1 cell male mouse nonhuman priority journal prostate cancer protein expression signal transduction tetraploidy Animalia Erinaceidae Mus Mus musculus
description	Deciphering molecular pathways involved in the early steps of prostate oncogenesis requires both in vitro and in vivo models derived from human primary tumors. However the few recognized models of human prostate epithelial cancer originate from metastases. To date, very few models are proposed from primary tumors and immortalizing normal human prostate cells does not recapitulate the natural history of the disease. By culturing human prostate primary tumor cells onto human epithelial extra-cellular matrix, we successfully selected a new prostate cancer cell line, IGR-CaP1, and clonally-derived subclones. IGR-CaP1 cells, that harbor a tetraploid karyotype, high telomerase activity and mutated TP53, rapidly induced subcutaneous xenografts in nude mice. Furthermore, IGR-CaP1 cell lines, all exhibiting negativity for the androgen receptor and PSA, express the specific prostate markers alpha-methylacyl-CoA racemase and a low level of the prostate-specific membrane antigen PSMA, along with the prostate basal epithelial markers CK5 and CK14. More importantly, these clones express high CD44, CD133, and CXCR4 levels associated with high expression of α2β1-integrin and Oct4 which are reported to be prostate cancer stemness markers. RT-PCR data also revealed high activation of the Sonic Hedgehog signalling pathway in these cells. Additionally, the IGR-CaP1 cells possess a 3D sphere-forming ability and a renewal capacity by maintaining their CSC potential after xenografting in mice. As a result, the hormone-independent IGR-CaP1 cellular clones exhibit the original features of both basal prostate tissue and cancer stemness. Tumorigenic IGR-CaP1 clones constitute invaluable human models for studying prostate cancer progression and drug assessment in vitro as well as in animals specifically for developing new therapeutic approaches targeting prostate cancer stem cells. © 2010 Elsevier Inc.
title	Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer
title_short	Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer
title_full	Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer
title_fullStr	Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer
title_full_unstemmed	Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer
title_sort	stemness markers characterize igr-cap1, a new cell line derived from primary epithelial prostate cancer
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v317_n3_p262_Chauchereau http://hdl.handle.net/20.500.12110/paper_00144827_v317_n3_p262_Chauchereau
_version_	1768544209765138432

Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer

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