Exchange protein activated by cyclic AMP is involved in the regulation of adipogenic genes during 3T3-L1 fibroblasts differentiation

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Adipogenesis is stimulated in 3T3-L1 fibroblasts by a combination of insulin, dexamethasone and isobutylmethylxanthine, IBMX, (I+D+M). Two transcription factors are important for the acquisition of the adipocyte phenotype, C/EBP beta (CCAT enhancer-binding protein beta) and PPAR gamma (peroxisome pr...

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Autores principales: Gabrielli, Matías, Martini, Claudia Noemí, Raiger-Iustman, Laura Judith, Romero, Damián Gastón, Vila, María del Carmen
Publicado: 2014
Materias:
3T3-L1 cells
Adipogenesis
CCAT enhancer-binding protein beta
Cyclic AMP
Peroxisome proliferator-activated receptor gamma
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00121592_v_n_p_Gabrielli
http://hdl.handle.net/20.500.12110/paper_00121592_v_n_p_Gabrielli
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00121592_v_n_p_Gabrielli
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spelling paper:paper_00121592_v_n_p_Gabrielli2023-06-08T14:35:19Z Exchange protein activated by cyclic AMP is involved in the regulation of adipogenic genes during 3T3-L1 fibroblasts differentiation Gabrielli, Matías Martini, Claudia Noemí Raiger-Iustman, Laura Judith Romero, Damián Gastón Vila, María del Carmen 3T3-L1 cells Adipogenesis CCAT enhancer-binding protein beta Cyclic AMP Peroxisome proliferator-activated receptor gamma Adipogenesis is stimulated in 3T3-L1 fibroblasts by a combination of insulin, dexamethasone and isobutylmethylxanthine, IBMX, (I+D+M). Two transcription factors are important for the acquisition of the adipocyte phenotype, C/EBP beta (CCAT enhancer-binding protein beta) and PPAR gamma (peroxisome proliferator-activated receptor gamma). IBMX increases cAMP content, which can activate protein kinase A (PKA) and/or EPAC (exchange protein activated by cAMP). To investigate the importance of IBMX in the differentiation mixture, we first evaluated the effect of the addition of IBMX on the increase of C/EBP beta and PPAR gamma and found an enhancement of the amount of both proteins. IBMX addition (I+D+M) or its replacement with a cAMP analogue, dibutyryl-cAMP or 8-(4-chlorophenylthio)-2-O'-methyl-cAMP (8CPT-2-Me-cAMP), the latter activates EPAC and not PKA, remarkably increased PPAR gamma mRNA. However, neither I+D nor any of the inducers alone, increased PPAR gamma mRNA to a similar extent, suggesting the importance of the presence of both IBMX and I+D. It was also found that the addition of IBMX or 8CPT-2-Me-cAMP was able to increase the content of C/EBP beta with respect to I+D. In agreement with these findings, a microarray analysis showed that the presence of either 8CPT-2-Me-cAMP or IBMX in the differentiation mixture was able to upregulate PPAR gamma and PPAR gamma-activated genes as well as other genes involved in lipid metabolism. Our results prove the involvement of IBMX-cAMP-EPAC in the regulation of adipogenic genes during differentiation of 3T3-L1 fibroblasts and therfore contributes to elucidate the role of cyclic AMP in this process. © 2014 Japanese Society of Developmental Biologists. Fil:Gabrielli, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Martini, C.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iustman, L.J.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romero, D.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vila, M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00121592_v_n_p_Gabrielli http://hdl.handle.net/20.500.12110/paper_00121592_v_n_p_Gabrielli
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 3T3-L1 cells
Adipogenesis
CCAT enhancer-binding protein beta
Cyclic AMP
Peroxisome proliferator-activated receptor gamma
spellingShingle 3T3-L1 cells
Adipogenesis
CCAT enhancer-binding protein beta
Cyclic AMP
Peroxisome proliferator-activated receptor gamma
Gabrielli, Matías
Martini, Claudia Noemí
Raiger-Iustman, Laura Judith
Romero, Damián Gastón
Vila, María del Carmen
Exchange protein activated by cyclic AMP is involved in the regulation of adipogenic genes during 3T3-L1 fibroblasts differentiation
topic_facet 3T3-L1 cells
Adipogenesis
CCAT enhancer-binding protein beta
Cyclic AMP
Peroxisome proliferator-activated receptor gamma
description Adipogenesis is stimulated in 3T3-L1 fibroblasts by a combination of insulin, dexamethasone and isobutylmethylxanthine, IBMX, (I+D+M). Two transcription factors are important for the acquisition of the adipocyte phenotype, C/EBP beta (CCAT enhancer-binding protein beta) and PPAR gamma (peroxisome proliferator-activated receptor gamma). IBMX increases cAMP content, which can activate protein kinase A (PKA) and/or EPAC (exchange protein activated by cAMP). To investigate the importance of IBMX in the differentiation mixture, we first evaluated the effect of the addition of IBMX on the increase of C/EBP beta and PPAR gamma and found an enhancement of the amount of both proteins. IBMX addition (I+D+M) or its replacement with a cAMP analogue, dibutyryl-cAMP or 8-(4-chlorophenylthio)-2-O'-methyl-cAMP (8CPT-2-Me-cAMP), the latter activates EPAC and not PKA, remarkably increased PPAR gamma mRNA. However, neither I+D nor any of the inducers alone, increased PPAR gamma mRNA to a similar extent, suggesting the importance of the presence of both IBMX and I+D. It was also found that the addition of IBMX or 8CPT-2-Me-cAMP was able to increase the content of C/EBP beta with respect to I+D. In agreement with these findings, a microarray analysis showed that the presence of either 8CPT-2-Me-cAMP or IBMX in the differentiation mixture was able to upregulate PPAR gamma and PPAR gamma-activated genes as well as other genes involved in lipid metabolism. Our results prove the involvement of IBMX-cAMP-EPAC in the regulation of adipogenic genes during differentiation of 3T3-L1 fibroblasts and therfore contributes to elucidate the role of cyclic AMP in this process. © 2014 Japanese Society of Developmental Biologists.
author Gabrielli, Matías
Martini, Claudia Noemí
Raiger-Iustman, Laura Judith
Romero, Damián Gastón
Vila, María del Carmen
author_facet Gabrielli, Matías
Martini, Claudia Noemí
Raiger-Iustman, Laura Judith
Romero, Damián Gastón
Vila, María del Carmen
author_sort Gabrielli, Matías
title Exchange protein activated by cyclic AMP is involved in the regulation of adipogenic genes during 3T3-L1 fibroblasts differentiation
title_short Exchange protein activated by cyclic AMP is involved in the regulation of adipogenic genes during 3T3-L1 fibroblasts differentiation
title_full Exchange protein activated by cyclic AMP is involved in the regulation of adipogenic genes during 3T3-L1 fibroblasts differentiation
title_fullStr Exchange protein activated by cyclic AMP is involved in the regulation of adipogenic genes during 3T3-L1 fibroblasts differentiation
title_full_unstemmed Exchange protein activated by cyclic AMP is involved in the regulation of adipogenic genes during 3T3-L1 fibroblasts differentiation
title_sort exchange protein activated by cyclic amp is involved in the regulation of adipogenic genes during 3t3-l1 fibroblasts differentiation
publishDate 2014
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00121592_v_n_p_Gabrielli
http://hdl.handle.net/20.500.12110/paper_00121592_v_n_p_Gabrielli
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