Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway

Bacterial lipopolysaccharide (LPS) activates the immune system and induces increases in peripheral cytokines, which, in turn, affect the endocrine system. In particular, LPS-induced cytokines stimulate the hypothalamic-pituitary-adrenal axis to increase levels of antiinflammatory-acting glucocortico...

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Autores principales:	Carbia Nagashima, Alberto Nicolás, Páez Pereda, Marcelo
Publicado:	2000
Materias:	4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole antibody CD14 antigen cycloheximide dexamethasone immunoglobulin enhancer binding protein interleukin 6 lipopolysaccharide lipopolysaccharide binding protein mitogen activated protein kinase toll like receptor 4 animal cell antigen expression article cell line cell stimulation complex formation controlled study cytokine production cytokine release hypophysis cell male mouse nonhuman priority journal protein metabolism protein phosphorylation signal transduction
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v141_n12_p4457_Lohrer http://hdl.handle.net/20.500.12110/paper_00137227_v141_n12_p4457_Lohrer
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00137227_v141_n12_p4457_Lohrer
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spelling	paper:paper_00137227_v141_n12_p4457_Lohrer2023-06-08T14:36:09Z Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway Carbia Nagashima, Alberto Nicolás Páez Pereda, Marcelo 4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole antibody CD14 antigen cycloheximide dexamethasone immunoglobulin enhancer binding protein interleukin 6 lipopolysaccharide lipopolysaccharide binding protein mitogen activated protein kinase toll like receptor 4 animal cell antigen expression article cell line cell stimulation complex formation controlled study cytokine production cytokine release hypophysis cell male mouse nonhuman priority journal protein metabolism protein phosphorylation signal transduction Bacterial lipopolysaccharide (LPS) activates the immune system and induces increases in peripheral cytokines, which, in turn, affect the endocrine system. In particular, LPS-induced cytokines stimulate the hypothalamic-pituitary-adrenal axis to increase levels of antiinflammatory-acting glucocorticoids. In the present work, we show that LPS directly stimulates interleukin (IL)-6 release by mouse pituitary folliculostellate (FS) TtT/GF tumor cells and FS cells of mouse pituitary cell cultures. The stimulatory effect of LPS was strongly enhanced in the presence of serum, suggesting that LPS is only fully active as a complex with LPS-binding protein (LBP). Both TtT/GF cells and mouse pituitaries expressed CD14, which binds the LPS/LBP complex, and Toll-like receptor type 4, which induces LPS signals. LPS increased phospoinositol turnover in TtT/GF cells and induced phosphorylation of p38α mitogen-activated protein kinase and the inhibitor (IκB) of nuclear factor-κ B. Nuclear factor-κ B was activated by LPS in TtT/GF cells. Functional studies demonstrated that My4 (an antibody blocking the interaction between LPS/LBP and CD14), SB203580, (a specific inhibitor of p38α mitogen-activated protein kinase phosphorylation), dexamethasone, and the messenger RNA translation inhibitor cycloheximide all inhibited LPS-induced IL-6 production by TtT/GF cells and mouse pituitary FS cells. LPS-induced intrapituitary IL-6 may modulate the function of anterior pituitary cells during bacterial infection/inflammation. Fil:Carbia Nagashima, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paez Pereda, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2000 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v141_n12_p4457_Lohrer http://hdl.handle.net/20.500.12110/paper_00137227_v141_n12_p4457_Lohrer
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole antibody CD14 antigen cycloheximide dexamethasone immunoglobulin enhancer binding protein interleukin 6 lipopolysaccharide lipopolysaccharide binding protein mitogen activated protein kinase toll like receptor 4 animal cell antigen expression article cell line cell stimulation complex formation controlled study cytokine production cytokine release hypophysis cell male mouse nonhuman priority journal protein metabolism protein phosphorylation signal transduction
spellingShingle	4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole antibody CD14 antigen cycloheximide dexamethasone immunoglobulin enhancer binding protein interleukin 6 lipopolysaccharide lipopolysaccharide binding protein mitogen activated protein kinase toll like receptor 4 animal cell antigen expression article cell line cell stimulation complex formation controlled study cytokine production cytokine release hypophysis cell male mouse nonhuman priority journal protein metabolism protein phosphorylation signal transduction Carbia Nagashima, Alberto Nicolás Páez Pereda, Marcelo Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway
topic_facet	4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole antibody CD14 antigen cycloheximide dexamethasone immunoglobulin enhancer binding protein interleukin 6 lipopolysaccharide lipopolysaccharide binding protein mitogen activated protein kinase toll like receptor 4 animal cell antigen expression article cell line cell stimulation complex formation controlled study cytokine production cytokine release hypophysis cell male mouse nonhuman priority journal protein metabolism protein phosphorylation signal transduction
description	Bacterial lipopolysaccharide (LPS) activates the immune system and induces increases in peripheral cytokines, which, in turn, affect the endocrine system. In particular, LPS-induced cytokines stimulate the hypothalamic-pituitary-adrenal axis to increase levels of antiinflammatory-acting glucocorticoids. In the present work, we show that LPS directly stimulates interleukin (IL)-6 release by mouse pituitary folliculostellate (FS) TtT/GF tumor cells and FS cells of mouse pituitary cell cultures. The stimulatory effect of LPS was strongly enhanced in the presence of serum, suggesting that LPS is only fully active as a complex with LPS-binding protein (LBP). Both TtT/GF cells and mouse pituitaries expressed CD14, which binds the LPS/LBP complex, and Toll-like receptor type 4, which induces LPS signals. LPS increased phospoinositol turnover in TtT/GF cells and induced phosphorylation of p38α mitogen-activated protein kinase and the inhibitor (IκB) of nuclear factor-κ B. Nuclear factor-κ B was activated by LPS in TtT/GF cells. Functional studies demonstrated that My4 (an antibody blocking the interaction between LPS/LBP and CD14), SB203580, (a specific inhibitor of p38α mitogen-activated protein kinase phosphorylation), dexamethasone, and the messenger RNA translation inhibitor cycloheximide all inhibited LPS-induced IL-6 production by TtT/GF cells and mouse pituitary FS cells. LPS-induced intrapituitary IL-6 may modulate the function of anterior pituitary cells during bacterial infection/inflammation.
author	Carbia Nagashima, Alberto Nicolás Páez Pereda, Marcelo
author_facet	Carbia Nagashima, Alberto Nicolás Páez Pereda, Marcelo
author_sort	Carbia Nagashima, Alberto Nicolás
title	Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway
title_short	Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway
title_full	Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway
title_fullStr	Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway
title_full_unstemmed	Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway
title_sort	lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κb pathway
publishDate	2000
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v141_n12_p4457_Lohrer http://hdl.handle.net/20.500.12110/paper_00137227_v141_n12_p4457_Lohrer
work_keys_str_mv	AT carbianagashimaalbertonicolas lipopolysaccharidedirectlystimulatestheintrapituitaryinterleukin6productionbyfolliculostellatecellsviaspecificreceptorsandthep38amitogenactivatedproteinkinasenuclearfactorkbpathway AT paezperedamarcelo lipopolysaccharidedirectlystimulatestheintrapituitaryinterleukin6productionbyfolliculostellatecellsviaspecificreceptorsandthep38amitogenactivatedproteinkinasenuclearfactorkbpathway
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Lipopolysaccharide directly stimulates the intrapituitary interleukin-6 production by folliculostellate cells via specific receptors and the p38α mitogen-activated protein kinase/nuclear factor-κB pathway

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