Direct effect of melatonin on Syrian hamster testes: Melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system
Besides the hypothalamus and pituitary, melatonin action at the testicuiar level has been recently suggested. Therefore, we investigated in the Syrian hamster, a well-characterized seasonal breeder, melatonin action on Leydig cells, testicular expression of melatonergic receptors, and possible inter...
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2005
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v146_n3_p1541_Frungieri http://hdl.handle.net/20.500.12110/paper_00137227_v146_n3_p1541_Frungieri |
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paper:paper_00137227_v146_n3_p1541_Frungieri2023-06-08T14:36:11Z Direct effect of melatonin on Syrian hamster testes: Melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system 3(or 17)beta hydroxysteroid dehydrogenase 3alpha hydroxysteroid dehydrogenase androgen androstanediol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin corticotropin releasing factor corticotropin releasing factor receptor 1 cyclic AMP melatonin melatonin 1 receptor melatonin 1A receptor messenger RNA serotonin steroid 5alpha reductase steroidogenic acute regulatory protein testosterone testosterone 17beta dehydrogenase unclassified drug androgen release animal cell animal experiment animal tissue article cell isolation controlled study down regulation hormone action hormone receptor interaction hypophysis hypothalamus Leydig cell male nonhuman nucleotide sequence photoperiodicity priority journal protein expression protein protein interaction protein synthesis serotoninergic system steroidogenesis Syrian hamster testis testosterone metabolism 3-Hydroxysteroid Dehydrogenases Androgens Animals Corticotropin-Releasing Hormone Cricetinae Cyclic AMP DNA Primers Immunoblotting Immunoenzyme Techniques Immunohistochemistry Leydig Cells Light Male Melatonin Mesocricetus Receptors, Melatonin Reverse Transcriptase Polymerase Chain Reaction Testis Testosterone Time Factors Tryptamines Besides the hypothalamus and pituitary, melatonin action at the testicuiar level has been recently suggested. Therefore, we investigated in the Syrian hamster, a well-characterized seasonal breeder, melatonin action on Leydig cells, testicular expression of melatonergic receptors, and possible interactions between melatonin receptors and the previously identified testicular serotoninergic and CRH systems. In isolated Leydig cells from active testes of adult hamsters kept in a long-day (14 h light, 10 h dark) photoperiod and from regressed testes of adult animals exposed to a short-day photoperiod during 16 wk (6 h light, 18 h dark), melatonin significantly reduced human chorionic gonadotropin-stimulated production of cAMP and the main androgens: testosterone and androstane-3α,17β-diol, respectively, and decreased the expression of steroidogenic acute regulatory protein, P450 side chain cleavage, 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase. In Leydig cells exposed to a short-day photoperiod during 16 wk, melatonin stimulated the conversion of testosterone into 5α-reduced androgens by inducing 5α-reductase isoform 1, and controlled androstane-3α,17β-diol production by inhibiting 3α- hydroxysteroid dehydrogenase expression. Melatonin subtype (mella) receptors were detected in Leydig cells. Although the local serotonin system did not mediate melatonin action on androgen production, melatonergic effect on steroidogenesis involved the interaction between mella receptors and the inhibitory CEH system. Moreover, melatonin significantly increased CRH mRNA levels and production in hamster Leydig cells expressing CRH subtype 1 receptors. Our studies indicate that melatonin may act as a local inhibitor of human chorionic gonadotropin-stimulated cAMP and androgen production through mella receptors, down-regulation of steroidogenic acute regulatory protein, and key steroidogenic enzymes expression and its interaction with the local CRH system. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v146_n3_p1541_Frungieri http://hdl.handle.net/20.500.12110/paper_00137227_v146_n3_p1541_Frungieri |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
3(or 17)beta hydroxysteroid dehydrogenase 3alpha hydroxysteroid dehydrogenase androgen androstanediol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin corticotropin releasing factor corticotropin releasing factor receptor 1 cyclic AMP melatonin melatonin 1 receptor melatonin 1A receptor messenger RNA serotonin steroid 5alpha reductase steroidogenic acute regulatory protein testosterone testosterone 17beta dehydrogenase unclassified drug androgen release animal cell animal experiment animal tissue article cell isolation controlled study down regulation hormone action hormone receptor interaction hypophysis hypothalamus Leydig cell male nonhuman nucleotide sequence photoperiodicity priority journal protein expression protein protein interaction protein synthesis serotoninergic system steroidogenesis Syrian hamster testis testosterone metabolism 3-Hydroxysteroid Dehydrogenases Androgens Animals Corticotropin-Releasing Hormone Cricetinae Cyclic AMP DNA Primers Immunoblotting Immunoenzyme Techniques Immunohistochemistry Leydig Cells Light Male Melatonin Mesocricetus Receptors, Melatonin Reverse Transcriptase Polymerase Chain Reaction Testis Testosterone Time Factors Tryptamines |
| spellingShingle |
3(or 17)beta hydroxysteroid dehydrogenase 3alpha hydroxysteroid dehydrogenase androgen androstanediol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin corticotropin releasing factor corticotropin releasing factor receptor 1 cyclic AMP melatonin melatonin 1 receptor melatonin 1A receptor messenger RNA serotonin steroid 5alpha reductase steroidogenic acute regulatory protein testosterone testosterone 17beta dehydrogenase unclassified drug androgen release animal cell animal experiment animal tissue article cell isolation controlled study down regulation hormone action hormone receptor interaction hypophysis hypothalamus Leydig cell male nonhuman nucleotide sequence photoperiodicity priority journal protein expression protein protein interaction protein synthesis serotoninergic system steroidogenesis Syrian hamster testis testosterone metabolism 3-Hydroxysteroid Dehydrogenases Androgens Animals Corticotropin-Releasing Hormone Cricetinae Cyclic AMP DNA Primers Immunoblotting Immunoenzyme Techniques Immunohistochemistry Leydig Cells Light Male Melatonin Mesocricetus Receptors, Melatonin Reverse Transcriptase Polymerase Chain Reaction Testis Testosterone Time Factors Tryptamines Direct effect of melatonin on Syrian hamster testes: Melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system |
| topic_facet |
3(or 17)beta hydroxysteroid dehydrogenase 3alpha hydroxysteroid dehydrogenase androgen androstanediol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin corticotropin releasing factor corticotropin releasing factor receptor 1 cyclic AMP melatonin melatonin 1 receptor melatonin 1A receptor messenger RNA serotonin steroid 5alpha reductase steroidogenic acute regulatory protein testosterone testosterone 17beta dehydrogenase unclassified drug androgen release animal cell animal experiment animal tissue article cell isolation controlled study down regulation hormone action hormone receptor interaction hypophysis hypothalamus Leydig cell male nonhuman nucleotide sequence photoperiodicity priority journal protein expression protein protein interaction protein synthesis serotoninergic system steroidogenesis Syrian hamster testis testosterone metabolism 3-Hydroxysteroid Dehydrogenases Androgens Animals Corticotropin-Releasing Hormone Cricetinae Cyclic AMP DNA Primers Immunoblotting Immunoenzyme Techniques Immunohistochemistry Leydig Cells Light Male Melatonin Mesocricetus Receptors, Melatonin Reverse Transcriptase Polymerase Chain Reaction Testis Testosterone Time Factors Tryptamines |
| description |
Besides the hypothalamus and pituitary, melatonin action at the testicuiar level has been recently suggested. Therefore, we investigated in the Syrian hamster, a well-characterized seasonal breeder, melatonin action on Leydig cells, testicular expression of melatonergic receptors, and possible interactions between melatonin receptors and the previously identified testicular serotoninergic and CRH systems. In isolated Leydig cells from active testes of adult hamsters kept in a long-day (14 h light, 10 h dark) photoperiod and from regressed testes of adult animals exposed to a short-day photoperiod during 16 wk (6 h light, 18 h dark), melatonin significantly reduced human chorionic gonadotropin-stimulated production of cAMP and the main androgens: testosterone and androstane-3α,17β-diol, respectively, and decreased the expression of steroidogenic acute regulatory protein, P450 side chain cleavage, 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase. In Leydig cells exposed to a short-day photoperiod during 16 wk, melatonin stimulated the conversion of testosterone into 5α-reduced androgens by inducing 5α-reductase isoform 1, and controlled androstane-3α,17β-diol production by inhibiting 3α- hydroxysteroid dehydrogenase expression. Melatonin subtype (mella) receptors were detected in Leydig cells. Although the local serotonin system did not mediate melatonin action on androgen production, melatonergic effect on steroidogenesis involved the interaction between mella receptors and the inhibitory CEH system. Moreover, melatonin significantly increased CRH mRNA levels and production in hamster Leydig cells expressing CRH subtype 1 receptors. Our studies indicate that melatonin may act as a local inhibitor of human chorionic gonadotropin-stimulated cAMP and androgen production through mella receptors, down-regulation of steroidogenic acute regulatory protein, and key steroidogenic enzymes expression and its interaction with the local CRH system. |
| title |
Direct effect of melatonin on Syrian hamster testes: Melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system |
| title_short |
Direct effect of melatonin on Syrian hamster testes: Melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system |
| title_full |
Direct effect of melatonin on Syrian hamster testes: Melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system |
| title_fullStr |
Direct effect of melatonin on Syrian hamster testes: Melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system |
| title_full_unstemmed |
Direct effect of melatonin on Syrian hamster testes: Melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system |
| title_sort |
direct effect of melatonin on syrian hamster testes: melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system |
| publishDate |
2005 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v146_n3_p1541_Frungieri http://hdl.handle.net/20.500.12110/paper_00137227_v146_n3_p1541_Frungieri |
| _version_ |
1768545767790739456 |