Augmented thromboxane generation by mesenteric arteries from pancreatectomized diabetic dogs is coincident with the vascular tone enhancement evoked by Na arachidonate and prostacyclin
We studied the relationships between acrachidonic acid (AA) metabolism and contractile responses to Na arachidonate (NaA) and the prostaglandins (PGs) in mesenteric arteries isolated from sham-operated and totally pancreatectomized dogs. PGE2 and PGF2α produced a similar dose-dependent relaxation of...
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1984
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v103_n3-4_p211_SterinBorda http://hdl.handle.net/20.500.12110/paper_00142999_v103_n3-4_p211_SterinBorda |
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paper:paper_00142999_v103_n3-4_p211_SterinBorda |
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paper:paper_00142999_v103_n3-4_p211_SterinBorda2023-06-08T14:36:49Z Augmented thromboxane generation by mesenteric arteries from pancreatectomized diabetic dogs is coincident with the vascular tone enhancement evoked by Na arachidonate and prostacyclin 6-oxo-PGF1α Arachidonic acid Arteries Cyclooxygenase Diabetes Insulin Lipoxygenase Mesenteric vessels Pancreatectomized dogs Prostacyclin Prostaglandins Thromboxane Vasoconstriction Vasodilation 5,8,11,14 icosatetraynoic acid acetylsalicylic acid arachidonic acid c 14 haloperidol imidazole indometacin insulin nordihydroguaiaretic acid phentolamine phentolamine mesylate prostacyclin prostaglandin synthase radioisotope thromboxane tranylcypromine unclassified drug animal model article diabetes mellitus dog drug efficacy endocrine system mesenteric artery nonhuman pancreas pancreas resection peripheral vascular system Animals Arachidonic Acid Arachidonic Acids Diabetes Mellitus, Experimental Dinoprost Dinoprostone Dogs Epoprostenol Mesenteric Arteries Muscle Tonus Muscle, Smooth, Vascular Pancreatectomy Prostaglandins Prostaglandins E Prostaglandins F Thromboxanes Vasoconstriction We studied the relationships between acrachidonic acid (AA) metabolism and contractile responses to Na arachidonate (NaA) and the prostaglandins (PGs) in mesenteric arteries isolated from sham-operated and totally pancreatectomized dogs. PGE2 and PGF2α produced a similar dose-dependent relaxation of mesenteric arteries from normal and diabetic dogs. On the contrary, NaA and prostacyclin (PGI2) enhanced the resting basal tone of arteries from pancreatectomized animals but depressed it in arteries from intact normal control or from sham-operated groups. Inhibitors of thromboxane A2 (TXA2) biosynthesis abolished in vitro the vasoconstricting effect of NaA and PGI2 in diabetics whereas inhibitors of PGI2 biosynthesis blocked the vasodilating influence of NaA in normal mesenteric vessels. Additionally, antagonists of cycooxygenase activity prevented both the vasoconstricting and the vasodilating actions of NaA in normal and in diabetic arteries, respectively, as well as the PGI2 tone enhancement in vessels from diabetics. In arteries from pancreatectomized animals treated with insulin, PGI2 induced a biphasic (constriction and relaxation) effect of a magnitude between that of effects seen in normal controls or sham-operated and those in untreated diabetic animals. The basal radioconversion of exogenous [1-14C]AA, showed that mesenteric arteries from diabetic dogs generated more TXB2 than did vessels from intact normal control or sham-operated dogs. Moreover, in the presence of exogenous PGI2, the vascular production of TXB2 from AA in the diabetic group was significantly greater than that of preparations not exposed to PGI2. The % conversion of AA into PGI2 (assessed as 6-oxo-PGF1α) was similar in normal controls, in sham-operated and in diabetic vessels. Insulin given in vivo abolished the greater basal conversion of AA into TBX2 by mesenteric arteries from diabetic dogs and significantly attenuated the enhanced prostacyclin-evoked generation of thromboxane. The present results strongly suggest that the abnormal constricting response evoked by NaA and PGI2 in mesenteric arteries from diabetic dogs could be related to the generation of TXA2 by vessel walls. © 1984. 1984 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v103_n3-4_p211_SterinBorda http://hdl.handle.net/20.500.12110/paper_00142999_v103_n3-4_p211_SterinBorda |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
6-oxo-PGF1α Arachidonic acid Arteries Cyclooxygenase Diabetes Insulin Lipoxygenase Mesenteric vessels Pancreatectomized dogs Prostacyclin Prostaglandins Thromboxane Vasoconstriction Vasodilation 5,8,11,14 icosatetraynoic acid acetylsalicylic acid arachidonic acid c 14 haloperidol imidazole indometacin insulin nordihydroguaiaretic acid phentolamine phentolamine mesylate prostacyclin prostaglandin synthase radioisotope thromboxane tranylcypromine unclassified drug animal model article diabetes mellitus dog drug efficacy endocrine system mesenteric artery nonhuman pancreas pancreas resection peripheral vascular system Animals Arachidonic Acid Arachidonic Acids Diabetes Mellitus, Experimental Dinoprost Dinoprostone Dogs Epoprostenol Mesenteric Arteries Muscle Tonus Muscle, Smooth, Vascular Pancreatectomy Prostaglandins Prostaglandins E Prostaglandins F Thromboxanes Vasoconstriction |
| spellingShingle |
6-oxo-PGF1α Arachidonic acid Arteries Cyclooxygenase Diabetes Insulin Lipoxygenase Mesenteric vessels Pancreatectomized dogs Prostacyclin Prostaglandins Thromboxane Vasoconstriction Vasodilation 5,8,11,14 icosatetraynoic acid acetylsalicylic acid arachidonic acid c 14 haloperidol imidazole indometacin insulin nordihydroguaiaretic acid phentolamine phentolamine mesylate prostacyclin prostaglandin synthase radioisotope thromboxane tranylcypromine unclassified drug animal model article diabetes mellitus dog drug efficacy endocrine system mesenteric artery nonhuman pancreas pancreas resection peripheral vascular system Animals Arachidonic Acid Arachidonic Acids Diabetes Mellitus, Experimental Dinoprost Dinoprostone Dogs Epoprostenol Mesenteric Arteries Muscle Tonus Muscle, Smooth, Vascular Pancreatectomy Prostaglandins Prostaglandins E Prostaglandins F Thromboxanes Vasoconstriction Augmented thromboxane generation by mesenteric arteries from pancreatectomized diabetic dogs is coincident with the vascular tone enhancement evoked by Na arachidonate and prostacyclin |
| topic_facet |
6-oxo-PGF1α Arachidonic acid Arteries Cyclooxygenase Diabetes Insulin Lipoxygenase Mesenteric vessels Pancreatectomized dogs Prostacyclin Prostaglandins Thromboxane Vasoconstriction Vasodilation 5,8,11,14 icosatetraynoic acid acetylsalicylic acid arachidonic acid c 14 haloperidol imidazole indometacin insulin nordihydroguaiaretic acid phentolamine phentolamine mesylate prostacyclin prostaglandin synthase radioisotope thromboxane tranylcypromine unclassified drug animal model article diabetes mellitus dog drug efficacy endocrine system mesenteric artery nonhuman pancreas pancreas resection peripheral vascular system Animals Arachidonic Acid Arachidonic Acids Diabetes Mellitus, Experimental Dinoprost Dinoprostone Dogs Epoprostenol Mesenteric Arteries Muscle Tonus Muscle, Smooth, Vascular Pancreatectomy Prostaglandins Prostaglandins E Prostaglandins F Thromboxanes Vasoconstriction |
| description |
We studied the relationships between acrachidonic acid (AA) metabolism and contractile responses to Na arachidonate (NaA) and the prostaglandins (PGs) in mesenteric arteries isolated from sham-operated and totally pancreatectomized dogs. PGE2 and PGF2α produced a similar dose-dependent relaxation of mesenteric arteries from normal and diabetic dogs. On the contrary, NaA and prostacyclin (PGI2) enhanced the resting basal tone of arteries from pancreatectomized animals but depressed it in arteries from intact normal control or from sham-operated groups. Inhibitors of thromboxane A2 (TXA2) biosynthesis abolished in vitro the vasoconstricting effect of NaA and PGI2 in diabetics whereas inhibitors of PGI2 biosynthesis blocked the vasodilating influence of NaA in normal mesenteric vessels. Additionally, antagonists of cycooxygenase activity prevented both the vasoconstricting and the vasodilating actions of NaA in normal and in diabetic arteries, respectively, as well as the PGI2 tone enhancement in vessels from diabetics. In arteries from pancreatectomized animals treated with insulin, PGI2 induced a biphasic (constriction and relaxation) effect of a magnitude between that of effects seen in normal controls or sham-operated and those in untreated diabetic animals. The basal radioconversion of exogenous [1-14C]AA, showed that mesenteric arteries from diabetic dogs generated more TXB2 than did vessels from intact normal control or sham-operated dogs. Moreover, in the presence of exogenous PGI2, the vascular production of TXB2 from AA in the diabetic group was significantly greater than that of preparations not exposed to PGI2. The % conversion of AA into PGI2 (assessed as 6-oxo-PGF1α) was similar in normal controls, in sham-operated and in diabetic vessels. Insulin given in vivo abolished the greater basal conversion of AA into TBX2 by mesenteric arteries from diabetic dogs and significantly attenuated the enhanced prostacyclin-evoked generation of thromboxane. The present results strongly suggest that the abnormal constricting response evoked by NaA and PGI2 in mesenteric arteries from diabetic dogs could be related to the generation of TXA2 by vessel walls. © 1984. |
| title |
Augmented thromboxane generation by mesenteric arteries from pancreatectomized diabetic dogs is coincident with the vascular tone enhancement evoked by Na arachidonate and prostacyclin |
| title_short |
Augmented thromboxane generation by mesenteric arteries from pancreatectomized diabetic dogs is coincident with the vascular tone enhancement evoked by Na arachidonate and prostacyclin |
| title_full |
Augmented thromboxane generation by mesenteric arteries from pancreatectomized diabetic dogs is coincident with the vascular tone enhancement evoked by Na arachidonate and prostacyclin |
| title_fullStr |
Augmented thromboxane generation by mesenteric arteries from pancreatectomized diabetic dogs is coincident with the vascular tone enhancement evoked by Na arachidonate and prostacyclin |
| title_full_unstemmed |
Augmented thromboxane generation by mesenteric arteries from pancreatectomized diabetic dogs is coincident with the vascular tone enhancement evoked by Na arachidonate and prostacyclin |
| title_sort |
augmented thromboxane generation by mesenteric arteries from pancreatectomized diabetic dogs is coincident with the vascular tone enhancement evoked by na arachidonate and prostacyclin |
| publishDate |
1984 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v103_n3-4_p211_SterinBorda http://hdl.handle.net/20.500.12110/paper_00142999_v103_n3-4_p211_SterinBorda |
| _version_ |
1768542969146638336 |