TNF-alpha-induced apoptosis is prevented by erythropoietin treatment on SH-SY5Y cells
The growth factor erythropoietin (Epo) has shown neuronal protective action in addition to its well known proerythroid activity. Furthermore, Epo has dealt with cellular inflammation by inhibiting the expression of several proinflammatory cytokines, such as IL-1 and TNF-α. The action of TNF can have...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v315_n3_p419_Pregi http://hdl.handle.net/20.500.12110/paper_00144827_v315_n3_p419_Pregi |
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paper:paper_00144827_v315_n3_p419_Pregi2023-06-08T14:37:17Z TNF-alpha-induced apoptosis is prevented by erythropoietin treatment on SH-SY5Y cells Pregi, Nicolás Wenker, Shirley Denise Vittori, Daniela Cecilia Pérez Leirós, Claudia Nesse, Alcira Beatriz Apoptosis Erythropoietin Inflammation Neuroprotection NF-κB SH-SY5Y cells TNF-α caspase erythropoietin Janus kinase phosphatidylinositol 3 kinase STAT protein tumor necrosis factor alpha tumor necrosis factor receptor 1 apoptosis article cell death cell line controlled study enzyme activation enzyme activity human human cell neuroprotection priority journal protein transport The growth factor erythropoietin (Epo) has shown neuronal protective action in addition to its well known proerythroid activity. Furthermore, Epo has dealt with cellular inflammation by inhibiting the expression of several proinflammatory cytokines, such as IL-1 and TNF-α. The action of TNF can have both apoptotic and antiapoptotic consequences due to altered balance between different cell signalling pathways. This work has focused on the apoptotic effects of this cytokine and the potential protective action of Epo. The model we used was neuroblastoma SH-SY5Y cells cultured in the presence of 25 ng/ml TNF-α or pretreated with 25 U/ml Epo for 12 h before the addition of TNF-α. Apoptosis was evaluated by differential cell count after Hoechst staining, analysis of DNA ladder pattern, and measurement of caspase activity. Despite its ability to induce NF-κB nuclear translocation, TNF-α induced cell death, which was found to be associated to upregulation of TNF Receptor 1 expression. On the other hand, cells activated by Epo became resistant to cell death. Prevention of death receptor upregulation and caspase activation may explain this antiapoptotic effect of Epo, which may be also favoured by the induction of a higher expression of protective factors, such as Bcl-2 and NF-κB, through mechanisms involving Jak/STAT and PI3K signalling pathways. © 2008 Elsevier Inc. All rights reserved. Fil:Pregi, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wenker, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vittori, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Leirós, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nesse, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v315_n3_p419_Pregi http://hdl.handle.net/20.500.12110/paper_00144827_v315_n3_p419_Pregi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Apoptosis Erythropoietin Inflammation Neuroprotection NF-κB SH-SY5Y cells TNF-α caspase erythropoietin Janus kinase phosphatidylinositol 3 kinase STAT protein tumor necrosis factor alpha tumor necrosis factor receptor 1 apoptosis article cell death cell line controlled study enzyme activation enzyme activity human human cell neuroprotection priority journal protein transport |
| spellingShingle |
Apoptosis Erythropoietin Inflammation Neuroprotection NF-κB SH-SY5Y cells TNF-α caspase erythropoietin Janus kinase phosphatidylinositol 3 kinase STAT protein tumor necrosis factor alpha tumor necrosis factor receptor 1 apoptosis article cell death cell line controlled study enzyme activation enzyme activity human human cell neuroprotection priority journal protein transport Pregi, Nicolás Wenker, Shirley Denise Vittori, Daniela Cecilia Pérez Leirós, Claudia Nesse, Alcira Beatriz TNF-alpha-induced apoptosis is prevented by erythropoietin treatment on SH-SY5Y cells |
| topic_facet |
Apoptosis Erythropoietin Inflammation Neuroprotection NF-κB SH-SY5Y cells TNF-α caspase erythropoietin Janus kinase phosphatidylinositol 3 kinase STAT protein tumor necrosis factor alpha tumor necrosis factor receptor 1 apoptosis article cell death cell line controlled study enzyme activation enzyme activity human human cell neuroprotection priority journal protein transport |
| description |
The growth factor erythropoietin (Epo) has shown neuronal protective action in addition to its well known proerythroid activity. Furthermore, Epo has dealt with cellular inflammation by inhibiting the expression of several proinflammatory cytokines, such as IL-1 and TNF-α. The action of TNF can have both apoptotic and antiapoptotic consequences due to altered balance between different cell signalling pathways. This work has focused on the apoptotic effects of this cytokine and the potential protective action of Epo. The model we used was neuroblastoma SH-SY5Y cells cultured in the presence of 25 ng/ml TNF-α or pretreated with 25 U/ml Epo for 12 h before the addition of TNF-α. Apoptosis was evaluated by differential cell count after Hoechst staining, analysis of DNA ladder pattern, and measurement of caspase activity. Despite its ability to induce NF-κB nuclear translocation, TNF-α induced cell death, which was found to be associated to upregulation of TNF Receptor 1 expression. On the other hand, cells activated by Epo became resistant to cell death. Prevention of death receptor upregulation and caspase activation may explain this antiapoptotic effect of Epo, which may be also favoured by the induction of a higher expression of protective factors, such as Bcl-2 and NF-κB, through mechanisms involving Jak/STAT and PI3K signalling pathways. © 2008 Elsevier Inc. All rights reserved. |
| author |
Pregi, Nicolás Wenker, Shirley Denise Vittori, Daniela Cecilia Pérez Leirós, Claudia Nesse, Alcira Beatriz |
| author_facet |
Pregi, Nicolás Wenker, Shirley Denise Vittori, Daniela Cecilia Pérez Leirós, Claudia Nesse, Alcira Beatriz |
| author_sort |
Pregi, Nicolás |
| title |
TNF-alpha-induced apoptosis is prevented by erythropoietin treatment on SH-SY5Y cells |
| title_short |
TNF-alpha-induced apoptosis is prevented by erythropoietin treatment on SH-SY5Y cells |
| title_full |
TNF-alpha-induced apoptosis is prevented by erythropoietin treatment on SH-SY5Y cells |
| title_fullStr |
TNF-alpha-induced apoptosis is prevented by erythropoietin treatment on SH-SY5Y cells |
| title_full_unstemmed |
TNF-alpha-induced apoptosis is prevented by erythropoietin treatment on SH-SY5Y cells |
| title_sort |
tnf-alpha-induced apoptosis is prevented by erythropoietin treatment on sh-sy5y cells |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v315_n3_p419_Pregi http://hdl.handle.net/20.500.12110/paper_00144827_v315_n3_p419_Pregi |
| work_keys_str_mv |
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| _version_ |
1768542721251737600 |