Androgens control androgen-binding sites in rat epididymis
Previous results suggested that the number of androgen-binding sites in rat epididymis was controlled by androgens. Using an exchange technique for receptor determination, we now report that cytoplasmic receptor number decreased to about half the control value after 4 days of castration and reached...
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1983
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v113_n3_p1025_Tezon http://hdl.handle.net/20.500.12110/paper_00137227_v113_n3_p1025_Tezon |
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paper:paper_00137227_v113_n3_p1025_Tezon2023-06-08T14:36:03Z Androgens control androgen-binding sites in rat epididymis androgen cell nucleus receptor cytosol receptor radioisotope testosterone propionate animal cell castration drug efficacy drug receptor binding endocrine system epididymis male genital system methyltrienolone h 3 nonhuman pharmacokinetics rat thymidine h 3 Androgens Animal Castration Cell Nucleus Cytoplasm Epididymis Estrenes Male Metribolone Peptide Hydrolases Rats Rats, Inbred Strains Receptors, Androgen Receptors, Steroid Support, Non-U.S. Gov't Testosterone Previous results suggested that the number of androgen-binding sites in rat epididymis was controlled by androgens. Using an exchange technique for receptor determination, we now report that cytoplasmic receptor number decreased to about half the control value after 4 days of castration and reached its lowest level (30% of the control value) after 12 days of castration. The Kd for [3H]methyltrienolone for cytoplasmic receptor varied from 2.8 × 10−9 M in controls to 0.6 × 10−9 M (P < 0.001) after 6 or more days of castration. Nuclear binding sites were reduced to 17% of the control value from the second day of castration on, but no change in the affinity for methyltrienolone was noted. The administration of testosterone propionate (400 μg/day) to rats castrated for 20 days significantly increased the number of nuclear and cytoplasmic binding sites from the second and third days of treatment, respectively. Labeled thymidine incorporation into DNA rose significantly after a 4-day latency period. The proportion of total binding sites capable of translocation into the nucleus was elevated during the early phase (2–4 days) of androgen treatment. These results also suggest the heterogeneity of the cytoplasmic binding site population. Control epididymides were composed of 61.5% receptor-containing epithelial cells, and this proportion was significantly reduced to 53.2% after 20 days of castration. Androgen administration elevated this percentage after an 8-day latency period. Proteolytic activity in cytosol was increased over control values after castration (for 20 days) and until the fourth day after the onset of androgen treatment. However, this activity does not seem to be an important factor in the decrease in binding sites caused by orchidectomy. © 1983 by The Endocrine Society. 1983 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v113_n3_p1025_Tezon http://hdl.handle.net/20.500.12110/paper_00137227_v113_n3_p1025_Tezon |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
androgen cell nucleus receptor cytosol receptor radioisotope testosterone propionate animal cell castration drug efficacy drug receptor binding endocrine system epididymis male genital system methyltrienolone h 3 nonhuman pharmacokinetics rat thymidine h 3 Androgens Animal Castration Cell Nucleus Cytoplasm Epididymis Estrenes Male Metribolone Peptide Hydrolases Rats Rats, Inbred Strains Receptors, Androgen Receptors, Steroid Support, Non-U.S. Gov't Testosterone |
| spellingShingle |
androgen cell nucleus receptor cytosol receptor radioisotope testosterone propionate animal cell castration drug efficacy drug receptor binding endocrine system epididymis male genital system methyltrienolone h 3 nonhuman pharmacokinetics rat thymidine h 3 Androgens Animal Castration Cell Nucleus Cytoplasm Epididymis Estrenes Male Metribolone Peptide Hydrolases Rats Rats, Inbred Strains Receptors, Androgen Receptors, Steroid Support, Non-U.S. Gov't Testosterone Androgens control androgen-binding sites in rat epididymis |
| topic_facet |
androgen cell nucleus receptor cytosol receptor radioisotope testosterone propionate animal cell castration drug efficacy drug receptor binding endocrine system epididymis male genital system methyltrienolone h 3 nonhuman pharmacokinetics rat thymidine h 3 Androgens Animal Castration Cell Nucleus Cytoplasm Epididymis Estrenes Male Metribolone Peptide Hydrolases Rats Rats, Inbred Strains Receptors, Androgen Receptors, Steroid Support, Non-U.S. Gov't Testosterone |
| description |
Previous results suggested that the number of androgen-binding sites in rat epididymis was controlled by androgens. Using an exchange technique for receptor determination, we now report that cytoplasmic receptor number decreased to about half the control value after 4 days of castration and reached its lowest level (30% of the control value) after 12 days of castration. The Kd for [3H]methyltrienolone for cytoplasmic receptor varied from 2.8 × 10−9 M in controls to 0.6 × 10−9 M (P < 0.001) after 6 or more days of castration. Nuclear binding sites were reduced to 17% of the control value from the second day of castration on, but no change in the affinity for methyltrienolone was noted. The administration of testosterone propionate (400 μg/day) to rats castrated for 20 days significantly increased the number of nuclear and cytoplasmic binding sites from the second and third days of treatment, respectively. Labeled thymidine incorporation into DNA rose significantly after a 4-day latency period. The proportion of total binding sites capable of translocation into the nucleus was elevated during the early phase (2–4 days) of androgen treatment. These results also suggest the heterogeneity of the cytoplasmic binding site population. Control epididymides were composed of 61.5% receptor-containing epithelial cells, and this proportion was significantly reduced to 53.2% after 20 days of castration. Androgen administration elevated this percentage after an 8-day latency period. Proteolytic activity in cytosol was increased over control values after castration (for 20 days) and until the fourth day after the onset of androgen treatment. However, this activity does not seem to be an important factor in the decrease in binding sites caused by orchidectomy. © 1983 by The Endocrine Society. |
| title |
Androgens control androgen-binding sites in rat epididymis |
| title_short |
Androgens control androgen-binding sites in rat epididymis |
| title_full |
Androgens control androgen-binding sites in rat epididymis |
| title_fullStr |
Androgens control androgen-binding sites in rat epididymis |
| title_full_unstemmed |
Androgens control androgen-binding sites in rat epididymis |
| title_sort |
androgens control androgen-binding sites in rat epididymis |
| publishDate |
1983 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v113_n3_p1025_Tezon http://hdl.handle.net/20.500.12110/paper_00137227_v113_n3_p1025_Tezon |
| _version_ |
1768544348931096576 |