Binding and effects of catecholestrogens on adenylate cyclase activity, and adrenoceptors, benzodiazepine and GABA receptors in guinea-pig hypothalamic membranes
Catecholestrogen (CE) binding to guinea-pig hypothalamic membranes was assessed by using [3H]2-hydroxyestrone (2-OHE2) as a ligand. Binding was maximal at pH 7.4 and 37°C, and after 10 min incubations. A high affinity binding site with dissociation constant (KD) = 0.20 ± 0.02 nM and site concentrati...
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1986
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v129_n1-2_p1_Etchegoyen http://hdl.handle.net/20.500.12110/paper_00142999_v129_n1-2_p1_Etchegoyen |
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