Oxygen cathode based on a layer-by-layer self-assembled laccase and osmium redox mediator
Trametes trogii laccase has been studied as biocatalyst for the oxygen electro-reduction in three different systems: (i) soluble laccase was studied in solution; (ii) an enzyme monolayer was tethered to a gold surface by dithiobis N-succinimidyl propionate (DTSP), with a soluble osmium pyridine-bipy...
Guardado en:
| Autores principales: | , , |
|---|---|
| Publicado: |
2009
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00134686_v54_n7_p1970_Szamocki http://hdl.handle.net/20.500.12110/paper_00134686_v54_n7_p1970_Szamocki |
| Aporte de: |
| id |
paper:paper_00134686_v54_n7_p1970_Szamocki |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_00134686_v54_n7_p1970_Szamocki2023-06-08T14:35:58Z Oxygen cathode based on a layer-by-layer self-assembled laccase and osmium redox mediator Flexer, Victoria Levin, Laura Noemi Calvo, Ernesto Julio Electrocatalysis Laccase Layer-by-layer self-assembly Oxygen reducing cathode Redox polymers Adsorption Aviation Catalysis Chlorine compounds Electrocatalysis Electrochemistry Electrolysis Electrolytic reduction Enzymes Gold compounds Methanol Nonmetals Osmium Oxide minerals Oxygen Quartz Quartz crystal microbalances Self assembly Bipyridine Catalytic reductions Covalently bounds Direct electron transfers Electro reductions Fully integrated Gold surfaces Laccase Layer by layers Layer-by-layer self-assembly Linear-scan voltammetries Negative charges Osmium complexes Oxygen cathodes Oxygen partial pressures Oxygen reducing cathode Oxygen reductions Poly(allylamine) Polyanion Polycation Quartz crystals Redox mediators Redox polymers Self-assembled Gas adsorption Trametes trogii laccase has been studied as biocatalyst for the oxygen electro-reduction in three different systems: (i) soluble laccase was studied in solution; (ii) an enzyme monolayer was tethered to a gold surface by dithiobis N-succinimidyl propionate (DTSP), with a soluble osmium pyridine-bipyridine redox mediator in both cases. The third case (iii) consisted in the sequential immobilization of laccase and the osmium complex derivatized poly(allylamine) self-assembled layer-by-layer (LbL) on mercaptopropane sulfonate modified gold to produce an all integrated and wired enzymatic oxygen cathode. The polycation was the same osmium complex covalently bound to poly-(ally-lamine) backbone (PAH-Os), the polyanion was the enzyme adsorbed from a solution of a suitable pH so that the protein carries a net negative charge. The adsorption of laccase was studied by monitoring the mass uptake with a quartz crystal microbalance and the oxygen reduction electrocatalysis was studied by linear scan voltammetry. While for the three cases, oxygen electrocatalysis mediated by the osmium complex was observed, for tethered laccase direct electron transfer in the absence of redox mediator was also apparent but no electrocatalysis for the oxygen reduction was recorded in the absence of mediator in solution. For the fully integrated LbL self-assembled laccase and redox mediator (case iii) a catalytic reduction of oxygen could be recorded at different oxygen partial pressures and different electrolyte pH. The tolerance of the reaction to methanol and chloride was also investigated. © 2008 Elsevier Ltd. All rights reserved. Fil:Flexer, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00134686_v54_n7_p1970_Szamocki http://hdl.handle.net/20.500.12110/paper_00134686_v54_n7_p1970_Szamocki |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Electrocatalysis Laccase Layer-by-layer self-assembly Oxygen reducing cathode Redox polymers Adsorption Aviation Catalysis Chlorine compounds Electrocatalysis Electrochemistry Electrolysis Electrolytic reduction Enzymes Gold compounds Methanol Nonmetals Osmium Oxide minerals Oxygen Quartz Quartz crystal microbalances Self assembly Bipyridine Catalytic reductions Covalently bounds Direct electron transfers Electro reductions Fully integrated Gold surfaces Laccase Layer by layers Layer-by-layer self-assembly Linear-scan voltammetries Negative charges Osmium complexes Oxygen cathodes Oxygen partial pressures Oxygen reducing cathode Oxygen reductions Poly(allylamine) Polyanion Polycation Quartz crystals Redox mediators Redox polymers Self-assembled Gas adsorption |
| spellingShingle |
Electrocatalysis Laccase Layer-by-layer self-assembly Oxygen reducing cathode Redox polymers Adsorption Aviation Catalysis Chlorine compounds Electrocatalysis Electrochemistry Electrolysis Electrolytic reduction Enzymes Gold compounds Methanol Nonmetals Osmium Oxide minerals Oxygen Quartz Quartz crystal microbalances Self assembly Bipyridine Catalytic reductions Covalently bounds Direct electron transfers Electro reductions Fully integrated Gold surfaces Laccase Layer by layers Layer-by-layer self-assembly Linear-scan voltammetries Negative charges Osmium complexes Oxygen cathodes Oxygen partial pressures Oxygen reducing cathode Oxygen reductions Poly(allylamine) Polyanion Polycation Quartz crystals Redox mediators Redox polymers Self-assembled Gas adsorption Flexer, Victoria Levin, Laura Noemi Calvo, Ernesto Julio Oxygen cathode based on a layer-by-layer self-assembled laccase and osmium redox mediator |
| topic_facet |
Electrocatalysis Laccase Layer-by-layer self-assembly Oxygen reducing cathode Redox polymers Adsorption Aviation Catalysis Chlorine compounds Electrocatalysis Electrochemistry Electrolysis Electrolytic reduction Enzymes Gold compounds Methanol Nonmetals Osmium Oxide minerals Oxygen Quartz Quartz crystal microbalances Self assembly Bipyridine Catalytic reductions Covalently bounds Direct electron transfers Electro reductions Fully integrated Gold surfaces Laccase Layer by layers Layer-by-layer self-assembly Linear-scan voltammetries Negative charges Osmium complexes Oxygen cathodes Oxygen partial pressures Oxygen reducing cathode Oxygen reductions Poly(allylamine) Polyanion Polycation Quartz crystals Redox mediators Redox polymers Self-assembled Gas adsorption |
| description |
Trametes trogii laccase has been studied as biocatalyst for the oxygen electro-reduction in three different systems: (i) soluble laccase was studied in solution; (ii) an enzyme monolayer was tethered to a gold surface by dithiobis N-succinimidyl propionate (DTSP), with a soluble osmium pyridine-bipyridine redox mediator in both cases. The third case (iii) consisted in the sequential immobilization of laccase and the osmium complex derivatized poly(allylamine) self-assembled layer-by-layer (LbL) on mercaptopropane sulfonate modified gold to produce an all integrated and wired enzymatic oxygen cathode. The polycation was the same osmium complex covalently bound to poly-(ally-lamine) backbone (PAH-Os), the polyanion was the enzyme adsorbed from a solution of a suitable pH so that the protein carries a net negative charge. The adsorption of laccase was studied by monitoring the mass uptake with a quartz crystal microbalance and the oxygen reduction electrocatalysis was studied by linear scan voltammetry. While for the three cases, oxygen electrocatalysis mediated by the osmium complex was observed, for tethered laccase direct electron transfer in the absence of redox mediator was also apparent but no electrocatalysis for the oxygen reduction was recorded in the absence of mediator in solution. For the fully integrated LbL self-assembled laccase and redox mediator (case iii) a catalytic reduction of oxygen could be recorded at different oxygen partial pressures and different electrolyte pH. The tolerance of the reaction to methanol and chloride was also investigated. © 2008 Elsevier Ltd. All rights reserved. |
| author |
Flexer, Victoria Levin, Laura Noemi Calvo, Ernesto Julio |
| author_facet |
Flexer, Victoria Levin, Laura Noemi Calvo, Ernesto Julio |
| author_sort |
Flexer, Victoria |
| title |
Oxygen cathode based on a layer-by-layer self-assembled laccase and osmium redox mediator |
| title_short |
Oxygen cathode based on a layer-by-layer self-assembled laccase and osmium redox mediator |
| title_full |
Oxygen cathode based on a layer-by-layer self-assembled laccase and osmium redox mediator |
| title_fullStr |
Oxygen cathode based on a layer-by-layer self-assembled laccase and osmium redox mediator |
| title_full_unstemmed |
Oxygen cathode based on a layer-by-layer self-assembled laccase and osmium redox mediator |
| title_sort |
oxygen cathode based on a layer-by-layer self-assembled laccase and osmium redox mediator |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00134686_v54_n7_p1970_Szamocki http://hdl.handle.net/20.500.12110/paper_00134686_v54_n7_p1970_Szamocki |
| work_keys_str_mv |
AT flexervictoria oxygencathodebasedonalayerbylayerselfassembledlaccaseandosmiumredoxmediator AT levinlauranoemi oxygencathodebasedonalayerbylayerselfassembledlaccaseandosmiumredoxmediator AT calvoernestojulio oxygencathodebasedonalayerbylayerselfassembledlaccaseandosmiumredoxmediator |
| _version_ |
1768543734029352960 |