Comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: Response to lh-releasing hormone

The effect of streptozotocin-induced diabetes on circulating levels of immunoactive LH (I-LH) and bioactive LH (B-LH) was investigated. LH was measured in adult ovariectomized (OVX) rats before and after acute LHRH administration, with or without estradiol benzoate (Eb) treatment (10 μg, 48 and 24...

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Detalles Bibliográficos
Autores principales: Tesone, Marta, Ladenheim, Ruth Graciela, Charreau, Eduardo Hernán
Publicado: 1986
Materias:
estradiol
estradiol benzoate
gonadorelin
isophane insulin
luteinizing hormone
streptozocin
animal experiment
animal model
central nervous system
drug activity
drug efficacy
endocrine system
female genital system
immunity
intravenous drug administration
methodology
nonhuman
ovariectomy
priority journal
rat
streptozocin diabetes
subcutaneous drug administration
Animals
Biological Assay
Diabetes Mellitus, Experimental
Estradiol
Female
Gonadotropin-Releasing Hormone
Immunoassay
Insulin
Luteinizing Hormone
Ovariectomy
Pituitary Gland
Rats
Streptozocin
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v119_n6_p2412_Tesone
http://hdl.handle.net/20.500.12110/paper_00137227_v119_n6_p2412_Tesone
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00137227_v119_n6_p2412_Tesone
record_format dspace
spelling paper:paper_00137227_v119_n6_p2412_Tesone2023-06-08T14:36:05Z Comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: Response to lh-releasing hormone Tesone, Marta Ladenheim, Ruth Graciela Charreau, Eduardo Hernán estradiol estradiol benzoate gonadorelin isophane insulin luteinizing hormone streptozocin animal experiment animal model central nervous system drug activity drug efficacy endocrine system female genital system immunity intravenous drug administration methodology nonhuman ovariectomy priority journal rat streptozocin diabetes subcutaneous drug administration Animals Biological Assay Diabetes Mellitus, Experimental Estradiol Female Gonadotropin-Releasing Hormone Immunoassay Insulin Luteinizing Hormone Ovariectomy Pituitary Gland Rats Streptozocin The effect of streptozotocin-induced diabetes on circulating levels of immunoactive LH (I-LH) and bioactive LH (B-LH) was investigated. LH was measured in adult ovariectomized (OVX) rats before and after acute LHRH administration, with or without estradiol benzoate (Eb) treatment (10 μg, 48 and 24 h before experiments). I-LH and B-LH were measured in the same samples by RIA and the rat interstitial cell testosterone assay, respectively. OVX diabetic animals showed a significant reduction in both I-LH (63%) and B-LH (73%). Treatment with Eb induced a decrease in basal I-LH and B-LH levels in all experimental groups (50%). These values were normalized after insulin therapy. No alterations in the pituitary responsiveness to LHRH were detected when I-LH levels were determined. However, B-LH levels assayed after LHRH stimulation were significantly decreased in diabetic animals. Insulin treatment was unable to restore this response. The effect of Eb treatment on these parameters was also tested. In these conditions LHRH injections induced similar increases in serum I-LH and B-LH in both diabetic and control rats. These results indicate that, in diabetic OVX rats, basal and LHRH-induced LH has a reduced bioactivity, but this reduction is reversed by Eb treatment. This might indicate that the major defect lies in the ovary rather than at the pituitary level, supporting the notion of an important role of the steroid milieu on the B-LH modulation. © 1986 by The Endocrine Society. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ladenheim, R.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1986 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v119_n6_p2412_Tesone http://hdl.handle.net/20.500.12110/paper_00137227_v119_n6_p2412_Tesone
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic estradiol
estradiol benzoate
gonadorelin
isophane insulin
luteinizing hormone
streptozocin
animal experiment
animal model
central nervous system
drug activity
drug efficacy
endocrine system
female genital system
immunity
intravenous drug administration
methodology
nonhuman
ovariectomy
priority journal
rat
streptozocin diabetes
subcutaneous drug administration
Animals
Biological Assay
Diabetes Mellitus, Experimental
Estradiol
Female
Gonadotropin-Releasing Hormone
Immunoassay
Insulin
Luteinizing Hormone
Ovariectomy
Pituitary Gland
Rats
Streptozocin
spellingShingle estradiol
estradiol benzoate
gonadorelin
isophane insulin
luteinizing hormone
streptozocin
animal experiment
animal model
central nervous system
drug activity
drug efficacy
endocrine system
female genital system
immunity
intravenous drug administration
methodology
nonhuman
ovariectomy
priority journal
rat
streptozocin diabetes
subcutaneous drug administration
Animals
Biological Assay
Diabetes Mellitus, Experimental
Estradiol
Female
Gonadotropin-Releasing Hormone
Immunoassay
Insulin
Luteinizing Hormone
Ovariectomy
Pituitary Gland
Rats
Streptozocin
Tesone, Marta
Ladenheim, Ruth Graciela
Charreau, Eduardo Hernán
Comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: Response to lh-releasing hormone
topic_facet estradiol
estradiol benzoate
gonadorelin
isophane insulin
luteinizing hormone
streptozocin
animal experiment
animal model
central nervous system
drug activity
drug efficacy
endocrine system
female genital system
immunity
intravenous drug administration
methodology
nonhuman
ovariectomy
priority journal
rat
streptozocin diabetes
subcutaneous drug administration
Animals
Biological Assay
Diabetes Mellitus, Experimental
Estradiol
Female
Gonadotropin-Releasing Hormone
Immunoassay
Insulin
Luteinizing Hormone
Ovariectomy
Pituitary Gland
Rats
Streptozocin
description The effect of streptozotocin-induced diabetes on circulating levels of immunoactive LH (I-LH) and bioactive LH (B-LH) was investigated. LH was measured in adult ovariectomized (OVX) rats before and after acute LHRH administration, with or without estradiol benzoate (Eb) treatment (10 μg, 48 and 24 h before experiments). I-LH and B-LH were measured in the same samples by RIA and the rat interstitial cell testosterone assay, respectively. OVX diabetic animals showed a significant reduction in both I-LH (63%) and B-LH (73%). Treatment with Eb induced a decrease in basal I-LH and B-LH levels in all experimental groups (50%). These values were normalized after insulin therapy. No alterations in the pituitary responsiveness to LHRH were detected when I-LH levels were determined. However, B-LH levels assayed after LHRH stimulation were significantly decreased in diabetic animals. Insulin treatment was unable to restore this response. The effect of Eb treatment on these parameters was also tested. In these conditions LHRH injections induced similar increases in serum I-LH and B-LH in both diabetic and control rats. These results indicate that, in diabetic OVX rats, basal and LHRH-induced LH has a reduced bioactivity, but this reduction is reversed by Eb treatment. This might indicate that the major defect lies in the ovary rather than at the pituitary level, supporting the notion of an important role of the steroid milieu on the B-LH modulation. © 1986 by The Endocrine Society.
author Tesone, Marta
Ladenheim, Ruth Graciela
Charreau, Eduardo Hernán
author_facet Tesone, Marta
Ladenheim, Ruth Graciela
Charreau, Eduardo Hernán
author_sort Tesone, Marta
title Comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: Response to lh-releasing hormone
title_short Comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: Response to lh-releasing hormone
title_full Comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: Response to lh-releasing hormone
title_fullStr Comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: Response to lh-releasing hormone
title_full_unstemmed Comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: Response to lh-releasing hormone
title_sort comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: response to lh-releasing hormone
publishDate 1986
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v119_n6_p2412_Tesone
http://hdl.handle.net/20.500.12110/paper_00137227_v119_n6_p2412_Tesone
work_keys_str_mv AT tesonemarta comparisonbetweenbioactiveandimmunoactiveluteinizinghormonelhinovariectomizedstreptozotocininduceddiabeticratsresponsetolhreleasinghormone
AT ladenheimruthgraciela comparisonbetweenbioactiveandimmunoactiveluteinizinghormonelhinovariectomizedstreptozotocininduceddiabeticratsresponsetolhreleasinghormone
AT charreaueduardohernan comparisonbetweenbioactiveandimmunoactiveluteinizinghormonelhinovariectomizedstreptozotocininduceddiabeticratsresponsetolhreleasinghormone
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