Structural features of the lipopeptidophosphoglycan from Trypanosoma cruzi common with the glycophosphatidylinositol anchors

The lipopeptidophosphoglycan (LPPG) from Trypanosoma cruzi, a major constituent of the plasma membrane of epimastigote forms, has been now extracted with butanol/water from delipidated cells and purified by hydrophobic chromatography. We have found that the LPPG undergoes two reactions, characterist...

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Autores principales: Muchnik de Lederkremer, Rosa María, Ramírez, María Isabel, Casal de Russo, Olga Liliana
Publicado: 1990
Materias:
glycan
lipid
peptide
phospholipase
article
cell membrane
enzyme linked immunosorbent assay
invertebrate
nonhuman
priority journal
protozoon
trypanosoma cruzi
Animal
Ceramides
Chromatography, High Pressure Liquid
Enzyme-Linked Immunosorbent Assay
Glycolipids
Glycosylphosphatidylinositols
Oxidation-Reduction
Peptidoglycan
Phosphatidylinositols
Phospholipase C
Phospholipids
Support, Non-U.S. Gov't
Trypanosoma cruzi
Bacillus thuringiensis
Invertebrata
Protozoa
Trypanosoma
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v192_n2_p337_deLEDERKREMER
http://hdl.handle.net/20.500.12110/paper_00142956_v192_n2_p337_deLEDERKREMER
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00142956_v192_n2_p337_deLEDERKREMER
record_format dspace
spelling paper:paper_00142956_v192_n2_p337_deLEDERKREMER2023-06-08T14:36:41Z Structural features of the lipopeptidophosphoglycan from Trypanosoma cruzi common with the glycophosphatidylinositol anchors Muchnik de Lederkremer, Rosa María Ramírez, María Isabel Casal de Russo, Olga Liliana glycan lipid peptide phospholipase article cell membrane enzyme linked immunosorbent assay invertebrate nonhuman priority journal protozoon trypanosoma cruzi Animal Ceramides Chromatography, High Pressure Liquid Enzyme-Linked Immunosorbent Assay Glycolipids Glycosylphosphatidylinositols Oxidation-Reduction Peptidoglycan Phosphatidylinositols Phospholipase C Phospholipids Support, Non-U.S. Gov't Trypanosoma cruzi Bacillus thuringiensis Invertebrata Protozoa Trypanosoma Trypanosoma cruzi The lipopeptidophosphoglycan (LPPG) from Trypanosoma cruzi, a major constituent of the plasma membrane of epimastigote forms, has been now extracted with butanol/water from delipidated cells and purified by hydrophobic chromatography. We have found that the LPPG undergoes two reactions, characteristic of the glycosylphosphatidylinositol anchors: (a) cleavage of the ceramide by phosphatidylinositol‐specific phospholipase C(PtdIns‐specific phospholipase C) from Bacillus thuringiensis, (b) nitrous acid deamination of the non‐N‐acylated glucosamine. Palmitoylsphinganine, palmitoylsphingosine, lignoceroylsphinganine and, as minor components, the stearoylceramides were identified by gas liquid chromatography/mass spectrometry. The presence of cross reacting determinant (CRD) epitopes in the glycophosphoinositol released by PtdIns‐specific phospholipase C was investigated by direct and inhibition ELISA. A sample of glycophosphoinositol containing 5 μg carbohydrate caused 60% inhibition of the binding of anti‐CRD antibodies raised against the soluble form of variant surface glycoprotein. Copyright © 1990, Wiley Blackwell. All rights reserved Fil:de LEDERKREMER, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:RAMIREZ, M.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:CASAL, O.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1990 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v192_n2_p337_deLEDERKREMER http://hdl.handle.net/20.500.12110/paper_00142956_v192_n2_p337_deLEDERKREMER
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic glycan
lipid
peptide
phospholipase
article
cell membrane
enzyme linked immunosorbent assay
invertebrate
nonhuman
priority journal
protozoon
trypanosoma cruzi
Animal
Ceramides
Chromatography, High Pressure Liquid
Enzyme-Linked Immunosorbent Assay
Glycolipids
Glycosylphosphatidylinositols
Oxidation-Reduction
Peptidoglycan
Phosphatidylinositols
Phospholipase C
Phospholipids
Support, Non-U.S. Gov't
Trypanosoma cruzi
Bacillus thuringiensis
Invertebrata
Protozoa
Trypanosoma
Trypanosoma cruzi
spellingShingle glycan
lipid
peptide
phospholipase
article
cell membrane
enzyme linked immunosorbent assay
invertebrate
nonhuman
priority journal
protozoon
trypanosoma cruzi
Animal
Ceramides
Chromatography, High Pressure Liquid
Enzyme-Linked Immunosorbent Assay
Glycolipids
Glycosylphosphatidylinositols
Oxidation-Reduction
Peptidoglycan
Phosphatidylinositols
Phospholipase C
Phospholipids
Support, Non-U.S. Gov't
Trypanosoma cruzi
Bacillus thuringiensis
Invertebrata
Protozoa
Trypanosoma
Trypanosoma cruzi
Muchnik de Lederkremer, Rosa María
Ramírez, María Isabel
Casal de Russo, Olga Liliana
Structural features of the lipopeptidophosphoglycan from Trypanosoma cruzi common with the glycophosphatidylinositol anchors
topic_facet glycan
lipid
peptide
phospholipase
article
cell membrane
enzyme linked immunosorbent assay
invertebrate
nonhuman
priority journal
protozoon
trypanosoma cruzi
Animal
Ceramides
Chromatography, High Pressure Liquid
Enzyme-Linked Immunosorbent Assay
Glycolipids
Glycosylphosphatidylinositols
Oxidation-Reduction
Peptidoglycan
Phosphatidylinositols
Phospholipase C
Phospholipids
Support, Non-U.S. Gov't
Trypanosoma cruzi
Bacillus thuringiensis
Invertebrata
Protozoa
Trypanosoma
Trypanosoma cruzi
description The lipopeptidophosphoglycan (LPPG) from Trypanosoma cruzi, a major constituent of the plasma membrane of epimastigote forms, has been now extracted with butanol/water from delipidated cells and purified by hydrophobic chromatography. We have found that the LPPG undergoes two reactions, characteristic of the glycosylphosphatidylinositol anchors: (a) cleavage of the ceramide by phosphatidylinositol‐specific phospholipase C(PtdIns‐specific phospholipase C) from Bacillus thuringiensis, (b) nitrous acid deamination of the non‐N‐acylated glucosamine. Palmitoylsphinganine, palmitoylsphingosine, lignoceroylsphinganine and, as minor components, the stearoylceramides were identified by gas liquid chromatography/mass spectrometry. The presence of cross reacting determinant (CRD) epitopes in the glycophosphoinositol released by PtdIns‐specific phospholipase C was investigated by direct and inhibition ELISA. A sample of glycophosphoinositol containing 5 μg carbohydrate caused 60% inhibition of the binding of anti‐CRD antibodies raised against the soluble form of variant surface glycoprotein. Copyright © 1990, Wiley Blackwell. All rights reserved
author Muchnik de Lederkremer, Rosa María
Ramírez, María Isabel
Casal de Russo, Olga Liliana
author_facet Muchnik de Lederkremer, Rosa María
Ramírez, María Isabel
Casal de Russo, Olga Liliana
author_sort Muchnik de Lederkremer, Rosa María
title Structural features of the lipopeptidophosphoglycan from Trypanosoma cruzi common with the glycophosphatidylinositol anchors
title_short Structural features of the lipopeptidophosphoglycan from Trypanosoma cruzi common with the glycophosphatidylinositol anchors
title_full Structural features of the lipopeptidophosphoglycan from Trypanosoma cruzi common with the glycophosphatidylinositol anchors
title_fullStr Structural features of the lipopeptidophosphoglycan from Trypanosoma cruzi common with the glycophosphatidylinositol anchors
title_full_unstemmed Structural features of the lipopeptidophosphoglycan from Trypanosoma cruzi common with the glycophosphatidylinositol anchors
title_sort structural features of the lipopeptidophosphoglycan from trypanosoma cruzi common with the glycophosphatidylinositol anchors
publishDate 1990
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v192_n2_p337_deLEDERKREMER
http://hdl.handle.net/20.500.12110/paper_00142956_v192_n2_p337_deLEDERKREMER
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AT ramirezmariaisabel structuralfeaturesofthelipopeptidophosphoglycanfromtrypanosomacruzicommonwiththeglycophosphatidylinositolanchors
AT casalderussoolgaliliana structuralfeaturesofthelipopeptidophosphoglycanfromtrypanosomacruzicommonwiththeglycophosphatidylinositolanchors
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