Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean
Use of molecular markers such as Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP) and Simple Sequence Repeats (SSR) as descriptors to characterize and differentiate a set of 100 soybean varieties of commercial use in Argentina was taken as a leading case study f...
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paper:paper_00142336_v127_n1_p95_Giancola2023-06-08T14:36:34Z Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean Giancola, Sandra M. Marcucci Poltri, Susana N. Hopp, Horacio Esteban AFLP Glycine max (L.) Merr. RAPD SSR UPGMA Embryophyta Glycine max Use of molecular markers such as Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP) and Simple Sequence Repeats (SSR) as descriptors to characterize and differentiate a set of 100 soybean varieties of commercial use in Argentina was taken as a leading case study for plant variety protection (PVP) purposes. Sixteen morphological traits were recorded to compare pedigree relationships among varieties with information derived from conventional descriptors and molecular markers. Analysis of 109 polymorphic loci confirmed the rather low genetic variability of commercial soybean germplasm. Still, genetic fingerprinting of the 100 varieties could be established. Calculated similarity indexes were dependent on the technique, ranging from 0.262 (SSR), 0.407 (RAPD), 0.400 (AFLP) and 0.574 (morphological traits). Dendrograms generated from morphological data matrix showed low value correlation with kinship coefficient matrix (r = 0.216). Still, they were suitable to identify and differentiate each of the 100 varieties analyzed; which was not possible with RAPD or AFLP markers using comparable numbers of polymorphic loci. SSR data showed the best fit to pedigree information (r = 0.353), while maintaining an association to morphologically based separation. Results suggest that the four techniques describe genetic variability in different and specific ways. A combination of SSR and morphological descriptors show the best compromise of regarding genetic relationships and the needs of clear classification for PVP and may help to establish minimum genetic distances for distinctness within PVP Office definition. Fil:Giancola, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marcucci Poltri, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142336_v127_n1_p95_Giancola http://hdl.handle.net/20.500.12110/paper_00142336_v127_n1_p95_Giancola |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
AFLP Glycine max (L.) Merr. RAPD SSR UPGMA Embryophyta Glycine max |
spellingShingle |
AFLP Glycine max (L.) Merr. RAPD SSR UPGMA Embryophyta Glycine max Giancola, Sandra M. Marcucci Poltri, Susana N. Hopp, Horacio Esteban Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean |
topic_facet |
AFLP Glycine max (L.) Merr. RAPD SSR UPGMA Embryophyta Glycine max |
description |
Use of molecular markers such as Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP) and Simple Sequence Repeats (SSR) as descriptors to characterize and differentiate a set of 100 soybean varieties of commercial use in Argentina was taken as a leading case study for plant variety protection (PVP) purposes. Sixteen morphological traits were recorded to compare pedigree relationships among varieties with information derived from conventional descriptors and molecular markers. Analysis of 109 polymorphic loci confirmed the rather low genetic variability of commercial soybean germplasm. Still, genetic fingerprinting of the 100 varieties could be established. Calculated similarity indexes were dependent on the technique, ranging from 0.262 (SSR), 0.407 (RAPD), 0.400 (AFLP) and 0.574 (morphological traits). Dendrograms generated from morphological data matrix showed low value correlation with kinship coefficient matrix (r = 0.216). Still, they were suitable to identify and differentiate each of the 100 varieties analyzed; which was not possible with RAPD or AFLP markers using comparable numbers of polymorphic loci. SSR data showed the best fit to pedigree information (r = 0.353), while maintaining an association to morphologically based separation. Results suggest that the four techniques describe genetic variability in different and specific ways. A combination of SSR and morphological descriptors show the best compromise of regarding genetic relationships and the needs of clear classification for PVP and may help to establish minimum genetic distances for distinctness within PVP Office definition. |
author |
Giancola, Sandra M. Marcucci Poltri, Susana N. Hopp, Horacio Esteban |
author_facet |
Giancola, Sandra M. Marcucci Poltri, Susana N. Hopp, Horacio Esteban |
author_sort |
Giancola, Sandra M. |
title |
Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean |
title_short |
Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean |
title_full |
Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean |
title_fullStr |
Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean |
title_full_unstemmed |
Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean |
title_sort |
feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean |
publishDate |
2002 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142336_v127_n1_p95_Giancola http://hdl.handle.net/20.500.12110/paper_00142336_v127_n1_p95_Giancola |
work_keys_str_mv |
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